Platelet RNA modules point to coronary calcification in asymptomatic women with former preeclampsia.
Asymptomatic Diseases
Blood Coagulation
/ genetics
Blood Platelets
/ metabolism
Case-Control Studies
Coronary Artery Disease
/ blood
Early Diagnosis
Female
Gene Expression Profiling
Gene Regulatory Networks
Genetic Predisposition to Disease
Humans
Phenotype
Platelet Activation
/ genetics
Pre-Eclampsia
/ blood
Pregnancy
RNA
/ blood
Risk Factors
Transcriptome
Vascular Calcification
/ blood
Calcification
Network
Platelet
Preeclampsia
Transcriptome
Women
Journal
Atherosclerosis
ISSN: 1879-1484
Titre abrégé: Atherosclerosis
Pays: Ireland
ID NLM: 0242543
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
received:
11
03
2019
revised:
06
09
2019
accepted:
09
10
2019
pubmed:
11
11
2019
medline:
4
8
2020
entrez:
10
11
2019
Statut:
ppublish
Résumé
Women who develop preeclampsia during pregnancy are at a higher risk for developing cardiovascular disease. As platelets are affected by preeclampsia, we set out to identify whether platelets carry information in their transcriptome on cardiovascular risk in women with former preeclampsia. Platelets were isolated from asymptomatic women with previous preeclampsia, who underwent screening with coronary computed tomography angiography. Platelet RNA was isolated and used to construct gene networks using an unbiased approach. Platelet gene modules assembled from the network were related to risk factors and clinical traits of these women, including coronary artery calcium scores (CACS). We found multiple gene modules which correlated with CACS (correlation coefficients: 0.44 to 0.59, p = 0.05 to 0.007). The genes from two clinically relevant modules were expressed at a higher level in the group with calcifications (p = 3.9 × 10 We found platelet RNA modules that correlated with CACS in asymptomatic women with previous preeclampsia. Whether or not platelets directly contribute to this disease trajectory, or reflect the underlying plaque substrate remains to be determined, but enrichment for coronary artery disease susceptibility genes emphasizes the importance for the disease.
Sections du résumé
BACKGROUND AND AIMS
Women who develop preeclampsia during pregnancy are at a higher risk for developing cardiovascular disease. As platelets are affected by preeclampsia, we set out to identify whether platelets carry information in their transcriptome on cardiovascular risk in women with former preeclampsia.
METHODS
Platelets were isolated from asymptomatic women with previous preeclampsia, who underwent screening with coronary computed tomography angiography. Platelet RNA was isolated and used to construct gene networks using an unbiased approach. Platelet gene modules assembled from the network were related to risk factors and clinical traits of these women, including coronary artery calcium scores (CACS).
RESULTS
We found multiple gene modules which correlated with CACS (correlation coefficients: 0.44 to 0.59, p = 0.05 to 0.007). The genes from two clinically relevant modules were expressed at a higher level in the group with calcifications (p = 3.9 × 10
CONCLUSIONS
We found platelet RNA modules that correlated with CACS in asymptomatic women with previous preeclampsia. Whether or not platelets directly contribute to this disease trajectory, or reflect the underlying plaque substrate remains to be determined, but enrichment for coronary artery disease susceptibility genes emphasizes the importance for the disease.
Identifiants
pubmed: 31706077
pii: S0021-9150(19)31526-6
doi: 10.1016/j.atherosclerosis.2019.10.009
pii:
doi:
Substances chimiques
RNA
63231-63-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
114-121Informations de copyright
Copyright © 2019. Published by Elsevier B.V.