A semi-automated microscopic image analysis method for scoring Ki-67 nuclear immunostaining.

Immunohistochemistry Ki-67 Antigen / metabolism

Journal

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas
ISSN: 1414-431X
Titre abrégé: Braz J Med Biol Res
Pays: Brazil
ID NLM: 8112917

Informations de publication

Date de publication:
2023
Historique:
received: 12 07 2023
accepted: 11 09 2023
medline: 20 11 2023
pubmed: 17 11 2023
entrez: 16 11 2023
Statut: epublish

Résumé

Nuclear proliferation marker MIB-1 (Ki-67) immunohistochemistry (IHC) is used to examine tumor cell proliferation. However, the diagnostic or prognostic value of the Ki-67 nuclear staining intensity and location, defined as nuclear gradient (NG), has not been assessed. This study examined the potential association between Ki-67 NG and cell cycle phases and its effect on the prognosis of pulmonary typical carcinoid (PTC) tumors. We propose a method for classifying the NG of Ki-67 during the cell cycle and compare the results between PTC, pulmonary adenocarcinoma (PAD), and breast ductal carcinoma (BDC). A literature review and objective analysis of IHC-stained paraffin sections were used to determine the Ki-67 labeling index and composed a stratification of the NG into NG1, NG2, and NG3/4 categories. A semi-automated image analysis protocol was established to determine the Ki-67 NG in PTC, PAD, and BDC. High intraobserver consistency and moderate interobserver agreement were achieved in the determination of Ki-67 NG in tumor specimens. NG1 and NG2 were lower in PTC than in PAD and BDC. Cox multivariate analysis of PTC after adjusting for age and number of metastatic lymph nodes showed that Ki-67 NG1 and NG2 significantly predicted clinical outcomes. The semi-automated method for quantification of Ki-67 nuclear immunostaining proposed in this study could become a valuable diagnostic and prognostic tool in PTC.

Identifiants

DOI: 10.1590/1414-431X2023e12922 PMID: 37970922 PMC: PMC10644968
pubmed: 37970922
pii: S0100-879X2023000100689
doi: 10.1590/1414-431X2023e12922
pmc: PMC10644968
pii:
doi:

Substances chimiques

Ki-67 Antigen 0

Types de publication

Review Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e12922

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Auteurs

S M Fernezlian (SM)

Laboratório de Genômica e Histomorfometria, Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil.
ORCID: 0000-0002-2551-461X

C M Baldavira (CM)

Laboratório de Genômica e Histomorfometria, Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil.
ORCID: 0000-0002-5364-7305

M L F de Souza (MLF)

Laboratório de Genômica e Histomorfometria, Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil.
ORCID: 0000-0002-7220-5852

C Farhat (C)

Laboratório de Genômica e Histomorfometria, Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil.
ORCID: 0000-0002-8047-0082

A F de Vilhena (AF)

Departamento de Cirurgia Torácica, Instituto do Coração, São Paulo, SP, Brasil.
ORCID: 0000-0002-7307-3046

J C N Pereira (JCN)

Department of General Thoracic Surgery, Georges Pompidou European Hospital, Paris, France.
International Perioperative Europrogram, Paris, France.
ORCID: 0000-0002-3553-7006

J R M de Campos (JRM)

Departamento de Cirurgia Torácica, Instituto do Coração, São Paulo, SP, Brasil.
Departamento de Cirurgia Torácica, Hospital Israelita Albert Einstein, São Paulo, SP, Brasil.
ORCID: 0000-0002-2385-7707

T Takagaki (T)

Divisão de Pneumologia, Instituto do Coração, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil.
ORCID: 0000-0003-2277-2100

M L Balancin (ML)

Laboratório de Genômica e Histomorfometria, Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil.
ORCID: 0000-0003-3798-8414

A M Ab'Saber (AM)

Laboratório de Genômica e Histomorfometria, Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil.
ORCID: 0000-0002-6371-177X

V L Capelozzi (VL)

Laboratório de Genômica e Histomorfometria, Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil.
ORCID: 0000-0001-9732-5853

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Classifications MeSH

Recherchemedicale.com › Disciplines des sciences naturelles › Chimie › Biochimie › Immunohistochimie › A semi-automated microscopic image analysis...
Recherchemedicale.com › Facteurs biologiques › Marqueurs biologiques › Marqueurs biologiques tumoraux › Antigène KI-67 › A semi-automated microscopic image analysis...