Autophagy regulates lipid metabolism through selective turnover of NCoR1.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
05 04 2019
Historique:
received: 27 05 2018
accepted: 29 01 2019
entrez: 7 4 2019
pubmed: 7 4 2019
medline: 10 5 2019
Statut: epublish

Résumé

Selective autophagy ensures the removal of specific soluble proteins, protein aggregates, damaged mitochondria, and invasive bacteria from cells. Defective autophagy has been directly linked to metabolic disorders. However how selective autophagy regulates metabolism remains largely uncharacterized. Here we show that a deficiency in selective autophagy is associated with suppression of lipid oxidation. Hepatic loss of Atg7 or Atg5 significantly impairs the production of ketone bodies upon fasting, due to decreased expression of enzymes involved in β-oxidation following suppression of transactivation by PPARα. Mechanistically, nuclear receptor co-repressor 1 (NCoR1), which interacts with PPARα to suppress its transactivation, binds to the autophagosomal GABARAP family proteins and is degraded by autophagy. Consequently, loss of autophagy causes accumulation of NCoR1, suppressing PPARα activity and resulting in impaired lipid oxidation. These results suggest that autophagy contributes to PPARα activation upon fasting by promoting degradation of NCoR1 and thus regulates β-oxidation and ketone bodies production.

Identifiants

pubmed: 30952864
doi: 10.1038/s41467-019-08829-3
pii: 10.1038/s41467-019-08829-3
pmc: PMC6450892
doi:

Substances chimiques

Atg5 protein, mouse 0
Atg7 protein, mouse 0
Autophagy-Related Protein 5 0
Ketone Bodies 0
Nuclear Receptor Co-Repressor 1 0
PPAR alpha 0
Autophagy-Related Protein 7 EC 6.2.1.45

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1567

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Auteurs

Tetsuya Saito (T)

Department of Biochemistry, Niigata University Graduate School of Medical and Dental Sciences, Chuo-ku, Niigata, 951-8510, Japan.

Akiko Kuma (A)

Department of Biochemistry and Molecular Biology, Graduate School and Faculty of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan.
Department of Genetics, Graduate School of Medicine, Osaka University, Suita, Osaka, 565-0871, Japan.
Japan Science and Technology Agency, PRESTO, Saitama, 332-0012, Japan.

Yuki Sugiura (Y)

Japan Science and Technology Agency, PRESTO, Saitama, 332-0012, Japan.
Department of Biochemistry, Keio University School of Medicine, Tokyo, 160-8582, Japan.

Yoshinobu Ichimura (Y)

Department of Biochemistry, Niigata University Graduate School of Medical and Dental Sciences, Chuo-ku, Niigata, 951-8510, Japan.

Miki Obata (M)

Department of Biochemistry, Niigata University Graduate School of Medical and Dental Sciences, Chuo-ku, Niigata, 951-8510, Japan.

Hiroshi Kitamura (H)

Department of Gene Expression Regulation, Institute of Development, Aging and Cancer, Tohoku University, Sendai, 980-8575, Japan.

Shujiro Okuda (S)

Bioinformatics Laboratory, Niigata University Graduate School of Medical and Dental Sciences, Chuo-ku, Niigata, 951-8510, Japan.

Hyeon-Cheol Lee (HC)

Department of Biochemistry, Juntendo University Graduate School of Medicine, Bunkyo-ku, Tokyo, 113-8421, Japan.

Kazutaka Ikeda (K)

Laboratory for Metabolomics, RIKEN Center for Integrative Medical Sciences (IMS), Yokohama, Kanagawa, 230-0045, Japan.

Yumi Kanegae (Y)

Core Research Facilities of Basic Science (Molecular Genetics), Research Center for Medical Science, Jikei University School of Medicine, Tokyo, 105-8461, Japan.

Izumu Saito (I)

Laboratory of Molecular Genetics, Institute of Medical Science, The University of Tokyo, Tokyo, 108-8639, Japan.
Laboratory of Virology, Institute of Microbial Chemistry, Shinagawa-ku, Tokyo, 141-0021, Japan.

Johan Auwerx (J)

Laboratory of Integrative and Systems Physiology, École Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland.

Hozumi Motohashi (H)

Department of Gene Expression Regulation, Institute of Development, Aging and Cancer, Tohoku University, Sendai, 980-8575, Japan.

Makoto Suematsu (M)

Department of Biochemistry, Keio University School of Medicine, Tokyo, 160-8582, Japan.

Tomoyoshi Soga (T)

Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, 997-0052, Japan.

Takehiko Yokomizo (T)

Department of Biochemistry, Juntendo University Graduate School of Medicine, Bunkyo-ku, Tokyo, 113-8421, Japan.

Satoshi Waguri (S)

Department of Anatomy and Histology, Fukushima Medical University School of Medicine, Hikarigaoka, Fukushima, 960-1295, Japan.

Noboru Mizushima (N)

Department of Biochemistry and Molecular Biology, Graduate School and Faculty of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan.

Masaaki Komatsu (M)

Department of Biochemistry, Niigata University Graduate School of Medical and Dental Sciences, Chuo-ku, Niigata, 951-8510, Japan. mkomatsu@juntendo.ac.jp.
Department of Physiology, Juntendo University Graduate School of Medicine, Bunkyo-ku, Tokyo, 113-8421, Japan. mkomatsu@juntendo.ac.jp.

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