Resveratrol treatment in patients with polycystic ovary syndrome decreased pro-inflammatory and endoplasmic reticulum stress markers.
Activating Transcription Factor 4
/ genetics
Activating Transcription Factor 6
/ genetics
Adolescent
Adult
Anti-Inflammatory Agents
/ pharmacology
C-Reactive Protein
/ analysis
Cells, Cultured
Cumulus Cells
/ drug effects
Cytokines
/ blood
Double-Blind Method
Endoplasmic Reticulum Chaperone BiP
Endoplasmic Reticulum Stress
/ drug effects
Female
Heat-Shock Proteins
/ genetics
Humans
NF-kappa B
/ blood
Polycystic Ovary Syndrome
/ blood
Resveratrol
/ pharmacology
Transcription Factor CHOP
/ genetics
X-Box Binding Protein 1
/ genetics
Young Adult
endoplasmic reticulum stress
inflammation
polycystic ovary syndrome
resveratrol
Journal
American journal of reproductive immunology (New York, N.Y. : 1989)
ISSN: 1600-0897
Titre abrégé: Am J Reprod Immunol
Pays: Denmark
ID NLM: 8912860
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
28
08
2018
revised:
17
07
2019
accepted:
21
08
2019
pubmed:
5
9
2019
medline:
1
12
2020
entrez:
5
9
2019
Statut:
ppublish
Résumé
Polycystic ovary syndrome (PCOS) is associated with endoplasmic reticulum (ER) stress and pro-inflammatory condition. The aim of the present study was to evaluate the effect of resveratrol treatment on pro-inflammatory and ER stress markers in patients with PCOS. Cumulus cells were obtained from 40 patients with PCOS who were divided into two groups: placebo and resveratrol treatment (receiving 800 mg/d for 40 days) groups. Blood samples were obtained from all patients before and after the procedure to evaluate interleukin (IL)-6, IL-1β, IL-18, TNF-α, NF-κB, and C-reactive protein (CRP). Total RNA was extracted from cumulus cells, and cDNA was synthesized by reverse transcription. Expressions of five genes in ER stress response pathway (ATF4, ATF6, CHOP, GRP78, and XBP1s) were assessed with quantitative real-time PCR. Statistical analysis was performed with Student's t test. After treatment with resveratrol, it was found that serum levels of IL-6, IL-1β, TNF-α, IL-18, NF-κB, and CRP decreased in the treatment group. In addition, gene expression results showed that the expression levels of ATF4 (P < .05) and ATF6 (P < .001) significantly increased in the resveratrol treatment group, while the expression levels of CHOP, GRP78, and XBP1 (P < .001 for all) significantly decreased. Results demonstrated that resveratrol has anti-inflammatory effects through the suppression of NF-κB and NF-κB-regulated gene products. On the other hand, resveratrol can modulate ER stress in granulosa cells (GCs) by altering the expression of genes involved in unfolding protein response (UPR) process. Our findings suggest that ER stress is a potential therapeutic target for patients with PCOS.
Substances chimiques
ATF4 protein, human
0
ATF6 protein, human
0
Activating Transcription Factor 6
0
Anti-Inflammatory Agents
0
Cytokines
0
DDIT3 protein, human
0
Endoplasmic Reticulum Chaperone BiP
0
HSPA5 protein, human
0
Heat-Shock Proteins
0
NF-kappa B
0
X-Box Binding Protein 1
0
XBP1 protein, human
0
Activating Transcription Factor 4
145891-90-3
Transcription Factor CHOP
147336-12-7
C-Reactive Protein
9007-41-4
Resveratrol
Q369O8926L
Types de publication
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13186Subventions
Organisme : Tehran University of Medical Sciences and Health Services
ID : 94-03-30-29803
Pays : International
Informations de copyright
© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
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