Developmental pruning of sensory neurites by mechanical tearing in Drosophila.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
06 03 2023
06 03 2023
Historique:
received:
03
05
2022
revised:
24
08
2022
accepted:
04
01
2023
entrez:
17
1
2023
pubmed:
18
1
2023
medline:
20
1
2023
Statut:
ppublish
Résumé
Mechanical forces actively shape cells during development, but little is known about their roles during neuronal morphogenesis. Developmental neurite pruning, a critical circuit specification mechanism, often involves neurite abscission at predetermined sites by unknown mechanisms. Pruning of Drosophila sensory neuron dendrites during metamorphosis is triggered by the hormone ecdysone, which induces local disassembly of the dendritic cytoskeleton. Subsequently, dendrites are severed at positions close to the soma by an unknown mechanism. We found that ecdysone signaling causes the dendrites to become mechanically fragile. Severing occurs during periods of increased pupal morphogenetic tissue movements, which exert mechanical forces on the destabilized dendrites. Tissue movements and dendrite severing peak during pupal ecdysis, a period of strong abdominal contractions, and abolishing ecdysis causes non-cell autonomous dendrite pruning defects. Thus, our data establish mechanical tearing as a novel mechanism during neurite pruning.
Identifiants
pubmed: 36648440
pii: 213805
doi: 10.1083/jcb.202205004
pmc: PMC9856751
pii:
doi:
Substances chimiques
Ecdysone
3604-87-3
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2023 Krämer et al.
Références
Cell Rep. 2018 Aug 28;24(9):2287-2299.e4
pubmed: 30157424
EMBO J. 2017 Jul 3;36(13):1981-1991
pubmed: 28554895
Neuron. 2006 Jun 15;50(6):883-95
pubmed: 16772170
Annu Rev Cell Dev Biol. 2015;31:779-805
pubmed: 26436703
Proc Natl Acad Sci U S A. 2011 Jun 7;108(23):9673-8
pubmed: 21606367
Curr Opin Neurobiol. 2014 Aug;27:192-8
pubmed: 24793180
Neuron. 2003 May 22;38(4):567-80
pubmed: 12765609
Cell. 2002 Jun 28;109(7):887-900
pubmed: 12110185
Development. 2018 May 11;145(9):
pubmed: 29712642
Trends Cell Biol. 2019 Apr;29(4):291-297
pubmed: 30683460
Development. 2005 Aug;132(16):3631-42
pubmed: 16033801
Curr Biol. 2019 Mar 18;29(6):935-944.e4
pubmed: 30853438
Genomics Proteomics Bioinformatics. 2016 Feb;14(1):55-61
pubmed: 26829645
J Cell Sci. 1979 Jun;37:391-410
pubmed: 479327
J Vis Exp. 2015 Dec 17;(106):e53749
pubmed: 26709554
Nature. 2007 Jul 12;448(7150):151-6
pubmed: 17625558
Cell Rep. 2022 May 31;39(9):110887
pubmed: 35649352
Elife. 2019 Mar 28;8:
pubmed: 30920370
Elife. 2021 Jul 08;10:
pubmed: 34236312
Neuron. 2014 Feb 5;81(3):544-560
pubmed: 24412417
Development. 1995 May;121(5):1455-65
pubmed: 7789275
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
EMBO Rep. 2021 Oct 5;22(10):e52679
pubmed: 34338441
Cell. 2016 Apr 21;165(3):754-754.e1
pubmed: 27104981
Nature. 2019 Apr;568(7752):395-399
pubmed: 30918398
Proc Natl Acad Sci U S A. 2005 Oct 18;102(42):15230-5
pubmed: 16210248
Development. 2003 Jun;130(12):2645-56
pubmed: 12736209
Biophys J. 2012 Sep 5;103(5):868-77
pubmed: 23009836
Nat Methods. 2019 Jul;16(7):565-566
pubmed: 31217592
Annu Rev Cell Dev Biol. 2020 Oct 6;36:61-83
pubmed: 32603614
Nat Neurosci. 2009 Dec;12(12):1497-505
pubmed: 19881505
Cell. 2016 May 5;165(4):1028-1028.e1
pubmed: 27153501
EMBO Rep. 2020 May 6;21(5):e48870
pubmed: 32207238
Proc Natl Acad Sci U S A. 2022 Oct 25;119(43):e2210421119
pubmed: 36252008
Cell Rep. 2021 Nov 16;37(7):110024
pubmed: 34788610
Neuron. 2012 Jan 12;73(1):64-78
pubmed: 22243747
Nat Commun. 2015 Mar 12;6:6515
pubmed: 25761586
Mech Dev. 2007 May;124(5):390-406
pubmed: 17350233
Proc Natl Acad Sci U S A. 2009 Apr 14;106(15):6363-8
pubmed: 19329489
Cell. 2003 Mar 21;112(6):805-18
pubmed: 12654247
Nat Neurosci. 2006 Oct;9(10):1234-6
pubmed: 16980964
Proc Natl Acad Sci U S A. 2014 May 20;111(20):7331-6
pubmed: 24799714
Dev Dyn. 2010 Oct;239(10):2685-94
pubmed: 20803583
Curr Biol. 2019 Mar 18;29(6):945-956.e3
pubmed: 30853433
Curr Biol. 2003 Apr 15;13(8):618-26
pubmed: 12699617
Development. 2007 Jan;134(1):55-64
pubmed: 17164414
Neuron. 2006 Jun 15;50(6):855-67
pubmed: 16772168
Neuron. 2009 Feb 26;61(4):519-26
pubmed: 19249273
PLoS Biol. 2004 Aug;2(8):E239
pubmed: 15269788
Neuron. 2012 Jan 12;73(1):79-91
pubmed: 22243748