Phyloproteomic study by MALDI-TOF MS in view of intraspecies variation in a significant homogenous phytopathogen Dickeya solani.

Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods Enterobacteriaceae / genetics Dickeya Gammaproteobacteria

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
01 11 2023
Historique:
received: 29 07 2023
accepted: 26 10 2023
medline: 3 11 2023
pubmed: 2 11 2023
entrez: 2 11 2023
Statut: epublish

Résumé

Dickeya solani is an economically significant pectinolytic phytopathogen belonging to the Pectobacteriaceae family, which causes soft rot and blackleg diseases. Despite its notable impact on global potato production, there are no effective methods to control this pest. Here, we undertook a phyloproteomic study on 20 D. solani strains, of various origin and year of isolation, with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) supported by an in-depth characterization of the strains in terms of the virulence-associated phenotype. In spite of high homogeneity in this species, we herein revealed for the first time intraspecies variation in the MALDI-TOF MS protein profiles among the studied D. solani isolates. Finally, representative mass spectra for the four delineated clades are presented. A majority of the analysed D. solani strains showed high virulence potential, while two strains stood out in their growth dynamics, virulence factors production and ability to macerate plant tissue. Nonetheless, the metabolic profiles of D. solani strains turned out to be uniform, except for gelatinase activity. Given that all D. solani isolates distinctly grouped from the other Dickeya species in the MALDI-TOF MS analysis, there is strong evidence supporting the potential routine use of this method for fast and reliable to-species identification of D. solani isolates of environmental origin.

Identifiants

DOI: 10.1038/s41598-023-46012-3 PMID: 37914755 PMC: PMC10620192
pubmed: 37914755
doi: 10.1038/s41598-023-46012-3
pii: 10.1038/s41598-023-46012-3
pmc: PMC10620192
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

18863

Informations de copyright

© 2023. The Author(s).

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Auteurs

Agata Motyka-Pomagruk (A)

Laboratory of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of Gdansk, University of Gdansk, 58 Abrahama, 80-307, Gdańsk, Poland.
Research & Development Laboratory, Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of Gdansk, University of Gdansk, 20 Podwale Przedmiejskie, 80-824, Gdańsk, Poland.

Weronika Babinska-Wensierska (W)

Laboratory of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of Gdansk, University of Gdansk, 58 Abrahama, 80-307, Gdańsk, Poland.
Research & Development Laboratory, Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of Gdansk, University of Gdansk, 20 Podwale Przedmiejskie, 80-824, Gdańsk, Poland.

Wojciech Sledz (W)

Laboratory of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of Gdansk, University of Gdansk, 58 Abrahama, 80-307, Gdańsk, Poland.
Research & Development Laboratory, Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of Gdansk, University of Gdansk, 20 Podwale Przedmiejskie, 80-824, Gdańsk, Poland.

Anna-Karina Kaczorowska (AK)

Collection of Plasmids and Microorganisms (KPD), Faculty of Biology, University of Gdansk, 59 Wita Stwosza Street, 80-308, Gdańsk, Poland.

Ewa Lojkowska (E)

Laboratory of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of Gdansk, University of Gdansk, 58 Abrahama, 80-307, Gdańsk, Poland. ewa.lojkowska@biotech.ug.edu.pl.
Research & Development Laboratory, Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of Gdansk, University of Gdansk, 20 Podwale Przedmiejskie, 80-824, Gdańsk, Poland. ewa.lojkowska@biotech.ug.edu.pl.

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Classifications MeSH

Recherchemedicale.com › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse MALDI › Phyloproteomic study by MALDI-TOF MS in view...
Recherchemedicale.com › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Phyloproteomic study by MALDI-TOF MS in view...
Recherchemedicale.com › Bactéries › Proteobacteria › Gammaproteobacteria › Dickeya › Phyloproteomic study by MALDI-TOF MS in view...
Recherchemedicale.com › Bactéries › Proteobacteria › Gammaproteobacteria › Phyloproteomic study by MALDI-TOF MS in view...