Effect of cervical margin relocation on marginal adaptation and microleakage of indirect ceramic restorations.

This study evaluated the effect of cervical margin relocation with two different materials and contamination with hemostatic agent on the margin adaptation and microleakage of ceramic restorations. Mesial occlusal distal cavities were prepared in 60 human first molars and distributed to 3 groups (n = 20) according to the margin relocation procedure. The groups were: group F; flowable composite applied in two 2mm increments, group B; bulk fill flowable composite applied as a bulk increment of 4mm thickness and group C (control); no cervical margin relocation was done. Each group was subdivided into 2 subgroups (subgroup N; no hemostatic agent applied and subgroup H; hemostatic agent was applied). In all groups, ceramic inlays were prepared and cemented. The samples were subjected to thermocycling (10,000 cycles). The adaptation of the cervical margin was evaluated with scanning electron microscopy (200×). Samples were then assessed for microleakage analysis with dye penetration method. Marginal adaptation data were normally distributed and analyzed using two-way ANOVA followed by Tukey's post hoc test. Ordinal microleakage score data were analyzed using cumulative link models followed by analysis of deviance using Wald chi-square tests. Both cervical margin relocation and contamination with hemostatic agent had significant effects on margin adaptation of the cervical margin. Group C showed the highest adaptation with no significant difference from group F. The lowest adaptation was revealed in group B with a significant difference from group C. Subgroup N (in all groups) showed a statistically higher adaptation than subgroup H. Regarding microleakage assessment, cervical margin relocation had no significant effect but hemostatic agent application showed a significantly higher microleakage score for all groups. Both cervical margin relocation procedure and contamination with ALCL

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