Molecular detection of exosomal miRNAs of blood serum for prognosis of colorectal cancer.

Humans MicroRNAs / genetics Serum / metabolism Colorectal Neoplasms / pathology Prognosis Biomarkers / metabolism Exosomes / metabolism
Biomarkers Colorectal cancer Deep sequencing Exosomes miRNAs

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
17 04 2024
Historique:
received: 15 10 2023
accepted: 01 04 2024
medline: 19 4 2024
pubmed: 18 4 2024
entrez: 17 4 2024
Statut: epublish

Résumé

Colorectal cancer (CRC) is the third most common cancer affecting people. The discovery of new, non-invasive, specific, and sensitive molecular biomarkers for CRC may assist in the diagnosis and support therapeutic decision making. Exosomal miRNAs have been demonstrated in carcinogenesis and CRC development, which makes these miRNAs strong biomarkers for CRC. Deep sequencing allows a robust high-throughput informatics investigation of the types and abundance of exosomal miRNAs. Thus, exosomal miRNAs can be efficiently examined as diagnostic biomarkers for disease screening. In the present study, a number of 660 mature miRNAs were detected in patients diagnosed with CRC at different stages. Of which, 29 miRNAs were differentially expressed in CRC patients compared with healthy controls. Twenty-nine miRNAs with high abundance levels were further selected for subsequent analysis. These miRNAs were either highly up-regulated (e.g., let-7a-5p, let-7c-5p, let-7f-5p, let-7d-3p, miR-423-5p, miR-3184-5p, and miR-584) or down-regulated (e.g., miR-30a-5p, miR-99-5p, miR-150-5p, miR-26-5p and miR-204-5p). These miRNAs influence critical genes in CRC, leading to either tumor growth or suppression. Most of the reported diagnostic exosomal miRNAs were shown to be circulating in blood serum. The latter is a novel miRNA that was found in exosomal profile of blood serum. Some of the predicted target genes of highly expressed miRNAs participate in several cancer pathways, including CRC pathway. These target genes include tumor suppressor genes, oncogenes and DNA repair genes. Main focus was given to multiple critical signaling cross-talking pathways including transforming growth factor β (TGFβ) signaling pathways that are directly linked to CRC. In conclusion, we recommend further analysis in order to experimentally confirm exact relationships between selected differentially expressed miRNAs and their predicted target genes and downstream functional consequences.

Identifiants

DOI: 10.1038/s41598-024-58536-3 PMID: 38632250 PMC: PMC11024162
pubmed: 38632250
doi: 10.1038/s41598-024-58536-3
pii: 10.1038/s41598-024-58536-3
pmc: PMC11024162
doi:

Substances chimiques

MicroRNAs 0
Biomarkers 0
MIRN204 microRNA, human 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

8902

Informations de copyright

© 2024. The Author(s).

