Muscle-Saturated Bioactive Lipids Are Increased with Aging and Influenced by High-Intensity Interval Training.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
12 Mar 2019
Historique:
received: 29 12 2018
revised: 06 03 2019
accepted: 07 03 2019
entrez: 16 3 2019
pubmed: 16 3 2019
medline: 27 6 2019
Statut: epublish

Résumé

Ceramide and diacylglycerol are linked to insulin resistance in rodents, but in humans the data are inconsistent. Insulin resistance is frequently observed with aging, but the role of ceramide and diacylglycerol is not clarified. Training improves metabolic health and, therefore, we aimed to elucidate the influence of age and high-intensity interval training (HIIT) on ceramide and diacylglycerol content in muscle. Fourteen young (33 ± 1) and 22 older (63 ± 1) overweight to obese subjects performed 6 weeks HIIT three times a week. Maximal oxygen uptake and body composition were measured and muscle biopsies and fasting blood samples were obtained. Muscle ceramide and diacylglycerol were measured by gas-liquid chromatography and proteins in insulin signaling, lipid and glucose metabolism were measured by Western blotting. Content of ceramide and diacylglycerol total, saturated, C16:0 and C18:0 fatty acids and C18:1 ceramide were higher in older compared to young. HIIT reduced saturated and C18:0 ceramides, while the content of the proteins involved in glucose (GLUT4, glycogen synthase, hexokinase II, AKT) and lipid metabolism (adipose triglyceride lipase, fatty acid binding protein) were increased after HIIT. We demonstrate a higher content of saturated ceramide and diacylglycerol fatty acids in the muscle of older subjects compared to young. Moreover, the content of saturated ceramides was reduced and muscle glucose metabolism improved at protein level after HIIT. This study highlights an increased content of saturated ceramides in aging which could be speculated to influence insulin sensitivity.

Identifiants

pubmed: 30871020
pii: ijms20051240
doi: 10.3390/ijms20051240
pmc: PMC6429484
pii:
doi:

Substances chimiques

1,2-diacylglycerol 0
Blood Glucose 0
Ceramides 0
Diglycerides 0
Fatty Acids 0
Insulin 0
Lipids 0
Glucose IY9XDZ35W2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Danish Diabetes Academy
ID : No grant number available

