Development of a Versatile Protein Labeling Tool for Live-Cell Imaging Using Fluorescent β-Lactamase Inhibitors.
Autophagy
Fluorescence Imaging Probe
Lysosomal Trafficking
Protein Labeling
β-Lactamase Inhibitor
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
24 04 2023
24 04 2023
Historique:
received:
03
02
2023
medline:
18
4
2023
pubmed:
8
3
2023
entrez:
7
3
2023
Statut:
ppublish
Résumé
To understand the function of protein in live cells, real-time monitoring of protein dynamics and sensing of their surrounding environment are important methods. Fluorescent labeling tools are thus needed that possess fast labeling kinetics, high efficiency, and long-term stability. We developed a versatile chemical protein-labeling tool based on fluorophore-conjugated diazabicyclooctane β-lactamase inhibitors (BLIs) and wild-type TEM-1 β-lactamase protein tag. The fluorescent probes efficiently formed a stable carbamoylated complex with β-lactamase, and the labeled proteins were visualized over a long period of time in live cells. Moreover, use of an α-fluorinated carboxylate ester-based BLI prodrug enabled the probe to permeate cell membranes and stably label intracellular proteins after unexpected spontaneous ester hydrolysis. Lastly, combining the labeling tool with a pH-activatable fluorescent probe allowed visual monitoring of lysosomal protein translocation during autophagy.
Identifiants
pubmed: 36880808
doi: 10.1002/anie.202301704
doi:
Substances chimiques
beta-Lactamase Inhibitors
0
Proteins
0
Fluorescent Dyes
0
Penicillins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202301704Informations de copyright
© 2023 Wiley-VCH GmbH.
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