Maternal and obstetric outcomes following the transfer of embryos warmed with fatty acid-supplemented solutions.
Cleavage-stage embryo transfer
Fatty acid
Live birth
Perinatal outcomes
Vitrification
Journal
BMC pregnancy and childbirth
ISSN: 1471-2393
Titre abrégé: BMC Pregnancy Childbirth
Pays: England
ID NLM: 100967799
Informations de publication
Date de publication:
04 May 2024
04 May 2024
Historique:
received:
01
01
2024
accepted:
26
04
2024
medline:
5
5
2024
pubmed:
5
5
2024
entrez:
4
5
2024
Statut:
epublish
Résumé
Vitrification procedures decrease intracytoplasmic lipid content and impair developmental competence. Adding fatty acids (FAs) to the warming solution has been shown to recover the lipid content of the cytoplasm and improve developmental competence and pregnancy outcomes. However, the influence of the FA supplementation on live birth rates after embryo transfers and perinatal outcomes remains unknown. In the present study, we examined the influence of FA-supplemented warming solutions on live birth rates, pregnancy complications, and neonatal outcomes after single vitrified-warmed cleavage-stage embryo transfers (SVCTs). The clinical records of 701 treatment cycles in 701 women who underwent SVCTs were retrospectively analyzed. Vitrified embryos were warmed using solutions (from April 2022 to June 2022, control group) or FA-supplemented solutions (from July 2022 to September 2022, FA group). The live birth rate, pregnancy complications, and perinatal outcomes were compared between the control and FA groups. The live birth rate per transfer was significantly higher in the FA group than in the control group. Multivariate logistic regression analysis further demonstrated a higher probability of live births in the FA group than in the control group. Miscarriage rates, the incidence and types of pregnancy complications, the cesarean section rate, gestational age, incidence of preterm delivery, birth length and weight, incidence of low birth weight, infant sex, and incidence of birth defects were all comparable between the control and FA groups. Multivariate logistic regression analysis further demonstrated no adverse effects of FA-supplemented warming solutions. FA-supplemented warming solutions improved live birth rates after SVCTs without exerting any adverse effects on maternal and obstetric outcomes. Therefore, FA-supplemented solutions can be considered safe and effective for improving clinical outcomes and reducing patient burden.
Sections du résumé
BACKGROUND
BACKGROUND
Vitrification procedures decrease intracytoplasmic lipid content and impair developmental competence. Adding fatty acids (FAs) to the warming solution has been shown to recover the lipid content of the cytoplasm and improve developmental competence and pregnancy outcomes. However, the influence of the FA supplementation on live birth rates after embryo transfers and perinatal outcomes remains unknown. In the present study, we examined the influence of FA-supplemented warming solutions on live birth rates, pregnancy complications, and neonatal outcomes after single vitrified-warmed cleavage-stage embryo transfers (SVCTs).
METHODS
METHODS
The clinical records of 701 treatment cycles in 701 women who underwent SVCTs were retrospectively analyzed. Vitrified embryos were warmed using solutions (from April 2022 to June 2022, control group) or FA-supplemented solutions (from July 2022 to September 2022, FA group). The live birth rate, pregnancy complications, and perinatal outcomes were compared between the control and FA groups.
RESULTS
RESULTS
The live birth rate per transfer was significantly higher in the FA group than in the control group. Multivariate logistic regression analysis further demonstrated a higher probability of live births in the FA group than in the control group. Miscarriage rates, the incidence and types of pregnancy complications, the cesarean section rate, gestational age, incidence of preterm delivery, birth length and weight, incidence of low birth weight, infant sex, and incidence of birth defects were all comparable between the control and FA groups. Multivariate logistic regression analysis further demonstrated no adverse effects of FA-supplemented warming solutions.
CONCLUSIONS
CONCLUSIONS
FA-supplemented warming solutions improved live birth rates after SVCTs without exerting any adverse effects on maternal and obstetric outcomes. Therefore, FA-supplemented solutions can be considered safe and effective for improving clinical outcomes and reducing patient burden.
Identifiants
pubmed: 38704546
doi: 10.1186/s12884-024-06546-4
pii: 10.1186/s12884-024-06546-4
doi:
Substances chimiques
Fatty Acids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
343Informations de copyright
© 2024. The Author(s).
Références
Katagiri Y, Jwa SC, Kuwahara A, Iwasa T, Ono M, Kato K, et al. Assisted reproductive technology in Japan: a summary report for 2020 by the ethics Committee of the Japan Society of obstetrics and gynecology. Reprod Med Biol. 2023;22:e12494.
doi: 10.1002/rmb2.12494
pubmed: 36618448
pmcid: 9811980
De Geyter C, Wyns C, Calhaz-Jorge C, de Mouzon J, Ferraretti AP, Kupka M, et al. 20 years of the European IVF-monitoring Consortium registry: what have we learned? A comparison with registries from two other regions. Hum Reprod. 2020;35:2832–49.
doi: 10.1093/humrep/deaa250
pubmed: 33188410
pmcid: 7744162
European IVFMCftESoHR, Embryology, Smeenk J, Wyns C, De Geyter C, Kupka M et al. ART in Europe, 2019: results generated from European registries by ESHREdagger. Hum Reprod. 2023.