Références

Cancer Metastasis Rev. 2017 Sep;36(3):525-545
pubmed: 28836104
J Biomed Sci. 2014 Jan 08;21:1
pubmed: 24397824
Cell Death Dis. 2017 Jun 22;8(6):e2892
pubmed: 28640257
World J Gastroenterol. 2014 Jun 14;20(22):6786-808
pubmed: 24944469
Cell. 2000 Oct 13;103(2):295-309
pubmed: 11057902
PLoS Comput Biol. 2010 Apr 01;6(4):e1000730
pubmed: 20369013
Arch Biochem Biophys. 2018 Sep 15;654:47-54
pubmed: 30009782
PLoS One. 2010 Jun 30;5(6):e11387
pubmed: 20613982
Cell Physiol Biochem. 2013;31(2-3):209-18
pubmed: 23486085
Int J Mol Sci. 2019 Sep 21;20(19):
pubmed: 31546654
World J Gastroenterol. 2015 Jan 7;21(1):84-93
pubmed: 25574081
Nucleic Acids Res. 2012 Oct;40(18):9125-38
pubmed: 22772984
Biomed Pharmacother. 2020 Jan;121:109495
pubmed: 31731194
Genes Dev. 2003 Apr 15;17(8):991-1008
pubmed: 12672692
Pathol Res Pract. 2018 May;214(5):700-705
pubmed: 29567332
Annu Rev Cell Dev Biol. 2005;21:659-93
pubmed: 16212511
J Cell Biochem. 2019 Dec;120(12):19245-19253
pubmed: 31512778
J Cell Mol Med. 2014 Oct;18(10):2125-34
pubmed: 25230975
Nat Med. 2015 Nov;21(11):1350-6
pubmed: 26457759
Talanta. 2013 Jun 15;110:190-5
pubmed: 23618193
Cell Physiol Biochem. 2013;32(5):1331-41
pubmed: 24280681
Cancer Res. 2013 Jan 15;73(2):725-35
pubmed: 23139211
Sci Rep. 2018 Jan 8;8(1):1
pubmed: 29311619
Oncogene. 2010 Jul 22;29(29):4237-44
pubmed: 20498632
Int J Mol Sci. 2018 Jun 28;19(7):
pubmed: 29958433
Nat Rev Mol Cell Biol. 2019 Jan;20(1):5-20
pubmed: 30228348
Pancreas. 2015 Jul;44(5):764-8
pubmed: 25906450
J Cell Biochem. 2019 May;120(5):7962-7969
pubmed: 30548660
Mol Cancer. 2014 Jan 03;13:1
pubmed: 24387052
Exp Mol Med. 2020 Nov;52(11):1809-1822
pubmed: 33144675
Oncotarget. 2015 Sep 15;6(27):24448-62
pubmed: 26259252
Gastroenterol Hepatol Bed Bench. 2017 Spring;10(2):112-117
pubmed: 28702135
EBioMedicine. 2018 Sep;35:189-197
pubmed: 30166271
Am J Clin Pathol. 2005 Apr;123(4):562-70
pubmed: 15743744
Cancer Med. 2019 May;8(5):2484-2495
pubmed: 30938104
Cancer Biomark. 2019;24(3):299-305
pubmed: 30829615
Int J Mol Med. 2018 Dec;42(6):3009-3016
pubmed: 30221678
Biochem Biophys Res Commun. 2016 Apr 8;472(3):465-70
pubmed: 26827826
Nat Struct Mol Biol. 2008 Apr;15(4):354-63
pubmed: 18376413
Mol Med Rep. 2018 Apr;17(4):5143-5149
pubmed: 29393430
Carcinogenesis. 2014 Apr;35(4):776-83
pubmed: 24242331
Clin Cancer Res. 2017 Jan 1;23(1):159-170
pubmed: 27401248
Nucleic Acids Res. 2012 Jan;40(1):37-52
pubmed: 21911355
Aging (Albany NY). 2019 Apr 12;11(7):2138-2150
pubmed: 30981205
Cell. 2005 Mar 11;120(5):635-47
pubmed: 15766527
Front Genet. 2017 Mar 28;8:31
pubmed: 28400788
Biochem Biophys Rep. 2021 Jul 20;27:101079
pubmed: 34355069
Clin Cancer Res. 2017 Sep 1;23(17):5311-5319
pubmed: 28606918
Cell Physiol Biochem. 2015;37(3):1044-54
pubmed: 26402653
Cancers (Basel). 2013 Jun 05;5(2):676-713
pubmed: 24216997
Biochem Biophys Res Commun. 2019 Jan 22;508(4):1259-1263
pubmed: 30563768
J Cancer Res Clin Oncol. 2017 Oct;143(10):1915-1927
pubmed: 28528497
Development. 2016 Jul 15;143(14):2482-93
pubmed: 27436038
Oncol Lett. 2016 Feb;11(2):1406-1412
pubmed: 26893751
Oncotarget. 2017 Apr 4;8(14):22305-22306
pubmed: 28423591
Acta Oncol. 2014 Jul;53(7):852-64
pubmed: 24666267
Oncotarget. 2016 Nov 15;7(46):76250-76260
pubmed: 27788488
