MePMe-seq: antibody-free simultaneous m
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
07 11 2023
07 11 2023
Historique:
received:
04
03
2022
accepted:
23
10
2023
medline:
9
11
2023
pubmed:
8
11
2023
entrez:
7
11
2023
Statut:
epublish
Résumé
Internal modifications of mRNA have emerged as widespread and versatile regulatory mechanism to control gene expression at the post-transcriptional level. Most of these modifications are methyl groups, making S-adenosyl-L-methionine (SAM) a central metabolic hub. Here we show that metabolic labeling with a clickable metabolic precursor of SAM, propargyl-selenohomocysteine (PSH), enables detection and identification of various methylation sites. Propargylated A, C, and G nucleosides form at detectable amounts via intracellular generation of the corresponding SAM analogue. Integration into next generation sequencing enables mapping of N
Identifiants
pubmed: 37935679
doi: 10.1038/s41467-023-42832-z
pii: 10.1038/s41467-023-42832-z
pmc: PMC10630376
doi:
Substances chimiques
RNA, Messenger
0
RNA
63231-63-0
S-Adenosylmethionine
7LP2MPO46S
Antibodies
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7154Informations de copyright
© 2023. The Author(s).
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