Detection of

Aeromonas hydrophila PCR assay aerA gene minimum detectable limit

Journal

Journal of advanced veterinary and animal research
ISSN: 2311-7710
Titre abrégé: J Adv Vet Anim Res
Pays: Bangladesh
ID NLM: 101647585

Informations de publication

Date de publication:
Dec 2023
Historique:
received: 02 06 2023
revised: 22 08 2023
accepted: 20 10 2023
medline: 19 2 2024
pubmed: 19 2 2024
entrez: 19 2 2024
Statut: epublish

Résumé

The aerolysin (aerA) is a virulence indicator used to identify the pathogenicity of the Kidney samples among both healthy and sick Nile tilapias in five provinces of Luzon Island were collected for bacterial analysis. Screening using specific primers targeting The AuNP probe demonstrated a computed accuracy of 81.32%, sensitivity of 100%, and specificity of 81.26%. Among the 257 reactions, 59 were false positives, while no false negative results were observed. The AuNP probe could detect The established colorimetric detection method for

Identifiants

pubmed: 38370905
doi: 10.5455/javar.2023.j714
pmc: PMC10868686
doi:

Types de publication

Journal Article

Langues

eng

Pagination

593-598

Informations de copyright

Copyright: © Journal of Advanced Veterinary and Animal Research.

Déclaration de conflit d'intérêts

The authors assert that there is no conflict of interest.

Auteurs

Arren Christian M de Guia (ACM)

Livestock Biotechnology Center, Philippine Carabao Center, Science City of Muñoz, The Philippines.
Freshwater Aquaculture Center-College of Fisheries, Central Luzon State University, Science City of Muñoz, The Philippines.

Mary Rose D Uy-de Guia (MRDU)

Production System and Nutrition Section, Philippine Carabao Center, Science City of Muñoz, The Philippines.

Juvy J Monserate (JJ)

Department of Chemistry, College of Science, Central Luzon State University, Science City of Muñoz, The Philippines.

Joel R Salazar (JR)

Department of Chemistry, College of Science, Central Luzon State University, Science City of Muñoz, The Philippines.

Ravelina R Velasco (RR)

Freshwater Aquaculture Center-College of Fisheries, Central Luzon State University, Science City of Muñoz, The Philippines.

Claro N Mingala (CN)

Livestock Biotechnology Center, Philippine Carabao Center, Science City of Muñoz, The Philippines.
Department of Animal Science, College of Agriculture, Institute of Graduate Studies, Central Luzon State University, Science City of Muñoz, The Philippines.

Karl Marx A Quiazon (KMA)

Freshwater Aquaculture Center-College of Fisheries, Central Luzon State University, Science City of Muñoz, The Philippines.

Classifications MeSH