Bang S, Shin H, Song H, Suh CS, Lim HJ. Autophagic activation in vitrified-warmed mouse oocytes. Reproduction. 2014;148:11–9.
doi: 10.1530/REP-14-0036
pubmed: 24760879
Oktay K, Cil AP, Bang H. Efficiency of oocyte cryopreservation: a meta-analysis. Fertil Steril. 2006;86:70–80.
doi: 10.1016/j.fertnstert.2006.03.017
pubmed: 16818031
Zander-Fox D, Cashman KS, Lane M. The presence of 1 mM glycine in vitrification solutions protects oocyte mitochondrial homeostasis and improves blastocyst development. J Assist Reprod Genet. 2013;30:107–16.
doi: 10.1007/s10815-012-9898-4
pubmed: 23248076
Ducibella T, Fissore R. The roles of Ca2+, downstream protein kinases, and oscillatory signaling in regulating fertilization and the activation of development. Dev Biol. 2008;315:257–79.
doi: 10.1016/j.ydbio.2007.12.012
pubmed: 18255053
pmcid: 4276041
Larman MG, Sheehan CB, Gardner DK. Calcium-free vitrification reduces cryoprotectant-induced zona pellucida hardening and increases fertilization rates in mouse oocytes. Reproduction. 2006;131:53–61.
doi: 10.1530/rep.1.00878
pubmed: 16388009
Tamura AN, Huang TT, Marikawa Y. Impact of vitrification on the meiotic spindle and components of the microtubule-organizing center in mouse mature oocytes. Biol Reprod. 2013;89:112.
doi: 10.1095/biolreprod.113.108167
pubmed: 24025740
pmcid: 4076379
Ohata K, Ezoe K, Miki T, Kouraba S, Fujiwara N, Yabuuchi A, et al. Effects of fatty acid supplementation during vitrification and warming on the developmental competence of mouse, bovine and human oocytes and embryos. Reprod Biomed Online. 2021;43:14–25.
doi: 10.1016/j.rbmo.2021.03.022
pubmed: 34049810
Maheshwari A, Pandey S, Amalraj Raja E, Shetty A, Hamilton M, Bhattacharya S. Is frozen embryo transfer better for mothers and babies? Can cumulative meta-analysis provide a definitive answer? Hum Reprod Update. 2018;24:35–58.
doi: 10.1093/humupd/dmx031
pubmed: 29155965
Ishihara O, Araki R, Kuwahara A, Itakura A, Saito H, Adamson GD. Impact of frozen-thawed single-blastocyst transfer on maternal and neonatal outcome: an analysis of 277,042 single-embryo transfer cycles from 2008 to 2010 in Japan. Fertil Steril. 2014;101:128–33.
doi: 10.1016/j.fertnstert.2013.09.025
pubmed: 24268706
Qin JB, Sheng XQ, Wu D, Gao SY, You YP, Yang TB, et al. Worldwide prevalence of adverse pregnancy outcomes among singleton pregnancies after in vitro fertilization/intracytoplasmic sperm injection: a systematic review and meta-analysis. Arch Gynecol Obstet. 2017;295:285–301.
doi: 10.1007/s00404-016-4250-3
pubmed: 27896474
Romundstad LB, Romundstad PR, Sunde A, von During V, Skjaerven R, Vatten LJ. Increased risk of placenta previa in pregnancies following IVF/ICSI; a comparison of ART and non-ART pregnancies in the same mother. Hum Reprod. 2006;21:2353–8.
doi: 10.1093/humrep/del153
pubmed: 16728419
Vermey BG, Buchanan A, Chambers GM, Kolibianakis EM, Bosdou J, Chapman MG, et al. Are singleton pregnancies after assisted reproduction technology (ART) associated with a higher risk of placental anomalies compared with non-ART singleton pregnancies? A systematic review and meta-analysis. BJOG. 2019;126:209–18.
doi: 10.1111/1471-0528.15227
pubmed: 29740927
Coughlan C, Ledger W, Wang Q, Liu F, Demirol A, Gurgan T, et al. Recurrent implantation failure: definition and management. Reprod Biomed Online. 2014;28:14–38.
doi: 10.1016/j.rbmo.2013.08.011
pubmed: 24269084
Amagai A, Ezoe K, Miki T, Shimazaki K, Okimura T, Kato K. Fatty acid supplementation into warming solutions improves pregnancy outcomes after single vitrified-warmed cleavage stage embryo transfers. Reprod Med Biol. 2023;22:e12517.
doi: 10.1002/rmb2.12517
pubmed: 37168396
pmcid: 10165886
Onogi S, Ezoe K, Kawasaki N, Hayashi H, Kuroda T, Takeshima K, et al. Maternal and obstetric outcomes are influenced by developmental stage and cryopreservation of transferred embryos after clomiphene citrate-based minimal stimulation IVF. Hum Reprod Open. 2022;2022:hoac018.
