Increased elasticity of melanoma cells after low-LET proton beam due to actin cytoskeleton rearrangements.
Actin Cytoskeleton
/ metabolism
Cell Line, Tumor
Cell Movement
/ radiation effects
Cell Proliferation
/ radiation effects
Cell Survival
/ radiation effects
Elasticity
/ radiation effects
Gene Expression Regulation, Neoplastic
/ radiation effects
Humans
Melanoma
/ metabolism
Proton Therapy
/ methods
Skin Neoplasms
/ metabolism
Up-Regulation
Uveal Neoplasms
/ metabolism
Vimentin
/ metabolism
Melanoma, Cutaneous Malignant
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
07 05 2019
07 05 2019
Historique:
received:
20
09
2018
accepted:
16
04
2019
entrez:
9
5
2019
pubmed:
9
5
2019
medline:
21
10
2020
Statut:
epublish
Résumé
Cellular response to non-lethal radiation stress include perturbations in DNA repair, angiogenesis, migration, and adhesion, among others. Low-LET proton beam radiation has been shown to induce somewhat different biological response than photon radiation. For example, we have shown that non-lethal doses of proton beam radiation inhibited migration of cells and that this effect persisted long-term. Here, we have examined cellular elasticity and actin cytoskeleton organization in BLM cutaneous melanoma and Mel270 uveal melanoma cells. Proton beam radiation increased cellular elasticity to a greater extent than X-rays and both types of radiation induced changes in actin cytoskeleton organization. Vimentin level increased in BLM cells after both types of radiation. Our data show that cell elasticity increased substantially after low-LET proton beam and persisted long after radiation. This may have significant consequences for the migratory properties of melanoma cells, as well as for the cell susceptibility to therapy.
Identifiants
pubmed: 31065009
doi: 10.1038/s41598-019-43453-7
pii: 10.1038/s41598-019-43453-7
pmc: PMC6504917
doi:
Substances chimiques
VIM protein, human
0
Vimentin
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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