Disclosing quantitative RT-PCR raw data during manuscript submission: a call for action.

Humans Reverse Transcriptase Polymerase Chain Reaction Reproducibility of Results RNA Real-Time Polymerase Chain Reaction / methods
RNA RT-qPCR accuracy quantification

Journal

Molecular oncology
ISSN: 1878-0261
Titre abrégé: Mol Oncol
Pays: United States
ID NLM: 101308230

Informations de publication

Date de publication:
05 2023
Historique:
received: 17 02 2023
accepted: 09 03 2023
medline: 8 5 2023
pubmed: 15 3 2023
entrez: 14 3 2023
Statut: ppublish

Résumé

Accuracy and transparency of scientific data are becoming more and more relevant with the increasing concern regarding the evaluation of data reproducibility in many research areas. This concern is also true for quantifying coding and noncoding RNAs, with the remarkable increase in publications reporting RNA profiling and sequencing studies. To address the problem, we propose the following recommendations: (a) accurate documentation of experimental procedures in Materials and methods (and not only in the supplementary information, as many journals have a strict mandate for making Materials and methods as visible as possible in the main text); (b) submission of RT-qPCR raw data for all experiments reported; and (c) adoption of a unified, simple format for submitted RT-qPCR raw data. The Real-time PCR Data Essential Spreadsheet Format (RDES) was created for this purpose.

Identifiants

DOI: 10.1002/1878-0261.13418 PMID: 36916500 PMC: PMC10158759
pubmed: 36916500
doi: 10.1002/1878-0261.13418
pmc: PMC10158759
doi:

Substances chimiques

RNA 63231-63-0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

713-717

Informations de copyright

© 2023 The Authors. Molecular Oncology published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.

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Auteurs

Andreas Untergasser (A)

Zentrum für Molekulare Biologie der Universität Heidelberg, Germany.
Genomics Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.

Jan Hellemans (J)

CellCarta, Zwijnaarde, Belgium.

Michael W Pfaffl (MW)

Division of Animal Physiology and Immunology, School of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany.

Jan M Ruijter (JM)

Department of Medical Biology, Amsterdam UMC, location AMC, University of Amsterdam, The Netherlands.

Maurice J B van den Hoff (MJB)

Department of Medical Biology, Amsterdam UMC, location AMC, University of Amsterdam, The Netherlands.

Mihnea P Dragomir (MP)

Institute of Pathology, Berlin Institute of Health, Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Germany.
Berlin Institute of Health, Germany.
German Cancer Research Center (DKFZ), German Cancer Consortium (DKTK), Heidelberg, Germany.

Douglas Adamoski (D)

Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, Brazil.

Sandra Martha Gomes Dias (SMG)

Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, Brazil.

Rui Manuel Reis (RM)

Molecular Oncology Research Center, Barretos Cancer Hospital, Brazil.
Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal.
3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal.
ORCID: 0000-0002-9639-7940

Manuela Ferracin (M)

Department of Medical and Surgical Sciences (DIMEC), University of Bologna, Italy.
IRCCS Azienda Ospedaliero-Universitaria di Bologna, Italy.
ORCID: 0000-0002-1595-6887

Emmanuel Dias-Neto (E)

Laboratory of Medical Genomics, Centro Internacional de Pesquisa, AC Camargo Cancer Center, São Paulo, Brazil.
Laboratory of Neurosciences (LIM27), Institute of Psychiatry, Faculdade de Medicina, Universidade de São Paulo, Brazil.

Ian Marsh (I)

New South Wales Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Narellan, Australia.

Mikael Kubista (M)

TATAA Biocenter AB, Göteborg, Sweden.
Institute of Biotechnology CAS, v. v. i., Vestec, Czech Republic.

Muller Fabbri (M)

Center for Cancer and Immunology Research, Children's National Hospital, Washington, DC, USA.

Ajay Goel (A)

Department of Molecular Diagnostics and Experimental Therapeutics, Beckman Research Institute of City of Hope, Monrovia, CA, USA.
City of Hope Comprehensive Cancer Center, Duarte, CA, USA.
ORCID: 0000-0003-1396-6341

Ondřej Slabý (O)

Department of Biology, Faculty of Medicine, Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno, Czech Republic.

Erik Knutsen (E)

Department of Medical Biology, Faculty of Health Sciences, UiT-The Arctic University of Norway, Tromsø, Norway.
ORCID: 0000-0002-4903-0150

Baoqing Chen (B)

Department of Radiation Oncology, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.

Massimo Negrini (M)

Laboratory for Technologies of Advanced Therapies (LTTA), Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Italy.

Koshi Mimori (K)

Department of Surgery, Kyushu University Beppu Hospital, Japan.

Martin Pichler (M)

Division of Oncology, Department of Internal Medicine, Medical University of Graz, Austria.
Translational Oncology, University Hospital of Augsburg Stenglinstrasse, Germany.

Maria Papatriantafyllou (M)

Editorial Manager, Molecular Oncology, Cambridge, UK.

Simone Anfossi (S)

Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.

Thomas D Schmittgen (TD)

Department of Pharmaceutics, University of Florida College of Pharmacy, Gainesville, FL, USA.

Jim Huggett (J)

National Measurement Laboratory, LGC, Teddington, UK.
School of Biosciences and Medicine, Faculty of Health and Medical Sciences, University of Surrey, Guildford, UK.

Stephen Bustin (S)

Medical Technology Research Centre, Anglia Ruskin University, Chelmsford, Essex, UK.

Jo Vandesompele (J)

CellCarta, Zwijnaarde, Belgium.
Department of Biomolecular Medicine, Ghent University, Belgium.
OncoRNALab, Cancer Research Institute Ghent (CRIG), Belgium.

George A Calin (GA)

Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
The Center for Non-codingRNAs, University of Texas MD Anderson Cancer Center, Houston, TX, USA.
ORCID: 0000-0002-7427-0578

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Recherchemedicale.com › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › RT-PCR › Disclosing quantitative RT-PCR raw data during...
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