In Vivo Formation of Stable Hyaline Cartilage by Naïve Human Bone Marrow Stromal Cells with Modified Fibrin Microbeads.
Animals
Cell Differentiation
Chondrogenesis
Fibrin
/ chemistry
Humans
Hyaline Cartilage
/ cytology
Hyaluronic Acid
/ chemistry
Immunocompromised Host
Mesenchymal Stem Cell Transplantation
Mesenchymal Stem Cells
/ cytology
Mice
Microspheres
Tissue Engineering
Tissue Scaffolds
/ chemistry
Transplantation, Heterologous
Bone marrow stromal cells
Cartilage formation
Fibrin microbeads
In vivo implantation
Journal
Stem cells translational medicine
ISSN: 2157-6580
Titre abrégé: Stem Cells Transl Med
Pays: England
ID NLM: 101578022
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
08
06
2018
accepted:
20
12
2018
pubmed:
16
2
2019
medline:
1
5
2020
entrez:
16
2
2019
Statut:
ppublish
Résumé
Osteoarthritic and other types of articular cartilage defects never heal on their own. Medicinal and surgical approaches are often ineffective, and the supply of autologous chondrocytes for tissue engineering is very limited. Bone marrow stromal cells (BMSCs, also known as bone marrow-derived mesenchymal stem cells) have been suggested as an adequate cell source for cartilage reconstruction. However, the majority of studies employing BMSCs for cartilage tissue engineering have used BMSCs predifferentiated into cartilage prior to implantation. This strategy has failed to achieve formation of stable, hyaline-like cartilage, resistant to hypertrophy in vivo. We hypothesized that in vitro predifferentiation of BMSCs is not necessary when cells are combined with an adequate scaffold that supports the formation of stable cartilage in vivo. In this study, naïve (undifferentiated) human BMSCs were attached to dehydrothermally crosslinked stable fibrin microbeads (FMBs) without and with other scaffolds and implanted subcutaneously into immunocompromised mice. Optimal formation of abundant, hypertrophy-resistant, ectopic hyaline-like cartilage was achieved when BMSCs were attached to FMBs covalently coated with hyaluronic acid. The cartilage that was formed was of human origin and was stable for at least 28 weeks in vivo. Stem Cells Translational Medicine 2019;8:586-592.
Identifiants
pubmed: 30767420
doi: 10.1002/sctm.18-0129
pmc: PMC6525579
doi:
Substances chimiques
Fibrin
9001-31-4
Hyaluronic Acid
9004-61-9
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
586-592Subventions
Organisme : Intramural NIH HHS
ID : ZIA DE000380
Pays : United States
Informations de copyright
© 2019 The Authors. Stem Cells Translational Medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press.
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