Proteomic analysis of circulating immune cells identifies cellular phenotypes associated with COVID-19 severity.

Humans COVID-19 SARS-CoV-2 Leukocytes, Mononuclear Proteomics Phenotype

CP: Immunology

Journal

Cell reports

ISSN: 2211-1247

Titre abrégé: Cell Rep

Pays: United States

ID NLM: 101573691

Informations de publication

Date de publication:
27 06 2023

Historique:

received: 15 11 2022

revised: 29 03 2023

accepted: 22 05 2023

medline: 4 10 2023

pubmed: 11 6 2023

entrez: 11 6 2023

Statut: ppublish

Résumé

Certain serum proteins, including C-reactive protein (CRP) and D-dimer, have prognostic value in patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Nonetheless, these factors are non-specific, providing limited mechanistic insight into the peripheral blood mononuclear cell (PBMC) populations that drive the pathogenesis of severe COVID-19. To identify cellular phenotypes associated with disease, we performed a comprehensive, unbiased analysis of total and plasma-membrane PBMC proteomes from 40 unvaccinated individuals with SARS-CoV-2, spanning the whole disease spectrum. Combined with RNA sequencing (RNA-seq) and flow cytometry from the same donors, we define a comprehensive multi-omic profile for each severity level, revealing that immune-cell dysregulation progresses with increasing disease. The cell-surface proteins CEACAMs1, 6, and 8, CD177, CD63, and CD89 are strongly associated with severe COVID-19, corresponding to the emergence of atypical CD3

Identifiants

DOI: 10.1016/j.celrep.2023.112613 PMID: 37302069 PMC: PMC10243220

pubmed: 37302069

pii: S2211-1247(23)00624-1

doi: 10.1016/j.celrep.2023.112613

pmc: PMC10243220

pii:

doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

Pagination

112613

Subventions

Organisme : Wellcome Trust

Pays : United Kingdom

Organisme : NHGRI NIH HHS

ID : U24 HG006673

Pays : United States