The thrombin receptor (PAR1) is associated with microtubules, mitosis and process formation in glioma cells.

The cell surface protease-activated receptor 1 (PAR1) is overexpressed in glioblastoma multiforme (GBM). We studied the function and structure of intracellular microtubule (MT) and PAR1 in a tubulin-mediated process. We found that exposure to thrombin increased the percentage of proliferative, S, and M phases cells, affected morphology, and increased process elongation. PAR1 antagonist inversely affects these measures, increases tubulin end-binding protein 3 (EB3) mRNA expression in C6 cells, and reduces EB3 comet length, track length, and duration in neuroblastoma cells. In addition, immunofluorescence staining suggests that PAR1 is in close association with the MT α-tubulin and with coagulation cascade proteins during cell division stages. Our findings support PAR1 involvement in MT dynamics.

© 2024 The Authors.

The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Efrat Shavit-Stein reports financial support was provided by Israel Ministry of Innovation Science & Technology. Efrat Shavit-Stein, Joab Chapman has patent #PAR1 modulating molecules: “Compositions and methods for treating glioblastoma” issued to US-2021228681-A1.