Maturation of heart valve cell populations during postnatal remodeling.
Animals
Aortic Valve
/ growth & development
Cell Differentiation
Cell Lineage
Cluster Analysis
Collagen
/ chemistry
Endothelial Cells
/ cytology
Extracellular Matrix
/ chemistry
Female
Gene Expression Regulation, Developmental
Genetic Markers
Glycosaminoglycans
/ chemistry
Homeostasis
Humans
Immunohistochemistry
Male
Melanocytes
/ cytology
Mice
Mitral Valve
/ growth & development
Phenotype
Sequence Analysis, RNA
Single-Cell Analysis
/ methods
Swine
Tissue Engineering
/ methods
Transcriptome
Cardiovascular science
Development
Heart valve
Single cell RNA sequencing
Valve cell lineages
Journal
Development (Cambridge, England)
ISSN: 1477-9129
Titre abrégé: Development
Pays: England
ID NLM: 8701744
Informations de publication
Date de publication:
12 03 2019
12 03 2019
Historique:
received:
19
10
2018
accepted:
07
02
2019
pubmed:
24
2
2019
medline:
24
6
2020
entrez:
24
2
2019
Statut:
epublish
Résumé
Heart valve cells mediate extracellular matrix (ECM) remodeling during postnatal valve leaflet stratification, but phenotypic and transcriptional diversity of valve cells in development is largely unknown. Single cell analysis of mouse heart valve cells was used to evaluate cell heterogeneity during postnatal ECM remodeling and leaflet morphogenesis. The transcriptomic analysis of single cells from postnatal day (P)7 and P30 murine aortic (AoV) and mitral (MV) heart valves uncovered distinct subsets of melanocytes, immune and endothelial cells present at P7 and P30. By contrast, interstitial cell populations are different from P7 to P30. P7 valve leaflets exhibit two distinct collagen- and glycosaminoglycan-expressing interstitial cell clusters, and prevalent ECM gene expression. At P30, four interstitial cell clusters are apparent with leaflet specificity and differential expression of complement factors, ECM proteins and osteogenic genes. This initial transcriptomic analysis of postnatal heart valves at single cell resolution demonstrates that subpopulations of endothelial and immune cells are relatively constant throughout postnatal development, but interstitial cell subpopulations undergo changes in gene expression and cellular functions in primordial and mature valves.
Identifiants
pubmed: 30796046
pii: dev.173047
doi: 10.1242/dev.173047
pmc: PMC6602342
pii:
doi:
Substances chimiques
Genetic Markers
0
Glycosaminoglycans
0
Collagen
9007-34-5
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL094319
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL143881
Pays : United States
Informations de copyright
© 2019. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare no competing or financial interests.
Références
Circ Res. 2005 Apr 15;96(7):792-9
pubmed: 15761200
Anat Rec A Discov Mol Cell Evol Biol. 2005 Aug;285(2):748-57
pubmed: 15977222
Circ Res. 2006 Mar 17;98(5):690-6
pubmed: 16456103
Circulation. 2006 Mar 14;113(10):1344-52
pubmed: 16534030
Circ Res. 2006 Jun 9;98(11):1431-8
pubmed: 16645142
Dev Biol. 2007 May 1;305(1):120-32
pubmed: 17350610
Am J Pathol. 2007 Nov;171(5):1407-18
pubmed: 17823281
Circ Res. 2008 Apr 11;102(7):752-60
pubmed: 18296617
Circ Res. 2008 Oct 24;103(9):948-56
pubmed: 18802027
J R Soc Interface. 2009 Nov 6;6(40):1097-102
pubmed: 19586956
PLoS Comput Biol. 2009 Nov;5(11):e1000553
pubmed: 19893621
Genesis. 2011 Nov;49(11):870-7
pubmed: 21432986
J Mol Cell Cardiol. 2012 Mar;52(3):689-700
pubmed: 22248532
Dev Biol. 2014 Dec 1;396(1):8-18
pubmed: 25300579
Physiol Genomics. 2015 Mar;47(3):75-87
pubmed: 25547111
Cell. 2015 May 21;161(5):1202-1214
pubmed: 26000488
J Heart Valve Dis. 2015 Jul;24(4):451-6
pubmed: 26897815
Cells Tissues Organs. 2016;201(4):268-76
pubmed: 27144771
Arterioscler Thromb Vasc Biol. 2016 Jul;36(7):1398-405
pubmed: 27199449
Nature. 2016 Sep 29;537(7622):698-702
pubmed: 27580035
Cardiovasc Res. 2017 Jan;113(1):40-51
pubmed: 28069701
Am J Physiol Heart Circ Physiol. 2017 Dec 1;313(6):H1143-H1154
pubmed: 28842437
J Am Heart Assoc. 2017 Oct 20;6(10):null
pubmed: 29054843
Dev Cell. 2017 Nov 6;43(3):274-289.e5
pubmed: 29056552
Dev Biol. 2018 Feb 1;434(1):36-47
pubmed: 29183737
Cell Rep. 2018 Jan 16;22(3):600-610
pubmed: 29346760
Arterioscler Thromb Vasc Biol. 2018 Mar;38(3):636-644
pubmed: 29348122
Circulation. 2018 Jul 24;138(4):377-393
pubmed: 29588317
J Clin Invest. 2018 May 1;128(5):2127-2143
pubmed: 29664017
Front Cardiovasc Med. 2018 Apr 24;5:30
pubmed: 29740591
Ann Thorac Surg. 1980 Sep;30(3):230-9
pubmed: 7425702