A simplified protocol for the generation of cortical brain organoids.
cortical brain organoids
dorsal identity
feeder-independent
human brain development
induced pluripotent stem cells
self-patterning
Journal
Frontiers in cellular neuroscience
ISSN: 1662-5102
Titre abrégé: Front Cell Neurosci
Pays: Switzerland
ID NLM: 101477935
Informations de publication
Date de publication:
2023
2023
Historique:
received:
02
12
2022
accepted:
13
03
2023
medline:
21
4
2023
pubmed:
21
4
2023
entrez:
21
04
2023
Statut:
epublish
Résumé
Human brain organoid technology has the potential to generate unprecedented insight into normal and aberrant brain development. It opens up a developmental time window in which the effects of gene or environmental perturbations can be experimentally tested. However, detection sensitivity and correct interpretation of phenotypes are hampered by notable batch-to-batch variability and low reproducibility of cell and regional identities. Here, we describe a detailed, simplified protocol for the robust and reproducible generation of brain organoids with cortical identity from feeder-independent induced pluripotent stem cells (iPSCs). This self-patterning approach minimizes media supplements and handling steps, resulting in cortical brain organoids that can be maintained over prolonged periods and that contain radial glial and intermediate progenitors, deep and upper layer neurons, and astrocytes.
Identifiants
pubmed: 37082206
doi: 10.3389/fncel.2023.1114420
pmc: PMC10110973
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1114420Informations de copyright
Copyright © 2023 Eigenhuis, Somsen, van der Kroeg, Smeenk, Korporaal, Kushner, de Vrij and van den Berg.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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