Influence of a Serratia marcescens outbreak on the gut microbiota establishment process in low-weight preterm neonates.
Cross Infection
/ microbiology
DNA, Bacterial
/ genetics
DNA, Ribosomal
/ genetics
Disease Outbreaks
Feces
/ microbiology
Female
Gastrointestinal Microbiome
/ genetics
Humans
Infant
Infant, Low Birth Weight
/ metabolism
Infant, Newborn
Infant, Premature
/ metabolism
Intensive Care Units, Neonatal
Male
Serratia Infections
/ microbiology
Serratia marcescens
/ genetics
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
10
11
2018
accepted:
25
04
2019
entrez:
22
5
2019
pubmed:
22
5
2019
medline:
24
1
2020
Statut:
epublish
Résumé
Adequate gut microbiota establishment is important for lifelong health. The aim was to sequentially analyze the gut microbiota establishment in low-birth-weight preterm neonates admitted to a single neonatal intensive care unit during their first 3 weeks of life, comparing two epidemiological scenarios. Seven control infants were recruited, and another 12 during a severe S. marcescens outbreak. Meconium and feces from days 7, 14, and 21 of life were collected. Gut microbiota composition was determined by 16S rDNA massive sequencing. Cultivable isolates were genotyped by pulsed-field gel electrophoresis, with four S. marcescens submitted for whole-genome sequencing. The expected bacterial ecosystem expansion after birth is delayed, possibly related to antibiotic exposure. The Proteobacteria phylum dominates, although with marked interindividual variability. The outbreak group considerably differed from the control group, with higher densities of Escherichia coli and Serratia to the detriment of Enterococcus and other Firmicutes. Curiously, obligate predators were only detected in meconium and at very low concentrations. Genotyping of cultivable bacteria demonstrated the high bacterial horizontal transmission rate that was confirmed with whole-genome sequencing for S. marcescens. Preterm infants admitted at NICU are initially colonized by homogeneous microbial communities, most of them from the nosocomial environment, which subsequently evolve according to the individual conditions. Our results demonstrate the hospital epidemiology pressure, particularly during outbreak situations, on the gut microbiota establishing process.
Identifiants
pubmed: 31112570
doi: 10.1371/journal.pone.0216581
pii: PONE-D-18-32393
pmc: PMC6529157
doi:
Substances chimiques
DNA, Bacterial
0
DNA, Ribosomal
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0216581Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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