Host and Bacterial Markers that Differ in Children with Cystitis and Pyelonephritis.
UTI
calculator
diagnostic accuracy
prediction rule
risk
Journal
The Journal of pediatrics
ISSN: 1097-6833
Titre abrégé: J Pediatr
Pays: United States
ID NLM: 0375410
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
10
09
2018
revised:
02
01
2019
accepted:
04
01
2019
pubmed:
25
3
2019
medline:
17
4
2020
entrez:
26
3
2019
Statut:
ppublish
Résumé
To determine whether treatment for urinary tract infections in children could be individualized using biomarkers for acute pyelonephritis. We enrolled 61 children with febrile urinary tract infections, collected blood and urine samples, and performed a renal scan within 2 weeks of diagnosis to identify those with pyelonephritis. Renal scans were interpreted centrally by 2 experts. We measured inflammatory proteins in blood and urine using LUMINEX or an enzyme-linked immunosorbent assay. We evaluated serum RNA expression using RNA sequencing in a subset of children. Finally, for children with Escherichia coli isolated from urine cultures, we performed a polymerase chain reaction for 4 previously identified virulence genes. Urinary markers that best differentiated pyelonephritis from cystitis included chemokine (C-X-C motif) ligand (CXCL)1, CXCL9, CXCL12, C-C motif chemokine ligand 2, INF γ, and IL-15. Serum procalcitonin was the best serum marker for pyelonephritis. Genes in the interferon-γ pathway were upregulated in serum of children with pyelonephritis. The presence of E coli virulence genes did not correlate with pyelonephritis. Immune response to pyelonephritis and cystitis differs quantitatively and qualitatively; this may be useful in differentiating these 2 conditions.
Identifiants
pubmed: 30905425
pii: S0022-3476(19)30027-7
doi: 10.1016/j.jpeds.2019.01.012
pmc: PMC6535366
mid: NIHMS1518375
pii:
doi:
Substances chimiques
Biomarkers
0
Types de publication
Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
146-153.e1Subventions
Organisme : NIDDK NIH HHS
ID : R21 DK088672
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.
Références
J Urol. 1999 Jan;161(1):338-41
pubmed: 10037434
Curr Opin Microbiol. 1999 Feb;2(1):99-105
pubmed: 10047563
Pediatrics. 2000 Feb;105(2):E28
pubmed: 10654988
Behav Brain Res. 2001 Nov 1;125(1-2):279-84
pubmed: 11682119
Circulation. 2002 Sep 3;106(10):1219-23
pubmed: 12208796
Pediatr Infect Dis J. 1992 May;11(5):343-9
pubmed: 1352870
Br Med J. 1963 Aug 31;2(5356):524-7
pubmed: 14042765
Kidney Int. 2005 Jul;68(1):62-70
pubmed: 15954896
J Immunol. 2006 Mar 1;176(5):3080-6
pubmed: 16493067
Cytokine. 2006 Dec;36(5-6):276-82
pubmed: 17374489
Nephrology (Carlton). 2007 Oct;12(5):487-93
pubmed: 17803473
Pediatr Nephrol. 2008 Mar;23(3):429-33
pubmed: 18040727
Immunity. 2008 Jul 18;29(1):150-64
pubmed: 18631455
Pediatr Nephrol. 2009 Jan;24(1):105-11
pubmed: 18800229
J Comp Pathol. 2012 Jul;147(1):11-9
pubmed: 22014415
Immunity. 2013 Feb 21;38(2):349-59
pubmed: 23415912
Genome Biol. 2013 Apr 25;14(4):R36
pubmed: 23618408
Pediatrics. 2013 May;131(5):870-9
pubmed: 23629615
Korean J Pediatr. 2013 May;56(5):218-23
pubmed: 23741236
Eur J Pediatr. 2014 Feb;173(2):229-32
pubmed: 23918295
Cell Host Microbe. 2013 Dec 11;14(6):664-74
pubmed: 24331464
J Urol. 2014 Aug;192(2):600-6
pubmed: 24681329
Bioinformatics. 2014 Aug 1;30(15):2114-20
pubmed: 24695404
Pediatr Nephrol. 2014 Nov;29(11):2181-9
pubmed: 24924751
Eur J Pediatr. 2015 Jan;174(1):43-8
pubmed: 24965666
Pediatr Nephrol. 2015 Jan;30(1):123-30
pubmed: 25127917
Clin Exp Nephrol. 2015 Aug;19(4):688-93
pubmed: 25367868
Cochrane Database Syst Rev. 2015 Jan 20;1:CD009185
pubmed: 25603480
Pediatr Res. 2015 Jul;78(1):48-55
pubmed: 25790277
Clin Chem Lab Med. 2016 Jan;54(1):55-61
pubmed: 26053013
EBioMedicine. 2014 Oct 24;1(1):46-57
pubmed: 26125048
Nat Rev Immunol. 2015 Oct;15(10):655-63
pubmed: 26388331
J Immunol. 2015 Nov 15;195(10):4973-85
pubmed: 26453755
Pediatr Res. 2016 Jun;79(6):934-9
pubmed: 26885759
Electron Physician. 2016 Feb 25;8(2):1911-7
pubmed: 27053998
PLoS One. 2016 Apr 21;11(4):e0153252
pubmed: 27100186
Am J Physiol Renal Physiol. 2017 Jan 1;312(1):F43-F53
pubmed: 27760770
Microbiol Spectr. 2016 Dec;4(6):
pubmed: 28084200
Regen Biomater. 2017 Feb;4(1):55-68
pubmed: 28149530
Nephrol Dial Transplant. 2017 Dec 1;32(12):1977-1983
pubmed: 28340252
J Urol. 1988 Nov;140(5 Pt 2):1169-74
pubmed: 2846898
Cytokine. 2017 Nov;99:24-29
pubmed: 28683358
J Clin Microbiol. 1978 Nov;8(5):575-9
pubmed: 83326
J Nucl Med. 1996 Oct;37(10):1731-4
pubmed: 8862320