Characterizing pre-transplant and post-transplant kidney rejection risk by B cell immune repertoire sequencing.
Adolescent
Adult
B-Lymphocytes
/ immunology
Child
Child, Preschool
Clone Cells
Female
Gene Expression
Graft Rejection
/ genetics
Graft Survival
/ genetics
Humans
Immunocompromised Host
Infant
Kidney
/ immunology
Kidney Transplantation
Longitudinal Studies
Male
Middle Aged
Polymorphism, Genetic
/ immunology
Receptors, Antigen, B-Cell
/ genetics
Renal Insufficiency, Chronic
/ genetics
Sequence Analysis, DNA
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
23 04 2019
23 04 2019
Historique:
received:
14
07
2018
accepted:
02
04
2019
entrez:
25
4
2019
pubmed:
25
4
2019
medline:
9
5
2019
Statut:
epublish
Résumé
Studying immune repertoire in the context of organ transplant provides important information on how adaptive immunity may contribute and modulate graft rejection. Here we characterize the peripheral blood immune repertoire of individuals before and after kidney transplant using B cell receptor sequencing in a longitudinal clinical study. Individuals who develop rejection after transplantation have a more diverse immune repertoire before transplant, suggesting a predisposition for post-transplant rejection risk. Additionally, over 2 years of follow-up, patients who develop rejection demonstrate a specific set of expanded clones that persist after the rejection. While there is an overall reduction of peripheral B cell diversity, likely due to increased general immunosuppression exposure in this cohort, the detection of specific IGHV gene usage across all rejecting patients supports that a common pool of immunogenic antigens may drive post-transplant rejection. Our findings may have clinical implications for the prediction and clinical management of kidney transplant rejection.
Identifiants
pubmed: 31015506
doi: 10.1038/s41467-019-09930-3
pii: 10.1038/s41467-019-09930-3
pmc: PMC6479061
doi:
Substances chimiques
Receptors, Antigen, B-Cell
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1906Subventions
Organisme : NIAMS NIH HHS
ID : P30 AR070155
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI113362
Pays : United States
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