Neuro Oncol. 2012 Aug;14(8):1007-17
pubmed: 22718995
Genomics. 2019 Jul;111(4):762-771
pubmed: 29860032
PLoS One. 2019 Aug 30;14(8):e0222013
pubmed: 31469874
J Cell Physiol. 2018 Aug;233(8):5574-5588
pubmed: 29521426
Cancer Sci. 2018 Feb;109(2):354-362
pubmed: 29160937
Cancer Res. 2013 Jan 15;73(2):990-9
pubmed: 23204229
Int J Biol Sci. 2018 Jan 12;14(2):111-123
pubmed: 29483830
Cell. 2018 Mar 22;173(1):20-51
pubmed: 29570994
PLoS One. 2013 Jun 18;8(6):e66165
pubmed: 23824282
Cancer Cell Int. 2020 Jan 03;20:1
pubmed: 31908598
Wiley Interdiscip Rev RNA. 2021 May;12(3):e1635
pubmed: 33230974
J Pathol. 2012 Feb;226(3):544-55
pubmed: 21984339
Cancer Cell. 2012 Apr 17;21(4):532-46
pubmed: 22516261
Int J Oncol. 2018 Mar;52(3):913-924
pubmed: 29286099
PLoS One. 2015 Nov 12;10(11):e0142904
pubmed: 26562412
Clin Cancer Res. 2014 Dec 1;20(23):6187-99
pubmed: 25294901
Mol Cancer. 2013 Jan 03;12:1
pubmed: 23286373
Gene. 2019 Mar 10;688:93-97
pubmed: 30415005
PLoS One. 2014 Apr 04;9(4):e92921
pubmed: 24705249
Biochem Biophys Res Commun. 2015 Nov 27;467(4):847-52
pubmed: 26494299
Cancer Res. 2009 Nov 1;69(21):8317-25
pubmed: 19861538
PLoS One. 2013 Dec 02;8(12):e80707
pubmed: 24312495
Biochimie. 2019 Feb;157:64-71
pubmed: 30414835
Gut. 2012 Oct;61(10):1447-53
pubmed: 22052060
Cancer Sci. 2013 Jan;104(1):48-54
pubmed: 23013135
Cell. 2013 Mar 28;153(1):17-37
pubmed: 23540688
Mol Cancer Res. 2012 Apr;10(4):504-15
pubmed: 22343615
Cancers (Basel). 2022 Mar 25;14(7):
pubmed: 35406446
Carcinogenesis. 2011 Nov;32(11):1641-7
pubmed: 21890460
Aging (Albany NY). 2021 Sep 21;13(18):21991-22029
pubmed: 34547721
J Cell Physiol. 2019 Aug;234(10):17959-17974
pubmed: 30847933
Bioengineered. 2021 Dec;12(1):5830-5838
pubmed: 34488538
J Cell Physiol. 2019 Sep;234(9):14574-14580
pubmed: 30684274
Nature. 1992 Sep 17;359(6392):235-7
pubmed: 1528264
Hum Mol Genet. 2007 Apr 15;16 Spec No 1:R14-20
pubmed: 17613544
Bioinformatics. 2005 Oct 1;21(19):3787-93
pubmed: 15817693
Oncogene. 2007 Nov 29;26(54):7590-5
pubmed: 17563749
Sci Rep. 2016 Dec 5;6(1):1
pubmed: 28442746
Open Biol. 2019 Jan 31;9(1):180212
pubmed: 30958116
Oncol Lett. 2016 Sep;12(3):1687-1692
pubmed: 27588118
Nature. 2012 Jul 18;487(7407):330-7
pubmed: 22810696
PLoS One. 2010 Oct 08;5(10):e13247
pubmed: 20949044
Int Arch Allergy Immunol. 2020;181(4):239-248
pubmed: 31955160
Dis Model Mech. 2017 Mar 1;10(3):197-214
pubmed: 28250048
Cell Death Dis. 2012 Nov 15;3:e423
pubmed: 23152059
Oncol Rep. 2016 Dec;36(6):3275-3282
pubmed: 27748903
Mol Cancer Ther. 2014 Jul;13(7):1942-51
pubmed: 24785257
Biochem Cell Biol. 2019 Oct;97(5):563-570
pubmed: 30807203
Mol Cell Biol. 2015 Mar;35(6):988-1000
pubmed: 25582198
PLoS One. 2013 Jul 29;8(7):e69963
pubmed: 23922874
J Cell Biochem. 2018 Nov;119(11):8763-8772
pubmed: 30126001
NPJ Precis Oncol. 2018 Jan 8;2(1):1
pubmed: 29872720
Trends Pharmacol Sci. 2012 Jul;33(7):382-93
pubmed: 22613783
Microrna. 2012;1(1):34-9
pubmed: 25048088
RNA. 2011 Feb;17(2):312-26
pubmed: 21177881
Invest Ophthalmol Vis Sci. 2013 Jan 14;54(1):323-32
pubmed: 23221074
Int J Mol Sci. 2022 Apr 27;23(9):
pubmed: 35563246
Bioinformatics. 2015 Oct 15;31(20):3377-9
pubmed: 26099263
Cancer Epidemiol. 2012 Feb;36(1):e61-7
pubmed: 22018950
Biol Open. 2016 May 15;5(5):563-70
pubmed: 27095441

