Display of Oligo-α-1,6-Glycosidase from Exiguobacterium sibiricum on the Surface of Escherichia coli Cells.

Escherichia coli / metabolism Type V Secretion Systems / metabolism Glycoside Hydrolases / metabolism
autotransporter bacterial display oligo-α-1,6-glucosidase secretion

Journal

Biochemistry. Biokhimiia
ISSN: 1608-3040
Titre abrégé: Biochemistry (Mosc)
Pays: United States
ID NLM: 0376536

Informations de publication

Date de publication:
May 2023
Historique:
medline: 20 6 2023
pubmed: 19 6 2023
entrez: 18 6 2023
Statut: ppublish

Résumé

Cell-surface display using anchor motifs of outer membrane proteins allows exposure of target peptides and proteins on the surface of microbial cells. Previously, we obtained and characterized highly catalytically active recombinant oligo-α-1,6-glycosidase from the psychrotrophic bacterium Exiguobacterium sibiricum (EsOgl). It was also shown that the autotransporter AT877 from Psychrobacter cryohalolentis and its deletion variants efficiently displayed type III fibronectin (

Identifiants

DOI: 10.1134/S0006297923050140 PMID: 37331717
pubmed: 37331717
pii: BCM88050875
doi: 10.1134/S0006297923050140
doi:

Substances chimiques

Type V Secretion Systems 0
Glycoside Hydrolases EC 3.2.1.-
fasudil Q0CH43PGXS

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

716-722

Auteurs

Lyudmila N Shingarova (LN)

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia. lshingarova@gmail.com.

Lada E Petrovskaya (LE)

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.

Elena A Kryukova (EA)

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.

Sultan S Gapizov (SS)

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.
Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia.

Dmitry A Dolgikh (DA)

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.
Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia.

Mikhail P Kirpichnikov (MP)

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.
Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia.

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Classifications MeSH

Recherchemedicale.com › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Display of Oligo-α-1,6-Glycosidase from...
Recherchemedicale.com › Structures macromoléculaires › Complexes multiprotéiques › Systèmes de translocation des protéines › Systèmes bactériens de sécrétion › Systèmes de sécrétion de type V › Display of Oligo-α-1,6-Glycosidase from...
Recherchemedicale.com › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › Display of Oligo-α-1,6-Glycosidase from...