Depolarized Mitochondrial Membrane Potential and Elevated Calcium in Platelets of Sickle Cell Disease.
Calcium content
Mitochondrial membrane potential
Platelet activation
Sickle cell disease
Surface markers
Journal
Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion
ISSN: 0971-4502
Titre abrégé: Indian J Hematol Blood Transfus
Pays: India
ID NLM: 9425818
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
received:
23
12
2022
accepted:
23
02
2023
pmc-release:
01
10
2024
medline:
3
10
2023
pubmed:
3
10
2023
entrez:
3
10
2023
Statut:
ppublish
Résumé
Hemolysis, a crucial feature of Sickle cell disease (SCD), is a key player for cellular activation leading to various complications including thrombosis. In response to hemolysis, platelets get activated and release components that are necessary for further platelet activation and aggregation. Thus, it is believed that platelets contribute to the development of thrombotic complications. Platelets in SCD are expected to be affected due to common cause of hemolysis. To measure the surface markers of platelets including P-Selectin, Phosphatidyl Serine and integrin αIIbβ3 in SCD patients and healthy controls in order to understand the status of the platelets in SCD. To measure the surface markers of activated platelets using flow cytometry. Since mitochondria and calcium play an important role in cellular functions, the mitochondrial membrane potential and calcium content of platelets in SCD were also evaluated using flow cytometry. In the present study, we have observed significant increase of calcium level in SCD platelets. Further, the loss of mitochondrial membrane potential in SCD platelets was found to be significantly higher when compared to platelets of healthy controls. Though the surface markers of activated platelets in SCD remain unchanged, increased level of calcium and mitochondrial membrane potential loss suggest that the platelets in SCD are more prone to become activated. In order to understand the status of the platelets in SCD, apart from the surface markers, it is also important to assess the calcium levels and mitochondrial membrane potential of platelets.
Identifiants
pubmed: 37786815
doi: 10.1007/s12288-023-01640-7
pii: 1640
pmc: PMC10542052
doi:
Types de publication
Journal Article
Langues
eng
Pagination
565-571Informations de copyright
© The Author(s), under exclusive licence to Indian Society of Hematology and Blood Transfusion 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
Déclaration de conflit d'intérêts
Conflict of interestThe authors have no conflicts of interests.
Références
Mol Med. 2022 May 4;28(1):51
pubmed: 35508969
Am J Clin Pathol. 2008 Jul;130(1):93-8
pubmed: 18550477
Pathology. 2017 Jan;49(1):1-9
pubmed: 27914684
Br J Haematol. 1998 Mar;100(4):741-9
pubmed: 9531343
Blood. 2014 May 1;123(18):2864-72
pubmed: 24677541
J Thromb Haemost. 2021 Sep;19(9):2256-2262
pubmed: 33724688
Int J Mol Sci. 2021 Dec 30;23(1):
pubmed: 35008814
Platelets. 2014;25(5):373-81
pubmed: 23909816
J Clin Invest. 1984 Mar;73(3):626-32
pubmed: 6231306
Br J Haematol. 2018 Mar;180(5):761-767
pubmed: 29383704
Am J Hematol. 1992 Aug;40(4):290-4
pubmed: 1503084
Platelets. 2014;25(1):27-35
pubmed: 23469943
J Trauma Acute Care Surg. 2013 Feb;74(2):411-7; discussion 418
pubmed: 23354232
J Lab Clin Med. 2001 Jun;137(6):398-407
pubmed: 11385360
JAMA. 2022 Jul 5;328(1):57-68
pubmed: 35788790
Blood Rev. 2019 Sep;37:100583
pubmed: 31133440
Physiol Res. 2021 Dec 31;70(S3):S369-S379
pubmed: 35099255
Crit Care. 2014 Feb 12;18(1):R31
pubmed: 24521521
J Thromb Haemost. 2014 Dec;12(12):2010-6
pubmed: 25280124
Blood. 2007 Sep 15;110(6):2166-72
pubmed: 17536019
Trends Cardiovasc Med. 2008 Feb;18(2):57-61
pubmed: 18308196
Platelets. 2015;26(5):474-9
pubmed: 25140584
Blood. 2022 Jul 14;140(2):121-139
pubmed: 35472164
Am J Hematol. 2019 Aug;94(8):862-870
pubmed: 31074115
J Thromb Haemost. 2009 Nov;7(11):1878-85
pubmed: 19691478
Exp Biol Med (Maywood). 2016 Apr;241(7):679-88
pubmed: 27013545
J Thromb Haemost. 2009 Jul;7(7):1057-66
pubmed: 19422456
Lancet. 2010 Dec 11;376(9757):2018-31
pubmed: 21131035
Blood. 2008 Feb 1;111(3):1257-65
pubmed: 17989312