Molecular basis for bacterial N-glycosylation by a soluble HMW1C-like N-glycosyltransferase.

Glycosylation Bacterial Proteins / genetics Asparagine Haemophilus influenzae Glucose Uridine Diphosphate

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
18 09 2023
Historique:
received: 18 05 2023
accepted: 24 08 2023
medline: 20 9 2023
pubmed: 19 9 2023
entrez: 18 9 2023
Statut: epublish

Résumé

Soluble HMW1C-like N-glycosyltransferases (NGTs) catalyze the glycosylation of Asn residues in proteins, a process fundamental for bacterial autoaggregation, adhesion and pathogenicity. However, our understanding of their molecular mechanisms is hindered by the lack of structures of enzymatic complexes. Here, we report structures of binary and ternary NGT complexes of Aggregatibacter aphrophilus NGT (AaNGT), revealing an essential dyad of basic/acidic residues located in the N-terminal all α-domain (AAD) that intimately recognizes the Thr residue within the conserved motif Asn

Identifiants

DOI: 10.1038/s41467-023-41238-1 PMID: 37723184 PMC: PMC10507012
pubmed: 37723184
doi: 10.1038/s41467-023-41238-1
pii: 10.1038/s41467-023-41238-1
pmc: PMC10507012
doi:

Substances chimiques

N-glycosyltransferase, Nocardia aerocolonigenes EC 2.4.1.-
Bacterial Proteins 0
Asparagine 7006-34-0
Glucose IY9XDZ35W2
Uridine Diphosphate 58-98-0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

5785

Informations de copyright

© 2023. Springer Nature Limited.

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Auteurs

Beatriz Piniello (B)

Departament de Química Inorgànica i Orgànica (Secció de Química Orgànica) and Institut de Química Teòrica i Computacional (IQTCUB), Universitat de Barcelona, Martí i Franquès 1, 08028, Barcelona, Spain.

Javier Macías-León (J)

Institute of Biocomputation and Physics of Complex Systems, University of Zaragoza, Mariano Esquillor s/n, Campus Rio Ebro, Edificio I+D, Zaragoza, Spain.

Shun Miyazaki (S)

Department of Life Science and Applied Chemistry, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya, 466-8555, Japan.

Ana García-García (A)

Institute of Biocomputation and Physics of Complex Systems, University of Zaragoza, Mariano Esquillor s/n, Campus Rio Ebro, Edificio I+D, Zaragoza, Spain.
ORCID: 0000-0003-3791-2997

Ismael Compañón (I)

Departamento de Química, Universidad de La Rioja, Centro de Investigación en Síntesis Química, E-26006, Logroño, Spain.

Mattia Ghirardello (M)

Departamento de Química, Universidad de La Rioja, Centro de Investigación en Síntesis Química, E-26006, Logroño, Spain.
ORCID: 0000-0002-2855-4801

Víctor Taleb (V)

Institute of Biocomputation and Physics of Complex Systems, University of Zaragoza, Mariano Esquillor s/n, Campus Rio Ebro, Edificio I+D, Zaragoza, Spain.

Billy Veloz (B)

Institute of Biocomputation and Physics of Complex Systems, University of Zaragoza, Mariano Esquillor s/n, Campus Rio Ebro, Edificio I+D, Zaragoza, Spain.

Francisco Corzana (F)

Departamento de Química, Universidad de La Rioja, Centro de Investigación en Síntesis Química, E-26006, Logroño, Spain.
ORCID: 0000-0001-5597-8127

Atsushi Miyagawa (A)

Department of Life Science and Applied Chemistry, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya, 466-8555, Japan.
ORCID: 0000-0003-0809-0912

Carme Rovira (C)

Departament de Química Inorgànica i Orgànica (Secció de Química Orgànica) and Institut de Química Teòrica i Computacional (IQTCUB), Universitat de Barcelona, Martí i Franquès 1, 08028, Barcelona, Spain. c.rovira@ub.edu.
Institució Catalana de Recerca i Estudis Avançats (ICREA), Passeig Lluís Companys 23, 08010, Barcelona, Spain. c.rovira@ub.edu.
ORCID: 0000-0003-1477-5010

Ramon Hurtado-Guerrero (R)

Institute of Biocomputation and Physics of Complex Systems, University of Zaragoza, Mariano Esquillor s/n, Campus Rio Ebro, Edificio I+D, Zaragoza, Spain. rhurtado@bifi.es.
Copenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark. rhurtado@bifi.es.
Fundación ARAID, 50018, Zaragoza, Spain. rhurtado@bifi.es.
ORCID: 0000-0002-3122-9401

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Classifications MeSH

Recherchemedicale.com › Métabolisme › Métabolisme glucidique › Glycosylation › Molecular basis for bacterial N-glycosylation...
Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Molecular basis for bacterial N-glycosylation...
Recherchemedicale.com › Acides aminés, peptides et protéines › Acides aminés › Acides aminés neutres › Asparagine › Molecular basis for bacterial N-glycosylation...
Recherchemedicale.com › Bactéries › Proteobacteria › Gammaproteobacteria › Pasteurellaceae › Haemophilus › Haemophilus influenzae › Molecular basis for bacterial N-glycosylation...
Recherchemedicale.com › Glucides › Sucres › Oses › Hexose › Glucose › Molecular basis for bacterial N-glycosylation...
Recherchemedicale.com › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Ribonucléotides › Nucléotides uridyliques › Uridine diphosphate › Molecular basis for bacterial N-glycosylation...