IgE to cross-reactive carbohydrate determinants (CCD) in childhood: Prevalence, risk factors, putative origins.

CCD IgE allergens and epitopes allergic rhinitis grass pollen pediatrics

Journal

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
ISSN: 1365-2222
Titre abrégé: Clin Exp Allergy
Pays: England
ID NLM: 8906443

Informations de publication

Date de publication:
17 Jan 2024
Historique:
revised: 08 11 2023
received: 13 02 2023
accepted: 28 11 2023
medline: 18 1 2024
pubmed: 18 1 2024
entrez: 18 1 2024
Statut: aheadofprint

Résumé

IgE antibodies to cross-reactive carbohydrate determinants (CCD) are usually clinically irrelevant but they can be a cause of false positive outcomes of allergen-specific IgE tests in vitro. Their prevalence and levels have been so far cross-sectionally examined among adult allergic patients and much less is known about their origins and relevance in childhood. We examined CCD with a cross-sectional approach in 1263 Italian pollen allergic children (Panallergen in Paediatrics, PAN-PED), as well as with a longitudinal approach in 612 German children (Multicenter Allergy Study, MAS), whose cutaneous and IgE sensitization profile to a broad panel of allergen extracts and molecules was already known. The presence and levels of IgE to CCD were examined in the sera of both cohorts using bromelain (MUXF3) as reagent and a novel chemiluminescence detection system, operating in a solid phase of fluorescently labelled and streptavidin-coated paramagnetic microparticles (NOVEOS, HYCOR, USA). IgE to CCD was found in 22% of the Italian pollen allergic children, mainly in association with an IgE response to grass pollen. Children with IgE to CCD had higher total IgE levels and were sensitized to more allergenic molecules of Phleum pratense than those with no IgE to CCD. Among participants of the German MAS birth cohort study, IgE to CCD emerged early in life (even at pre-school age), with IgE sensitization to group 1 and 4 allergen molecules of grasses, and almost invariably persisted over the full observation period. Our results contribute to dissect the immunological origins, onset, evolution and risk factors of CCD-sIgE response in childhood, and raise the hypothesis that group 1 and/or 4 allergen molecules of grass pollen are major inducers of these antibodies through an antigen-specific, T-B cell cognate interaction.

Sections du résumé

BACKGROUND BACKGROUND
IgE antibodies to cross-reactive carbohydrate determinants (CCD) are usually clinically irrelevant but they can be a cause of false positive outcomes of allergen-specific IgE tests in vitro. Their prevalence and levels have been so far cross-sectionally examined among adult allergic patients and much less is known about their origins and relevance in childhood.
METHODS METHODS
We examined CCD with a cross-sectional approach in 1263 Italian pollen allergic children (Panallergen in Paediatrics, PAN-PED), as well as with a longitudinal approach in 612 German children (Multicenter Allergy Study, MAS), whose cutaneous and IgE sensitization profile to a broad panel of allergen extracts and molecules was already known. The presence and levels of IgE to CCD were examined in the sera of both cohorts using bromelain (MUXF3) as reagent and a novel chemiluminescence detection system, operating in a solid phase of fluorescently labelled and streptavidin-coated paramagnetic microparticles (NOVEOS, HYCOR, USA).
RESULTS RESULTS
IgE to CCD was found in 22% of the Italian pollen allergic children, mainly in association with an IgE response to grass pollen. Children with IgE to CCD had higher total IgE levels and were sensitized to more allergenic molecules of Phleum pratense than those with no IgE to CCD. Among participants of the German MAS birth cohort study, IgE to CCD emerged early in life (even at pre-school age), with IgE sensitization to group 1 and 4 allergen molecules of grasses, and almost invariably persisted over the full observation period.
CONCLUSIONS CONCLUSIONS
Our results contribute to dissect the immunological origins, onset, evolution and risk factors of CCD-sIgE response in childhood, and raise the hypothesis that group 1 and/or 4 allergen molecules of grass pollen are major inducers of these antibodies through an antigen-specific, T-B cell cognate interaction.

Identifiants

DOI: 10.1111/cea.14439 PMID: 38234127
pubmed: 38234127
doi: 10.1111/cea.14439
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Hycor BiomedicalDeutsche Forschungs Gesellschaft
ID : MA 4720/2
Organisme : German Federal Ministry of Education and Research
ID : 07015633, 07 ALE 27, 01EE9405/5, 01EE9406
Organisme : German Research Foundation
ID : KE 1462/2-1, and MA-4740/1

Informations de copyright

Clinical & Experimental Allergy© 2023 The Authors. Clinical & Experimental Allergy published by John Wiley & Sons Ltd.

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Auteurs

Ekaterina Potapova (E)

Department of Pediatric Respiratory Medicine, Immunology and Critical Care Medicine, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany.
ORCID: 0000-0003-4427-6335

Salvatore Tripodi (S)

Pediatric Department and Pediatric Allergology Unit, Sandro Pertini Hospital, Rome, Italy.

Valentina Panetta (V)

Consultancy & Training, Biostatistics, Rome, Italy.

