Extracellular Vesicle-Induced Differentiation of Neural Stem Progenitor Cells.
EGF
astrocytes
basic FGF
exosomes
extracellular vesicles
neural stem progenitor cells
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
27 Jul 2019
27 Jul 2019
Historique:
received:
19
04
2019
revised:
24
07
2019
accepted:
25
07
2019
entrez:
31
7
2019
pubmed:
31
7
2019
medline:
10
1
2020
Statut:
epublish
Résumé
Neural stem progenitor cells (NSPCs) from E13.5 mouse embryos can be maintained in culture under proliferating conditions. Upon growth-factor removal, they may differentiate toward either neuronal or glial phenotypes or both. Exosomes are small extracellular vesicles that are part of the cell secretome; they may contain and deliver both proteins and genetic material and thus play a role in cell-cell communication, guide axonal growth, modulate synaptic activity and regulate peripheral nerve regeneration. In this work, we were interested in determining whether NSPCs and their progeny can produce and secrete extracellular vesicles (EVs) and if their content can affect cell differentiation. Our results indicate that cultured NSPCs produce and secrete EVs both under proliferating conditions and after differentiation. Treatment of proliferating NSPCs with EVs derived from differentiated NSPCs triggers cell differentiation in a dose-dependent manner, as demonstrated by glial- and neuronal-marker expression.
Identifiants
pubmed: 31357666
pii: ijms20153691
doi: 10.3390/ijms20153691
pmc: PMC6696602
pii:
doi:
Substances chimiques
Biomarkers
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Sapienza Università di Roma
ID : Progetti Ateneo 2016 and 2017
Références
Nat Rev Neurosci. 2002 Jul;3(7):517-30
pubmed: 12094208
Development. 1992 Sep;116(1):67-80
pubmed: 1483396
PLoS Biol. 2005 Sep;3(9):e283
pubmed: 16086633
Cereb Cortex. 2006 Jul;16 Suppl 1:i112-20
pubmed: 16766697
Neuron. 2007 Aug 2;55(3):417-33
pubmed: 17678855
Mol Cell Neurosci. 2008 Aug;38(4):595-606
pubmed: 18585058
Exp Mol Med. 2009 Feb 28;41(2):116-25
pubmed: 19287192
Curr Opin Cell Biol. 2009 Aug;21(4):575-81
pubmed: 19442504
Stem Cells Dev. 2013 Feb 1;22(3):345-58
pubmed: 23098139
PLoS One. 2012;7(12):e51798
pubmed: 23240065
PLoS One. 2013 Jul 11;8(7):e68693
pubmed: 23874726
Glia. 2013 Nov;61(11):1795-806
pubmed: 24038411
Stem Cells Dev. 2014 Jul 15;23(14):1625-35
pubmed: 24641099
Cell Death Differ. 2015 Jan;22(1):34-45
pubmed: 25236394
J Extracell Vesicles. 2014 Sep 30;3:null
pubmed: 25317276
J Neurosci. 2014 Nov 12;34(46):15482-9
pubmed: 25392515
J Extracell Vesicles. 2014 Nov 06;3:25011
pubmed: 25396408
Cell Biol Int. 2015 Apr;39(4):379-87
pubmed: 25484139
J Neurosci Res. 2015 Aug;93(8):1203-14
pubmed: 25691247
PLoS One. 2015 Aug 06;10(8):e0135111
pubmed: 26248331
Toxins (Basel). 2015 Nov 05;7(11):4610-21
pubmed: 26556375
Stem Cells Int. 2016;2016:1073140
pubmed: 26649044
Front Cell Neurosci. 2016 Jan 19;9:501
pubmed: 26834560
Biochim Biophys Acta. 2016 Jun;1858(6):1139-51
pubmed: 26874206
Curr Opin Neurobiol. 2016 Aug;39:101-7
pubmed: 27183381
PLoS One. 2017 Feb 2;12(2):e0171418
pubmed: 28152040
Stem Cells Int. 2017;2017:1719050
pubmed: 29081809
Nat Rev Mol Cell Biol. 2018 Apr;19(4):213-228
pubmed: 29339798
Dev Neurosci. 2018;40(3):223-233
pubmed: 29975945
Neurosci Lett. 2018 Oct 15;685:173-178
pubmed: 30153495
EBioMedicine. 2018 Dec;38:273-282
pubmed: 30472088
Cell. 1999 Jan 22;96(2):211-24
pubmed: 9988216