Diagnostic significance of hsa_circ_0000146 and hsa_circ_0000072 biomarkers for Diabetic Kidney Disease in patients with type 2 diabetes mellitus.

Diabetic Kidney Disease (DKD) is a significant challenge in healthcare. However, there are currently no reliable biomarkers for renal impairment diagnosis, prognosis, or staging in DKD patients. CircRNAs and microRNAs have emerged as noninvasive and efficient biomarkers. We explored Cannabinoid receptor 1 (CNR1), C reactive protein (CRP), hsa_circ_ 0000146 and 0000072, and hsa-miR-21 and 495 as diagnostic biomarkers in DKD. The serum concentrations of CRP and CNR1 were measured using ELISA. Rt-qPCR was used to evaluate the expression levels of CNR1, circRNAs, and miRNAs in 55 controls, 55 type 2 diabetes mellitus patients, and 55 DKD patients. Their diagnostic value was determined by their ROC curve. KEGG pathway was used to predict the functional mechanism of the circRNA's target genes. DKD patients exhibited a significant increase in CRP and CNR1 levels and the expression of miR-21 and 495. The expression levels of circ_0000146 and 0000072 decreased in DKD patients. ROC analysis revealed that circRNAs and miRNAs alone or CNR1 and CRP have significant diagnostic potential. The functional prediction results showed the involvement of hsa_circ_0000146 and 0000072 in various pathways that regulate DKD. Therefore, the examined circRNAs and miRNAs may represent a novel noninvasive biomarker for diagnosing and staging DKD. Dibetesna bolest bubrega (DKD) predstavlja značajan izazov u zdravstvu. Međutim, trenutno ne postoje pouzdani biomarkeri za dijagnozu, prognozu ili određivanje stadijuma oštećenja bubrega kod pacijenata sa DKD. CircRNA i mikroRNA su se pojavili kao neinvazivni i efikasni biomarkeri. Istražili smo kanabinoidni receptor 1 (CNR1), C reaktivni protein (CRP), hsa_circ_ 0000146 i 0000072, i hsa-miR-21 i 495 kao dijagnostičke biomarkere za DKD. Koncentracije CRP i CNR1 u serumu su merene korišćenjem ELISA. Rt-kPCR je korišćen za procenu nivoa ekspresije CNR1, circRNA i miRNA kod 55 kontrolnih pacijenata, 55 pacijenata sa dijabetes melitusom tipa 2 i 55 pacijenata sa DKD. Njihova dijagnostička vrednost određena je njihovom ROC krivom. Za predviđanje funkcionalnog mehanizma ciljnih gena circRNA korišćena je KEGG mapa puteva. Pacijenti sa DKD su pokazali značajno povećanje nivoa CRP i CNR1 i ekspresije miR-21 i 495. Nivoi ekspresije circ_0000146 i 0000072 su se smanjili kod pacijenata sa DKD. ROC analiza je otkrila da samo circRNA i miRNA ili CNR1 i CRP imaju značajan dijagnostički potencijal. Rezultati funkcionalnog predviđanja pokazali su učešće hsa_circ_0000146 i 0000072 u različitim putevima koji regulišu DKD. Stoga, ispitivani circRNA i miRNA mogu predstavljati novi neinvazivni biomarker za dijagnostikovanje i određivanje stadijuma DKD.

2023 Amul M. Badr, Omayma Elkholy, Mona Said, Sally A. Fahim, Mohamed El-Khatib, Dina Sabry, Radwa M. Gaber, published by CEON/CEES.

All the authors declare that they have no conflict of interest in this work.Conflict of Interest: The authors stated that they have no conflicts of interest regarding the publication of this article.