doi: 10.1093/hropen/hoac018
pubmed: 35591922
pmcid: 9113344
Ezoe K, Fukuda J, Takeshima K, Shinohara K, Kato K. Letrozole-induced endometrial preparation improved the pregnancy outcomes after frozen blastocyst transfer compared to the natural cycle: a retrospective cohort study. BMC Pregnancy Childbirth. 2022;22:824.
doi: 10.1186/s12884-022-05174-0
pubmed: 36344952
pmcid: 9639274
Takeshima K, Ezoe K, Kawasaki N, Hayashi H, Kuroda T, Kato K. Perinatal outcomes and congenital anomalies associated with letrozole and natural cycles in single fresh cleaved embryo transfers: a single-center, 10-year cohort study. F S Rep. 2022;3:138–44.
pubmed: 35789728
pmcid: 9250119
Jansen C, Kleinrouweler CE, Kastelein AW, Ruiter L, van Leeuwen E, Mol BW, et al. Follow-up ultrasound in second-trimester low-positioned anterior and posterior placentae: prospective cohort study. Ultrasound Obstet Gynecol. 2020;56:725–31.
doi: 10.1002/uog.21903
pubmed: 31671480
pmcid: 7702149
Itabashi K, Fujimura M, Kusuda S, Tamura M, Hayashi T, Takahashi T et al. New standard of average size and weight of newborn in Japan. Jap J Pediat 114:1271–93.
World Health Organization. Internatilnal Stastical Classification of Diseases and Related Health Problems 10th Revison. 2016. https://apps.who.int/iris/handle/10665/246208 . Accessed September 20 2021.
EUROCAT. EUROCAT Guide 1.5: EUROCAT Subgroups of Congenital Anomalies In: EUROCAT Central Registry. EUROCAT Central Registry. 2022. https://eu-rd-platform.jrc.ec.europa.eu/system/files/public/eurocat/Guide_1.5_Chapter_3.3_June_2022.pdf .
Van Blerkom J. Mitochondria in human oogenesis and preimplantation embryogenesis: engines of metabolism, ionic regulation and developmental competence. Reproduction. 2004;128:269–80.
doi: 10.1530/rep.1.00240
pubmed: 15333778
Dunning KR, Cashman K, Russell DL, Thompson JG, Norman RJ, Robker RL. Beta-oxidation is essential for mouse oocyte developmental competence and early embryo development. Biol Reprod. 2010;83:909–18.
doi: 10.1095/biolreprod.110.084145
pubmed: 20686180
Houten SM, Wanders RJ. A general introduction to the biochemistry of mitochondrial fatty acid beta-oxidation. J Inherit Metab Dis. 2010;33:469–77.
doi: 10.1007/s10545-010-9061-2
pubmed: 20195903
pmcid: 2950079
Wanders RJ. Peroxisomes, lipid metabolism, and peroxisomal disorders. Mol Genet Metab. 2004;83:16–27.
doi: 10.1016/j.ymgme.2004.08.016
pubmed: 15464416
Hashimoto S, Morimoto Y. Mitochondrial function of human embryo: decline in their quality with maternal aging. Reprod Med Biol. 2022;21:e12491.
doi: 10.1002/rmb2.12491
pubmed: 36570768
pmcid: 9769491
Takeshima K, Ezoe K, Onogi S, Kawasaki N, Hayashi H, Kuroda T, et al. Endometrial preparation and maternal and obstetrical outcomes after frozen blastocyst transfer. AJOG Glob Rep. 2022;2:100081.
doi: 10.1016/j.xagr.2022.100081
pubmed: 36387298
pmcid: 9664017
Ibayashi M, Tsukamoto S. Lipid remodelling in mammalian development. Nat Cell Biol. 2024.
Aizawa R, Ibayashi M, Tatsumi T, Yamamoto A, Kokubo T, Miyasaka N et al. Synthesis and maintenance of lipid droplets are essential for mouse preimplantation embryonic development. Development. 2019;146.
Ibayashi M, Aizawa R, Mitsui J, Tsukamoto S. Homeostatic regulation of lipid droplet content in mammalian oocytes and embryos. Reproduction. 2021;162:R99–109.
doi: 10.1530/REP-21-0238
pubmed: 34715675
Talari NK, Mattam U, Meher NK, Paripati AK, Mahadev K, Krishnamoorthy T, et al. Lipid-droplet associated mitochondria promote fatty-acid oxidation through a distinct bioenergetic pattern in male Wistar rats. Nat Commun. 2023;14:766.
doi: 10.1038/s41467-023-36432-0
pubmed: 36765117
pmcid: 9918515
Zhang L, Zhao J, Lam SM, Chen L, Gao Y, Wang W et al. Low-input lipidomics reveals lipid metabolism remodelling during early mammalian embryo development. Nat Cell Biol. 2024.