Femtosecond pulsed laser photodynamic therapy activates melanin and eradicates malignant melanoma.

melanin melanoma multiphoton ocular melanoma photodynamic therapy

Journal

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876

Informations de publication

Date de publication:
02 Apr 2024
Historique:
medline: 29 3 2024
pubmed: 29 3 2024
entrez: 29 3 2024
Statut: ppublish

Résumé

Photodynamic therapy (PDT) relies on a series of photophysical and photochemical reactions leading to cell death. While effective for various cancers, PDT has been less successful in treating pigmented melanoma due to high light absorption by melanin. Here, this limitation is addressed by 2-photon excitation of the photosensitizer (2p-PDT) using ~100 fs pulses of near-infrared laser light. A critical role of melanin in enabling rather than hindering 2p-PDT is elucidated using pigmented and non-pigmented murine melanoma clonal cell lines in vitro. The photocytotoxicities were compared between a clinical photosensitizer (Visudyne) and a porphyrin dimer (Oxdime) with ~600-fold higher σ

Identifiants

DOI: 10.1073/pnas.2316303121 PMID: 38551838
pubmed: 38551838
doi: 10.1073/pnas.2316303121
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2316303121

Subventions

Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
ID : 2013-07276-1
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
ID : 305795/2016-3
Organisme : CNPq | Ciência sem Fronteiras (CsF)
ID : N/A
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
ID : N/A
Organisme : Universidade de Sao Paulo - University of Toronto Joint Program
ID : N/A
Organisme : Banting Fellowship from the Canadian Institutes of Health Research
ID : N/A
Organisme : Princess Margaret Cancer Centre Foundation Invest-in-Research Fund
ID : N/A
Organisme : Princess Margaret Cancer Centre Foundation Excellence Fellowship Award
ID : N/A
Organisme : Henry Farrugia Research Fund
ID : N/A
Organisme : Vision Science Research Program Award
ID : N/A
Organisme : Cancer Prevention and Research Institute of Texas (CPRIT)
ID : M20301556
Organisme : Governor's University Research Initiative
ID : M230930

Déclaration de conflit d'intérêts

Competing interests statement:The authors declare no competing interest.

Auteurs

Layla Pires (L)

Department of Cancer Biology and Imaging, Princess Margaret Cancer Center, University Health Network, Toronto, ON M5G 1L7, Canada.
Departamento de Fisica e Ciencia dos Materiais, São Carlos Institute of Physics, University of São Paulo, Sao Carlos 13566-590, Brazil.

Shireen Khattak (S)

Departments of Ophthalmology & Vision Sciences, St. Michael's Hospital, University of Toronto, Toronto, ON M5B 1W8, Canada.

Sebastiao Pratavieira (S)

Departamento de Fisica e Ciencia dos Materiais, São Carlos Institute of Physics, University of São Paulo, Sao Carlos 13566-590, Brazil.

Carla Calcada (C)

Department of Cancer Biology and Imaging, Princess Margaret Cancer Center, University Health Network, Toronto, ON M5G 1L7, Canada.

Renan Romano (R)

Departamento de Fisica e Ciencia dos Materiais, São Carlos Institute of Physics, University of São Paulo, Sao Carlos 13566-590, Brazil.

Yeni Yucel (Y)

Departments of Ophthalmology & Vision Sciences, St. Michael's Hospital, University of Toronto, Toronto, ON M5B 1W8, Canada.
Faculty of Medicine, Department of Ophthalmology, Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, BC V5Z 3N9, Canada.

Vanderlei S Bagnato (VS)

Departamento de Fisica e Ciencia dos Materiais, São Carlos Institute of Physics, University of São Paulo, Sao Carlos 13566-590, Brazil.
Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843.
ORCID: 0000-0003-4833-239X

Cristina Kurachi (C)

Departamento de Fisica e Ciencia dos Materiais, São Carlos Institute of Physics, University of São Paulo, Sao Carlos 13566-590, Brazil.
ORCID: 0000-0001-7175-5337

Brian C Wilson (BC)

Department of Cancer Biology and Imaging, Princess Margaret Cancer Center, University Health Network, Toronto, ON M5G 1L7, Canada.
Faculty of Medicine, Department of Medical Biophysics, University of Toronto, Toronto, ON M5G 1L7, Canada.

Classifications MeSH