Development and Validation of a Gas Chromatography-Mass Spectrometry Method for Determining Acaricides in Bee Pollen.


Journal

Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009

Informations de publication

Date de publication:
09 Mar 2023
Historique:
received: 21 02 2023
revised: 07 03 2023
accepted: 07 03 2023
medline: 30 3 2023
entrez: 29 3 2023
pubmed: 30 3 2023
Statut: epublish

Résumé

Pesticides can be found in beehives for several reasons, including contamination from surrounding crops or for their use by beekeepers, which poses a risk to bee ecosystems and consumers. Therefore, efficient and sensitive methods are needed for determining pesticide residues in bee products. In this study, a new analytical method has been developed and validated to determine seven acaricides (atrazine, chlorpyrifos, chlorfenvinphos, α-endosulfan, bromopropylate, coumaphos, and τ-fluvalinate) in bee pollen using gas chromatography coupled to mass spectrometry. After an optimization study, the best sample treatment was obtained when using a modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method employing an ethyl acetate and cyclohexane as the extractant mixture, and a mixture of salts for the clean-up step. A chromatographic analysis (<21 min) was performed in an Agilent DB-5MS column, and it was operated under programmed temperature conditions. The method was fully validated in terms of selectivity, limits of detection (0.2-3.1 µg kg

Identifiants

pubmed: 36985469
pii: molecules28062497
doi: 10.3390/molecules28062497
pmc: PMC10056623
pii:
doi:

Substances chimiques

fluvalinate 364G5G03VC
Acaricides 0
Coumaphos L08SZ5Z5JC
Chlorfenvinphos F2G9XS1W91
Endosulfan OKA6A6ZD4K
Pesticides 0
Pesticide Residues 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
ID : RTA2017-00004-C02-02

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Auteurs

Adrián Fuente-Ballesteros (A)

Analytical Chemistry Group (TESEA), I.U. CINQUIMA, Faculty of Sciences, University of Valladolid, 47011 Valladolid, Spain.

Camille Augé (C)

SIGMA Clermont, Clermont-Ferrand Campus, 63178 Aubiere, France.

José Bernal (J)

Analytical Chemistry Group (TESEA), I.U. CINQUIMA, Faculty of Sciences, University of Valladolid, 47011 Valladolid, Spain.

Ana M Ares (AM)

Analytical Chemistry Group (TESEA), I.U. CINQUIMA, Faculty of Sciences, University of Valladolid, 47011 Valladolid, Spain.

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Classifications MeSH