Surface proteins of Shiga toxin-producing

MFGMPs STEC bacterial anti-adhesion flagellin food

Journal

Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977

Informations de publication

Date de publication:
2023
Historique:
received: 01 02 2023
accepted: 29 05 2023
medline: 10 7 2023
pubmed: 10 7 2023
entrez: 10 7 2023
Statut: epublish

Résumé

By adhering to host cells and colonizing tissues, bacterial pathogens can successfully establish infection. Adhesion is considered the first step of the infection process and bacterial adhesion to anti-adhesive compounds is now seen as a promising strategy to prevent infectious diseases. Among the natural sources of anti-adhesive molecules, the membrane of milk fat globules (MFGs) is of interest because of its compositional diversity of proteins and glycoconjugates. However, few studies have focused on the bacterial molecules involved in MFG- mediated inhibition of bacterial adhesion to enterocytes. We used three pathogenic Shiga toxin-producing Escherichia coli (STEC) strains (O26:H11 str. 21765, O157:H7 str. EDL933, and O103:H3 str. PMK5) as models to evaluate whether STEC surface proteins are involved in the affinity of STEC for MFG membrane proteins (MFGMPs). The affinity of STEC for MFGMPs was assessed both indirectly by a natural raw milk creaming test and directly by an adhesion test. Mass spectrometry was used to identify enriched STEC proteins within the protein fraction of MFGMs. Bacterial mutants were constructed and their affinity to MFGs were measured to confirm the role of the identified proteins. We found that free STEC surface proteins inhibit the concentration of the pathogen in the MFG-enriched cream in a strain-dependent manner. Moreover, the OmpA and FliC proteins were identified within the protein fraction of MFGMs. Our results suggest that FliC protein participates in STEC adhesion to MFGMPs but other STEC molecules may also participate. For the first time, this study highlighted, the involvement of STEC surface proteins in the affinity for MFGs. The mechanism of STEC-MFG association is still not fully understood but our results confirm the existence of receptor/ligand type interactions between the bacteria and MFGs. Further studies are needed to identify and specify the molecules involved in this interaction. These studies should consider the likely involvement of several factors, including adhesion molecules, and the diversity of each STEC strain.

Identifiants

DOI: 10.3389/fmicb.2023.1156374 PMID: 37426002 PMC: PMC10328742
pubmed: 37426002
doi: 10.3389/fmicb.2023.1156374
pmc: PMC10328742
doi:

Types de publication

Journal Article

Langues

eng

Pagination

1156374

Informations de copyright

Copyright © 2023 Bagel, Bouvier-Crozier, Canizares, Hamadou, Courcol, Lopez, Michel, Douellou and Sergentet.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Arthur Bagel (A)

Bacterial Opportunistic Pathogens and Environment Research Group, UMR 5557 Ecologie Microbienne Lyon, CNRS, Université de Lyon, VetAgro Sup, INRAE, Marcy-l'Etoile, France.

Marion Bouvier-Crozier (M)

Bacterial Opportunistic Pathogens and Environment Research Group, UMR 5557 Ecologie Microbienne Lyon, CNRS, Université de Lyon, VetAgro Sup, INRAE, Marcy-l'Etoile, France.
Laboratoire d'Etudes des Microorganismes Alimentaires Pathogènes-French National Reference Laboratory for Escherichia coli Including Shiga Toxin-Producing E. coli (NRL-STEC), Université de Lyon, VetAgro Sup-Campus Vétérinaire, Marcy-l'Etoile, France.

Mélissa Canizares (M)

Laboratoire d'Etudes des Microorganismes Alimentaires Pathogènes-French National Reference Laboratory for Escherichia coli Including Shiga Toxin-Producing E. coli (NRL-STEC), Université de Lyon, VetAgro Sup-Campus Vétérinaire, Marcy-l'Etoile, France.

Badis Hamadou (B)

Laboratoire d'Etudes des Microorganismes Alimentaires Pathogènes-French National Reference Laboratory for Escherichia coli Including Shiga Toxin-Producing E. coli (NRL-STEC), Université de Lyon, VetAgro Sup-Campus Vétérinaire, Marcy-l'Etoile, France.

Louise Courcol (L)

Bacterial Opportunistic Pathogens and Environment Research Group, UMR 5557 Ecologie Microbienne Lyon, CNRS, Université de Lyon, VetAgro Sup, INRAE, Marcy-l'Etoile, France.

Christelle Lopez (C)

INRAE, UR BIA, Nantes, France.

Valérie Michel (V)

Actalia, La Roche-sur-Foron, France.

Thomas Douellou (T)

Bacterial Opportunistic Pathogens and Environment Research Group, UMR 5557 Ecologie Microbienne Lyon, CNRS, Université de Lyon, VetAgro Sup, INRAE, Marcy-l'Etoile, France.

Delphine Sergentet (D)

Bacterial Opportunistic Pathogens and Environment Research Group, UMR 5557 Ecologie Microbienne Lyon, CNRS, Université de Lyon, VetAgro Sup, INRAE, Marcy-l'Etoile, France.
Laboratoire d'Etudes des Microorganismes Alimentaires Pathogènes-French National Reference Laboratory for Escherichia coli Including Shiga Toxin-Producing E. coli (NRL-STEC), Université de Lyon, VetAgro Sup-Campus Vétérinaire, Marcy-l'Etoile, France.

Classifications MeSH