Immunohistochemical detection of MUC5AC and MUC5B mucins in ferrets.
Cystic fibrosis
Ferret
Immunohistochemistry
Lung
MUC5AC
MUC5B
Mucin
Mucus
Pathology
Tissue
Journal
BMC research notes
ISSN: 1756-0500
Titre abrégé: BMC Res Notes
Pays: England
ID NLM: 101462768
Informations de publication
Date de publication:
22 Jun 2023
22 Jun 2023
Historique:
received:
10
01
2023
accepted:
15
06
2023
medline:
26
6
2023
pubmed:
23
6
2023
entrez:
22
6
2023
Statut:
epublish
Résumé
Cystic fibrosis (CF) is a genetic condition that causes abnormal mucus secretions in affected organs. MUC5AC and MUC5B are gel-forming mucins and frequent targets for investigations in CF tissues. Our objective was to qualify MUC5AC and MUC5B immunohistochemical techniques to provide a useful tool to identify, localize and interpret mucin expression in ferret tissues. MUC5AC and MUC5B mucins were detected most commonly in large airways and least in small airways, consistent with reported goblet cell density in airway surface epithelia. We evaluated whether staining method affected the detection of goblet cell mucins in serial sections of bronchial surface epithelia. Significant differences between stains were not observed suggesting common co-expression MUC5AC and MUC5B proteins in goblet cells of airway surface epithelia. Gallbladder and stomach tissues are reported to have differential mucin enrichment, so we tested these tissues in wildtype ferrets. Stomach tissues were enriched in MUC5AC and gallbladder tissues enriched in MUC5B, mucin enrichment similar to human tissues. Mucin immunostaining techniques were further qualified for specificity using lung tissue from recently generated MUC5AC
Identifiants
pubmed: 37349833
doi: 10.1186/s13104-023-06388-x
pii: 10.1186/s13104-023-06388-x
pmc: PMC10286488
doi:
Substances chimiques
MUC5B protein, human
0
Mucin-5B
0
MUC5AC protein, human
0
Mucin 5AC
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
111Subventions
Organisme : NIEHS NIH HHS
ID : P30 ES005605
Pays : United States
Organisme : NIH HHS
ID : DK054759
Pays : United States
Informations de copyright
© 2023. The Author(s).
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