Engineering of Therapeutic and Detoxifying Enzymes.

Artificial Metalloenzymes Base Editor Directed Evolution Enzyme Design Enzyme Therapy

Journal

Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543

Informations de publication

Date de publication:
13 11 2023
Historique:
received: 22 06 2023
medline: 8 11 2023
pubmed: 11 7 2023
entrez: 11 7 2023
Statut: ppublish

Résumé

Therapeutic enzymes present excellent opportunities for the treatment of human disease, modulation of metabolic pathways and system detoxification. However, current use of enzyme therapy in the clinic is limited as naturally occurring enzymes are seldom optimal for such applications and require substantial improvement by protein engineering. Engineering strategies such as design and directed evolution that have been successfully implemented for industrial biocatalysis can significantly advance the field of therapeutic enzymes, leading to biocatalysts with new-to-nature therapeutic activities, high selectivity, and suitability for medical applications. This minireview highlights case studies of how state-of-the-art and emerging methods in protein engineering are explored for the generation of therapeutic enzymes and discusses gaps and future opportunities in the field of enzyme therapy.

Identifiants

pubmed: 37433049
doi: 10.1002/anie.202308814
doi:

Substances chimiques

Enzymes 0

Types de publication

Journal Article Review Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e202308814

Informations de copyright

© 2023 Wiley-VCH GmbH.

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Auteurs

Freideriki Michailidou (F)

Department of Health Sciences and Technology, ETH Zurich, Schmelzbergstrasse 9, 8092, Zürich, Switzerland.

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