High-Throughput Solid-Phase Building Block Synthesis for DNA-Encoded Libraries.

DNA / chemistry Gene Library Solid-Phase Synthesis Techniques / methods

Journal

Organic letters
ISSN: 1523-7052
Titre abrégé: Org Lett
Pays: United States
ID NLM: 100890393

Informations de publication

Date de publication:
06 12 2019
Historique:
pubmed: 21 11 2019
medline: 4 9 2020
entrez: 21 11 2019
Statut: ppublish

Résumé

Herein we provide a generalizable method for the cost-effective synthesis of thousands of building blocks (BBs) employing DNA-incompatible chemistries. The ability to produce large numbers of crude products via solid-phase synthesis has existed for decades; however, our work demonstrates a practical use of such crude reaction mixtures and employs DNA-conjugation to simultaneously encode, purify, and rapidly analyze the desired products. This workflow generated sp

Identifiants

DOI: 10.1021/acs.orglett.9b03553 PMID: 31747291
pubmed: 31747291
doi: 10.1021/acs.orglett.9b03553
doi:

Substances chimiques

DNA 9007-49-2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

9353-9357

Auteurs

Claudio Zambaldo (C)

Roche Innovation Center , Basel 4070 , Switzerland.

Stefanie N Geigle (SN)

Roche Innovation Center , Basel 4070 , Switzerland.

Alexander L Satz (AL)

Roche Innovation Center , Basel 4070 , Switzerland.
ORCID: 0000-0003-1284-1977

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Classifications MeSH

Recherchemedicale.com › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › High-Throughput Solid-Phase Building Block...
Recherchemedicale.com › Phénomènes génétiques › Structures génétiques › Banque de gènes › High-Throughput Solid-Phase Building Block...
Recherchemedicale.com › Technologie, industrie et agriculture › Technologie › Technologie pharmaceutique › Techniques de chimie synthétique › Techniques de synthèse en phase solide › High-Throughput Solid-Phase Building Block...