Références

Diabetes. 1999 Jun;48(6):1270-4
pubmed: 10342815
Am J Physiol Endocrinol Metab. 2001 Feb;280(2):E229-37
pubmed: 11158925
Metabolism. 2001 May;50(5):553-7
pubmed: 11319716
Diabetes Care. 2003 Jun;26(6):1706-13
pubmed: 12766098
J Biol Chem. 1957 May;226(1):497-509
pubmed: 13428781
Diabetes. 2004 May;53(5):1215-21
pubmed: 15111489
FASEB J. 2004 Jul;18(10):1144-6
pubmed: 15132977
J Biol Chem. 2004 Aug 27;279(35):36608-15
pubmed: 15220355
Am J Clin Nutr. 2004 Aug;80(2):475-82
pubmed: 15277173
Horm Metab Res. 2004 Sep;36(9):601-6
pubmed: 15486810
J Gerontol A Biol Sci Med Sci. 2005 Jul;60(7):872-7
pubmed: 16079210
Am J Physiol Endocrinol Metab. 2006 Jul;291(1):E99-E107
pubmed: 16464906
J Gerontol A Biol Sci Med Sci. 2006 Oct;61(10):1059-64
pubmed: 17077199
Am J Physiol Regul Integr Comp Physiol. 2007 May;292(5):R1970-6
pubmed: 17303684
Diabetologia. 2007 Nov;50(11):2366-73
pubmed: 17724577
Am J Physiol Endocrinol Metab. 2008 May;294(5):E882-8
pubmed: 18319352
Diabetologia. 2008 Jul;51(7):1253-60
pubmed: 18458871
Appl Physiol Nutr Metab. 2008 Dec;33(6):1112-23
pubmed: 19088769
J Clin Endocrinol Metab. 2009 Sep;94(9):3440-7
pubmed: 19531593
Diabetes. 2010 Jan;59(1):80-8
pubmed: 19833891
J Physiol. 2010 Aug 1;588(Pt 15):2961-72
pubmed: 20547683
Diabetologia. 2011 May;54(5):1147-56
pubmed: 21327867
Med Sci Sports Exerc. 2011 Oct;43(10):1849-56
pubmed: 21448086
Diabetes. 2011 Oct;60(10):2588-97
pubmed: 21873552
Diabetologia. 2012 Apr;55(4):1140-50
pubmed: 22252470
Diabetes. 2012 Dec;61(12):3148-55
pubmed: 22961081
J Appl Physiol (1985). 2012 Dec 1;113(11):1727-36
pubmed: 23042913
Diabetes Res Clin Pract. 2013 Feb;99(2):120-9
pubmed: 23183390
Obesity (Silver Spring). 2013 Nov;21(11):2362-71
pubmed: 23512750
Diabetes Obes Metab. 2013 Dec;15(12):1146-53
pubmed: 23802920
PLoS One. 2013 Jun 21;8(6):e66628
pubmed: 23805253
J Clin Endocrinol Metab. 2013 Dec;98(12):4852-62
pubmed: 24081737
J Clin Endocrinol Metab. 2013 Dec;98(12):4863-71
pubmed: 24178794
J Sports Med Phys Fitness. 2014 Apr;54(2):203-9
pubmed: 24509992
Scand J Med Sci Sports. 2015 Feb;25(1):e59-69
pubmed: 24845952
J Appl Physiol (1985). 2015 Aug 15;119(4):352-62
pubmed: 26112241
Diabetes. 2015 Nov;64(11):3737-50
pubmed: 26293505
Int J Sport Nutr Exerc Metab. 2016 Jun;26(3):197-204
pubmed: 26479856
PLoS One. 2015 Oct 21;10(10):e0138853
pubmed: 26489022
J Clin Endocrinol Metab. 2016 Feb;101(2):626-34
pubmed: 26709968
Diabetologia. 2016 Apr;59(4):785-98
pubmed: 26739815
Diabetes. 2016 Apr;65(4):840-50
pubmed: 26740597
Obesity (Silver Spring). 2016 Apr;24(4):908-16
pubmed: 26916476
J Physiol. 2016 Jun 15;594(12):3167-70
pubmed: 26996141
J Physiol. 2016 Jun 15;594(12):3171-4
pubmed: 26997581
J Diabetes Res. 2016;2016:2372741
pubmed: 27777958
Cell Metab. 2017 Mar 7;25(3):581-592
pubmed: 28273480
Acta Physiol (Oxf). 2018 Apr;222(4):e13009
pubmed: 29197155
J Gerontol A Biol Sci Med Sci. 2018 Sep 25;:null
pubmed: 30252030
J Lipid Res. 1980 Jan;21(1):139-44
pubmed: 7354251
J Appl Physiol (1985). 1994 Jun;76(6):2386-93
pubmed: 7928862
Lancet. 1979 Jan 20;1(8108):153
pubmed: 84172
Diabetes. 1993 Feb;42(2):273-81
pubmed: 8425663
Am J Clin Nutr. 1996 Jan;63(1):47-53
pubmed: 8604670
J Gerontol A Biol Sci Med Sci. 1996 Jul;51(4):B247-52
pubmed: 8680988
J Lipid Res. 1998 Oct;39(10):2091-8
pubmed: 9788256

Auteurs

Ditte Søgaard (D)

Xlab, Centre of Healthy Aging, Department of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen N, Denmark. dittek@sund.ku.dk.

Marcin Baranowski (M)

Department of Physiology, Medical University of Bialystock, 15-089 Bialystock, Poland. marcin.baranowski@umb.edu.pl.

Steen Larsen (S)

Xlab, Centre of Healthy Aging, Department of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen N, Denmark. stelar@sund.ku.dk.
Clinical Research Centre, Medical University of Bialystock, 15-089 Bialystock, Poland. stelar@sund.ku.dk.

Michael Taulo Lund (M)

Xlab, Centre of Healthy Aging, Department of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen N, Denmark. michaeltl@sund.ku.dk.

Cathrine Munk Scheuer (C)

Xlab, Centre of Healthy Aging, Department of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen N, Denmark. catrold@gmail.com.

Carina Vestergaard Abildskov (C)

Xlab, Centre of Healthy Aging, Department of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen N, Denmark. carina.vestergaard.abildskov@gmail.com.

Sofie Greve Dideriksen (S)

Xlab, Centre of Healthy Aging, Department of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen N, Denmark. sgdideriksen@gmail.com.

Flemming Dela (F)

Xlab, Centre of Healthy Aging, Department of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen N, Denmark. fdela@sund.ku.dk.
Department of Geriatrics, Bispebjerg University hospital, 2400 Copenhagen NV, Denmark. fdela@sund.ku.dk.

Jørn Wulff Helge (J)

Xlab, Centre of Healthy Aging, Department of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen N, Denmark. jhelge@sund.ku.dk.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female
Humans United States Aged Cross-Sectional Studies Medicare Part C
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH