Evaluating of Induction of Apoptosis by Cornus mass L. Extract in the Gastric Carcinoma Cell Line (AGS)
Apoptosis
Gastric cancer
Cornus mass L. extract
L929 cells
AGS cell line
Journal
Asian Pacific journal of cancer prevention : APJCP
ISSN: 2476-762X
Titre abrégé: Asian Pac J Cancer Prev
Pays: Thailand
ID NLM: 101130625
Informations de publication
Date de publication:
25 Jan 2019
25 Jan 2019
Historique:
entrez:
26
1
2019
pubmed:
27
1
2019
medline:
18
5
2019
Statut:
epublish
Résumé
Aim and objectives: Natural products and derivatives of medicinal vegetation can play an important role to the
cure tumor. The Present study was focused to determine the effect of Cornus mass L. extract on the induction of
apoptosis in AGS gastric carcinoma cell line in compared to L929 cells. Methods: In this experimental study, AGS
and L929 cells were cultured and treated with different concentrations (0–10 mg/ml) of Cornus mass L. extract for 48
and 72 hours. Cell proliferation was assessed by MTT assay. The optical density of the colored solution was quantified
at 570 nm wavelengths by an ELISA Reader. Making use of the apoptosis detection kit of Annexin V-FITC, PI and
double staining with Annexin V-FITC were carried out for flow cytometry investigations. Data were analyzed by
ANOVA. Variations with a P-value less than 0.05 were considered significant. Results: shows a noticeable deviation
among various concentrations of extract when cells were treated for 48, 72 h declined cell viability in AGS cell line in
comparison L929 cell lines in a dose and time-dependent manner (P < 0.05). This extract also displayed approximately
several-fold increased anti-cancer potency in AGS compared to L929 cells. The IC50 value in AGS cells (evaluated
after 48,72h) of the extract against AGS cells was 5/44, 2/44 mg/ml (p≤0.05). The analysis results of flow cytometry
indicated that apoptosis was induced by the extract in AGS cells treated, compared with L929 cells. Conclusion: Each
of our results implicates the reality that Cornus mass L. extract acts as a novel, potent inhibitor of cancer proliferation
in in vitro. This may result in developing a promising therapeutic agent for the treatment of indole-sensitive cancers.
Identifiants
pubmed: 30678391
doi: 10.31557/APJCP.2019.20.1.123
pmc: PMC6485578
Substances chimiques
Plant Extracts
0
Types de publication
Evaluation Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
123-130Informations de copyright
Creative Commons Attribution License
Références
World J Gastroenterol. 2006 Jan 14;12(2):204-13
pubmed: 16482619
Nat Prod Res. 2009;23(18):1731-9
pubmed: 19921591
J Agric Food Chem. 2002 Apr 24;50(9):2519-23
pubmed: 11958615
Iran Biomed J. ;22(2):99-106
pubmed: 28800701
Food Chem. 2012 Dec 15;135(4):2675-80
pubmed: 22980857
Cytotechnology. 2017 Aug;69(4):551-563
pubmed: 28397098
Anticancer Res. 2018 Apr;38(4):2057-2067
pubmed: 29599323
Life Sci. 2017 Jul 15;181:31-44
pubmed: 28549559
Lipids Health Dis. 2013 May 10;12:71
pubmed: 23663688
Immunol Lett. 2017 Oct;190:7-14
pubmed: 28690187
Asian Pac J Cancer Prev. 2018 Aug 24;19(8):2217-2222
pubmed: 30139228
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2010 Feb;32(1):65-70
pubmed: 20236591
J Ethnopharmacol. 2012 Jul 13;142(2):456-61
pubmed: 22609155
Front Biosci (Landmark Ed). 2014 Jun 01;19(6):854-61
pubmed: 24896321
Life Sci. 2006 Jan 11;78(7):777-84
pubmed: 16139847
In Vitro Cell Dev Biol Anim. 2009 Jan-Feb;45(1-2):69-74
pubmed: 19015929
Asian Pac J Cancer Prev. 2014;15(8):3731-6
pubmed: 24870784
J Mol Neurosci. 2013 Jun;50(2):298-304
pubmed: 23335032
Clin Cancer Res. 2014 Oct 15;20(20):5290-301
pubmed: 24691018
J Biomed Res. 2017 Sep 26;31(5):468-470
pubmed: 28958999
Biometals. 2018 Apr;31(2):233-242
pubmed: 29429042
Biometals. 2018 Dec;31(6):981-990
pubmed: 30255365
Asian Pac J Cancer Prev. 2015;16(14):5727-31
pubmed: 26320443
Curr Opin Gastroenterol. 2010 Nov;26(6):640-6
pubmed: 20827183
Cancer Res. 1980 Feb;40(2):403-5
pubmed: 7356523
J Clin Oncol. 2006 May 10;24(14):2137-50
pubmed: 16682732
Asian Pac J Cancer Prev. 2018 Oct 26;19(10):2877-2884
pubmed: 30362316
Ther Adv Chronic Dis. 2012 Mar;3(2):87-106
pubmed: 23251771
CA Cancer J Clin. 2010 Sep-Oct;60(5):277-300
pubmed: 20610543
Inflammation. 2012 Jun;35(3):900-4
pubmed: 21968974
Radiat Res. 2018 May;189(5):456-465
pubmed: 29547353
Clin Cancer Res. 2001 Aug;7(8):2168-81
pubmed: 11489790
Oncol Lett. 2014 Apr;7(4):927-932
pubmed: 24944645
Ann N Y Acad Sci. 2009 Aug;1171:163-9
pubmed: 19723051
Pharm Dev Technol. 2017 Nov;22(7):881-888
pubmed: 26763623
JAMA. 2010 May 5;303(17):1729-37
pubmed: 20442389