The free-living flagellate Paratrimastix pyriformis uses a distinct mitochondrial carrier to balance adenine nucleotide pools.
Mitochondrial carrier
Mitochondrion-related organelle
Nucleoside/nucleotide transport
Paratrimastix pyriformis
Journal
Archives of biochemistry and biophysics
ISSN: 1096-0384
Titre abrégé: Arch Biochem Biophys
Pays: United States
ID NLM: 0372430
Informations de publication
Date de publication:
01 07 2023
01 07 2023
Historique:
received:
07
04
2023
revised:
12
05
2023
accepted:
13
05
2023
medline:
1
6
2023
pubmed:
17
5
2023
entrez:
16
5
2023
Statut:
ppublish
Résumé
Paratrimastix pyriformis is a free-living flagellate thriving in low-oxygen freshwater sediments. It belongs to the group Metamonada along with human parasites, such as Giardia and Trichomonas. Like other metamonads, P. pyriformis has a mitochondrion-related organelle (MRO) which in this protist is primarily involved in one-carbon folate metabolism. The MRO contains four members of the solute carrier family 25 (SLC25) responsible for the exchange of metabolites across the mitochondrial inner membrane. Here, we characterise the function of the adenine nucleotide carrier PpMC1 by thermostability shift and transport assays. We show that it transports ATP, ADP and, to a lesser extent, AMP, but not phosphate. The carrier is distinct in function and origin from both ADP/ATP carriers and ATP-Mg/phosphate carriers, and it most likely represents a distinct class of adenine nucleotide carriers.
Identifiants
pubmed: 37192692
pii: S0003-9861(23)00137-6
doi: 10.1016/j.abb.2023.109638
pmc: PMC10251735
pii:
doi:
Substances chimiques
Adenosine Monophosphate
415SHH325A
Adenosine Triphosphate
8L70Q75FXE
Banques de données
figshare
['10.6084/m9.figshare.22317793']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
109638Subventions
Organisme : Medical Research Council
ID : MC_UU_00028/2UK
Pays : United Kingdom
Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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