Combined quantification of intracellular (phospho-)proteins and transcriptomics from fixed single cells.
Cell Differentiation
Cells, Cultured
Focal Adhesion Kinase 1
/ genetics
Gene Expression Profiling
/ methods
Gene Expression Regulation
Humans
Keratinocytes
/ chemistry
Phosphorylation
Proteomics
/ methods
Quinazolines
/ pharmacology
Single-Cell Analysis
/ methods
Tissue Fixation
Tyrphostins
/ pharmacology
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
06 02 2019
06 02 2019
Historique:
received:
20
09
2018
accepted:
14
12
2018
entrez:
8
2
2019
pubmed:
8
2
2019
medline:
19
8
2020
Statut:
epublish
Résumé
Environmental stimuli often lead to heterogeneous cellular responses and transcriptional output. We developed single-cell RNA and Immunodetection (RAID) to allow combined analysis of the transcriptome and intracellular (phospho-)proteins from fixed single cells. RAID successfully recapitulated differentiation-state changes at the protein and mRNA level in human keratinocytes. Furthermore, we show that differentiated keratinocytes that retain high phosphorylated FAK levels, a feature associated with stem cells, also express a selection of stem cell associated transcripts. Our data demonstrates that RAID allows investigation of heterogeneous cellular responses to environmental signals at the mRNA and phospho-proteome level.
Identifiants
pubmed: 30728416
doi: 10.1038/s41598-018-37977-7
pii: 10.1038/s41598-018-37977-7
pmc: PMC6365588
doi:
Substances chimiques
Quinazolines
0
Tyrphostins
0
RTKI cpd
170449-18-0
Focal Adhesion Kinase 1
EC 2.7.10.2
PTK2 protein, human
EC 2.7.10.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1469Références
EMBO J. 2002 Aug 1;21(15):3919-26
pubmed: 12145193
Curr Opin Genet Dev. 2004 Feb;14(1):92-101
pubmed: 15108811
Tumour Biol. 2004 Jul-Aug;25(4):193-9
pubmed: 15557757
Nat Rev Mol Cell Biol. 2005 Apr;6(4):328-40
pubmed: 15803139
Br J Dermatol. 2005 Aug;153(2):274-81
pubmed: 16086736
Annu Rev Cell Dev Biol. 2006;22:339-73
pubmed: 16824012
Nat Protoc. 2007;2(8):1896-906
pubmed: 17703201
Nat Cell Biol. 2008 Aug;10(8):902-11
pubmed: 18604200
J Am Chem Soc. 2008 Oct 15;130(41):13518-9
pubmed: 18798613
Nat Biotechnol. 2008 Dec;26(12):1367-72
pubmed: 19029910
Genome Biol. 2010;11(2):R14
pubmed: 20132535
J Proteome Res. 2011 Jul 1;10(7):3040-9
pubmed: 21526778
Nat Cell Biol. 2012 Jun 24;14(7):753-63
pubmed: 22729083
Science. 2014 Feb 14;343(6172):776-9
pubmed: 24531970
Nat Biotechnol. 2014 Apr;32(4):381-386
pubmed: 24658644
Nat Med. 2014 Aug;20(8):847-56
pubmed: 25100530
Dev Cell. 2015 Mar 23;32(6):693-706
pubmed: 25805135
Cell. 2015 May 21;161(5):1187-1201
pubmed: 26000487
Cell. 2015 May 21;161(5):1202-1214
pubmed: 26000488
Nature. 2015 Sep 10;525(7568):251-5
pubmed: 26287467
Cell. 2015 Dec 17;163(7):1663-77
pubmed: 26627738
Nat Methods. 2016 Mar;13(3):269-75
pubmed: 26808670
Sci Rep. 2016 Mar 07;6:22675
pubmed: 26947912
Mol Cell. 2016 Mar 17;61(6):914-24
pubmed: 26990994
Genome Biol. 2016 Apr 28;17:77
pubmed: 27121950
Genome Biol. 2016 Sep 19;17(1):188
pubmed: 27640647
Cell Syst. 2016 Oct 26;3(4):385-394.e3
pubmed: 27693023
Sci Rep. 2017 Mar 14;7:44447
pubmed: 28290550
Nat Methods. 2017 Sep;14(9):865-868
pubmed: 28759029
Nat Biotechnol. 2017 Oct;35(10):936-939
pubmed: 28854175
Elife. 2017 Dec 05;6:
pubmed: 29206104
Sci Rep. 2018 Feb 1;8(1):2151
pubmed: 29391536
Nat Biotechnol. 2018 Jun;36(5):411-420
pubmed: 29608179
Nat Commun. 2018 Jun 19;9(1):2384
pubmed: 29921844