The ancestral ESCRT protein TOM1L2 selects ubiquitinated cargoes for retrieval from cilia.
BBSome
ESCRT
GPCR
cilia
ubiquitin
Journal
Developmental cell
ISSN: 1878-1551
Titre abrégé: Dev Cell
Pays: United States
ID NLM: 101120028
Informations de publication
Date de publication:
24 04 2023
24 04 2023
Historique:
received:
13
10
2022
revised:
19
12
2022
accepted:
07
03
2023
pmc-release:
24
04
2024
medline:
27
4
2023
pubmed:
6
4
2023
entrez:
5
4
2023
Statut:
ppublish
Résumé
Many G protein-coupled receptors (GPCRs) reside within cilia of mammalian cells and must undergo regulated exit from cilia for the appropriate transduction of signals such as hedgehog morphogens. Lysine 63-linked ubiquitin (UbK63) chains mark GPCRs for regulated removal from cilia, but the molecular basis of UbK63 recognition inside cilia remains elusive. Here, we show that the BBSome-the trafficking complex in charge of retrieving GPCRs from cilia-engages the ancestral endosomal sorting factor target of Myb1-like 2 (TOM1L2) to recognize UbK63 chains within cilia of human and mouse cells. TOM1L2 directly binds to UbK63 chains and the BBSome, and targeted disruption of the TOM1L2/BBSome interaction results in the accumulation of TOM1L2, ubiquitin, and the GPCRs SSTR3, Smoothened, and GPR161 inside cilia. Furthermore, the single-cell alga Chlamydomonas also requires its TOM1L2 ortholog in order to clear ubiquitinated proteins from cilia. We conclude that TOM1L2 broadly enables the retrieval of UbK63-tagged proteins by the ciliary trafficking machinery.
Identifiants
pubmed: 37019113
pii: S1534-5807(23)00099-0
doi: 10.1016/j.devcel.2023.03.003
pmc: PMC10133032
mid: NIHMS1889413
pii:
doi:
Substances chimiques
Receptors, G-Protein-Coupled
0
Ubiquitin
0
Endosomal Sorting Complexes Required for Transport
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
677-693.e9Subventions
Organisme : NEI NIH HHS
ID : P30 EY002162
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY031462
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM067945
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM089933
Pays : United States
Informations de copyright
Copyright © 2023 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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