Improvement of the qmosRT-PCR Assay and Its Application for the Detection and Quantitation of the Three Serotypes of the Novel Oral Polio Vaccine in Stool Samples.
OPV
clinical samples
nOPV
poliovirus
vaccines
virus excretion
Journal
Vaccines
ISSN: 2076-393X
Titre abrégé: Vaccines (Basel)
Pays: Switzerland
ID NLM: 101629355
Informations de publication
Date de publication:
19 Nov 2023
19 Nov 2023
Historique:
received:
06
10
2023
revised:
09
11
2023
accepted:
10
11
2023
medline:
25
11
2023
pubmed:
25
11
2023
entrez:
25
11
2023
Statut:
epublish
Résumé
Recently, genetically stable novel OPVs (nOPV) were developed by modifying the genomes of Sabin viruses of conventional OPVs to reduce the risk of reversion to neurovirulence and therefore the risk of generating circulating vaccine-derived polioviruses. There is a need for specific and sensitive methods for the identification and quantification of nOPV viruses individually and in mixtures for clinical trials and potentially for manufacturing quality control and environmental surveillance. In this communication, we evaluated and improved the quantitative multiplex one-step reverse transcriptase polymerase chain reaction (qmosRT-PCR) assay for the identification and quantification of nOPV viruses in samples with different formulations and virus concentrations and in virus-spiked stool samples. The assay was able to specifically identify at least 1 log
Identifiants
pubmed: 38006061
pii: vaccines11111729
doi: 10.3390/vaccines11111729
pmc: PMC10675353
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Bill & Melinda Gates Foundation
ID : OPP1150001
Pays : United States
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