Copper toxicity leads to accumulation of free amino acids and polyphenols in Phaeodactylum tricornutum diatoms.
Amino acid
Antioxidant activity
Copper toxicity
Microalgae
Phaeodactylum tricornutum
Polyphenol
Journal
Environmental science and pollution research international
ISSN: 1614-7499
Titre abrégé: Environ Sci Pollut Res Int
Pays: Germany
ID NLM: 9441769
Informations de publication
Date de publication:
Apr 2023
Apr 2023
Historique:
received:
27
09
2022
accepted:
10
02
2023
medline:
18
4
2023
pubmed:
23
2
2023
entrez:
22
2
2023
Statut:
ppublish
Résumé
This work is focused on the effect of lethal and sub-lethal copper (Cu) concentrations on the free amino acid and polyphenol production by the marine diatom Phaeodactylum tricornutum (P. tricornutum) after 12, 18, and 21 days of exposure. The concentrations of 10 amino acids (arginine, aspartic acid, glutamic acid, histidine, lysine, methionine, proline, valine, isoleucine, and phenylalanine) and 10 polyphenols (gallic acid, protocatechuic acid, p-coumaric acid, ferulic acid, catechin, vanillic acid, epicatechin syringic acid, rutin, and gentisic acid) were measured by RP-HPLC. Under lethal doses of Cu, free amino acids reached levels significantly higher than those in the control cells (up to 21.9 times higher), where histidine and methionine showed the highest increases (up to 37.4 and 65.8 times higher, respectively). The total phenolic content also increased up to 11.3 and 5.59 times higher compared to the reference cells, showing gallic acid the highest increase (45.8 times greater). The antioxidant activities of cells exposed to Cu were also enhanced with increasing doses of Cu(II). They were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging ability (RSA), cupric ion reducing antioxidant capacity (CUPRAC), and ferric reducing antioxidant power (FRAP) assays. Malonaldehyde (MDA) exhibited the same tendency: cells grown at the highest lethal Cu concentration yielded the highest MDA level. These findings reflect the involvement of amino acids and polyphenols in protective mechanisms to overcome the toxicity of copper in marine microalgae.
Identifiants
pubmed: 36809613
doi: 10.1007/s11356-023-25939-0
pii: 10.1007/s11356-023-25939-0
pmc: PMC10104907
doi:
Substances chimiques
Polyphenols
0
Antioxidants
0
Copper
789U1901C5
Amino Acids
0
Histidine
4QD397987E
Gallic Acid
632XD903SP
Methionine
AE28F7PNPL
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
51261-51270Subventions
Organisme : Ministerio de Economía, Industria y Competitividad
ID : CTM2017-83476-P
Organisme : Universidad de Las Palmas de Gran Canaria
ID : PIFULPGC-2019
Informations de copyright
© 2023. The Author(s).
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