Stephanie Dramburg (S)

Department of Pediatric Respiratory Medicine, Immunology and Critical Care Medicine, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany.
ORCID: 0000-0002-9303-3260

Roberto Bernardini (R)

Pediatric Unit, San Giuseppe Hospital, Empoli, Italy.

Carlo Caffarelli (C)

Department of Medicine and Surgery, Clinica Pediatrica, University of Parma, Parma, Italy.

Antonella Casani (A)

Pediatra di Libera Scelta, Benevento, Italy.

Rosa Cervone (R)

Pediatric Unit, San Giuseppe Hospital, Empoli, Italy.

Loredana Chini (L)

UOSD di Immunopatologia ed Allergologia Pediatrica, Policlinico Tor Vergata, Università di Roma Tor Vergata, Rome, Italy.

Pasquale Comberiati (P)

Department of Clinical and Experimental Medicine, Section of Paediatrics, University of Pisa, Pisa, Italy.
ORCID: 0000-0001-5209-9733

Giovanna De Castro (G)

Pediatric Department, La Sapienza University, Rome, Italy.

Michele Miraglia Del Giudice (MM)

Dipartimento della Donna, del Bambino e di Chirurgia Generale e Specialistica, Università della Campania Luigi Vanvitelli, Naples, Italy.

Iride Dello Iacono (I)

Pediatric Unit, Fatebenefratelli Hospital, Benevento, Italy.

Andrea Di Rienzo Businco (A)

Pediatric Department and Pediatric Allergology Unit, Sandro Pertini Hospital, Rome, Italy.

Marcella Gallucci (M)

Pediatric Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy.

Arianna Giannetti (A)

Pediatric Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy.

Viviana Moschese (V)

UOSD di Immunopatologia ed Allergologia Pediatrica, Policlinico Tor Vergata, Università di Roma Tor Vergata, Rome, Italy.

Ifigenia Sfika (I)

Pediatric Department and Pediatric Allergology Unit, Sandro Pertini Hospital, Rome, Italy.

Elena Varin (E)

Pediatric Intermediate Care Unit, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.

Riccardo Asero (R)

Allergology Service, San Carlo Clinic, Milan, Paderno Dugnano, Italy.

Annamaria Bianchi (A)

Pediatric Unit, San Camillo Forlanini Hospital, Rome, Italy.

Mauro Calvani (M)

Pediatric Unit, San Camillo Forlanini Hospital, Rome, Italy.

Tullio Frediani (T)

Pediatric Department, La Sapienza University, Rome, Italy.

Francesco Macrì (F)

Pediatric Department, La Sapienza University, Rome, Italy.

Nunzia Maiello (N)

Dipartimento della Donna, del Bambino e di Chirurgia Generale e Specialistica, Università della Campania Luigi Vanvitelli, Naples, Italy.

Francesco Paravati (F)

Pediatric Unit, Crotone, Italy.

Umberto Pelosi (U)

Pediatric Unit, Santa Barbara Hospital, Iglesias, Italy.

Diego Peroni (D)

Department of Life and Reproduction Sciences, Pediatric Section, University of Verona, Verona, Italy.

Giuseppe Pingitore (G)

Pediatric Unit, Grassi Hospital, Rome, Italy.

Mariangela Tosca (M)

Pulmonary Disease and Allergy Unit, G. Gaslini Hospital, Genoa, Italy.

Anna Maria Zicari (AM)

Pediatric Department, La Sapienza University, Rome, Italy.

Giampaolo Ricci (G)

Pediatric Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy.

Gerald Reese (G)

Research and Development Division, Allergopharma Joachim Ganzer KG, Reinbek, Germany.

Linus Grabenhenrich (L)

Institute of Social Medicine, Epidemiology and Health Economics, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany.

Katja Icke (K)

Institute of Social Medicine, Epidemiology and Health Economics, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany.

Armin Grübl (A)

Department of Pediatrics, Technical University of Munich, Munich, Germany.

Christoph Müller (C)

Center for Pediatrics and Adolescent Medicine, University Medical Centre and Faculty of Medicine Freiburg, Freiburg im Breisgau, Germany.

Fred Zepp (F)

Department of Pediatrics and Adolescent Medicine, University Medicine Mainz, Mainz, Germany.

Antje Schuster (A)

Department of Pediatrics, Heinrich-Heine-University, Düsseldorf, Germany.

Ulrich Wahn (U)

Department of Pediatric Respiratory Medicine and Immunology, Former director of Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany.

Susanne Lau (S)

Department of Pediatric Respiratory Medicine, Immunology and Critical Care Medicine, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany.

Thomas Keil (T)

Institute of Social Medicine, Epidemiology and Health Economics, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany.
Institute of Clinical Epidemiology and Biometry, University of Würzburg, Würzburg, Germany.
State Institute of Health, Bavarian Health and Food Safety Authority, Erlangen, Germany.

Paolo Maria Matricardi (PM)

Department of Pediatric Respiratory Medicine, Immunology and Critical Care Medicine, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany.
ORCID: 0000-0001-5485-0324

Classifications MeSH