Auteurs

Tahani Bakhsh (T)

Department of Biology, College of Science, University of Jeddah, Jeddah, Saudi Arabia. tabakhsh@uj.edu.sa.

Safiah Alhazmi (S)

Department of Biological Sciences, Faculty of Science, King Abdulaziz University, 21589, Jeddah, Saudi Arabia.
Immunology Unit, King Fahad Medical Research Centre, King Abdulaziz University, 80200, Jedaah, Saudi Arabia.
Neuroscience and Geroscience Research Unit, King Fahad Medical Research Centre, King Abdulaziz University, 80200, Jeddah, Saudi Arabia.
Central lab of biological Sciences, Faculty of Sciences, King Abdulaziz University, 80200, Jeddah, Saudi Arabia.

Ali Farsi (A)

Department of Surgery, Faculty of Medicine, King Abdulaziz University, 21589, Jeddah, Saudi Arabia.

Abdulaziz S Yusuf (AS)

Department of Biochemistry, Faculty of science, Stem Cell Unit, King Fahad Center for Medical Research, King Abdulaziz University, 21589, Jeddah, Saudi Arabia.
Medical Laboratory Sciences Department, Fakeeh College for Medical Sciences, 21461, Jeddah, Saudi Arabia.

Amani Alharthi (A)

Department of Biology, College of Science Al-Zulfi, Majmaah University, 11952, Majmaah, Saudi Arabia.

Safa H Qahl (SH)

Department of Biology, College of Science, University of Jeddah, Jeddah, Saudi Arabia.

Maha Ali Alghamdi (MA)

Department of Biotechnology, College of Science, Taif University, Taif, Saudi Arabia.

Faisal A Alzahrani (FA)

Department of Biochemistry, Faculty of science, Stem Cell Unit, King Fahad Center for Medical Research, King Abdulaziz University, 21589, Jeddah, Saudi Arabia.

Ola H Elgaddar (OH)

Department of Chemical Pathology, Alexandria University, Alexandria, Egypt.

Mohanad A Ibrahim (MA)

Data Science Program, King Abdullah International Medical Research Center, 11481, Riyadh, Saudi Arabia.

Ahmed Bahieldin (A)

Department of Biological Sciences, Faculty of Science, King Abdulaziz University, 21589, Jeddah, Saudi Arabia. abmahmed@kau.edu.sa.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Effectiveness of Virtual Yoga for Chronic Low Back Pain: A Randomized Clinical Trial.

Hallie Tankha, Devyn Gaskins, Amanda Shallcross et al.
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH

Recherchemedicale.com › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Molecular detection of exosomal miRNAs of...
Recherchemedicale.com › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › microARN › Molecular detection of exosomal miRNAs of...
Recherchemedicale.com › Systèmes sanguin et immunitaire › Sang › Sérum › Molecular detection of exosomal miRNAs of...
Recherchemedicale.com › Maladies de l'appareil digestif › Maladies gastro-intestinales › Maladies intestinales › Maladies du rectum › Tumeurs colorectales › Molecular detection of exosomal miRNAs of...
Recherchemedicale.com › Diagnostic › Pronostic › Molecular detection of exosomal miRNAs of...
Recherchemedicale.com › Facteurs biologiques › Marqueurs biologiques › Molecular detection of exosomal miRNAs of...
Recherchemedicale.com › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Vésicules cytoplasmiques › Vésicules de transport › Exosomes › Molecular detection of exosomal miRNAs of...