Electrical signals in the ER are cell type and stimulus specific with extreme spatial compartmentalization in neurons.
BK channels
CP: Cell biology
CP: Neuroscience
ER
HEK cells
dendrites
electrical compartmentalization
genetically encoded voltage sensor
membrane potential
neurons
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
31 01 2023
31 01 2023
Historique:
received:
12
01
2022
revised:
04
10
2022
accepted:
15
12
2022
pubmed:
15
1
2023
medline:
7
2
2023
entrez:
14
1
2023
Statut:
ppublish
Résumé
The endoplasmic reticulum (ER) is a tortuous organelle that spans throughout a cell with a continuous membrane containing ion channels, pumps, and transporters. It is unclear if stimuli that gate ER ion channels trigger substantial membrane potential fluctuations and if those fluctuations spread beyond their site of origin. Here, we visualize ER membrane potential dynamics in HEK cells and cultured rat hippocampal neurons by targeting a genetically encoded voltage indicator specifically to the ER membrane. We report the existence of clear cell-type- and stimulus-specific ER membrane potential fluctuations. In neurons, direct stimulation of ER ryanodine receptors generates depolarizations that scale linearly with stimulus strength and reach tens of millivolts. However, ER potentials do not spread beyond the site of receptor activation, exhibiting steep attenuation that is exacerbated by intracellular large conductance K
Identifiants
pubmed: 36640310
pii: S2211-1247(22)01844-7
doi: 10.1016/j.celrep.2022.111943
pmc: PMC10033362
mid: NIHMS1870510
pii:
doi:
Substances chimiques
Calcium
SY7Q814VUP
Ryanodine Receptor Calcium Release Channel
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
111943Subventions
Organisme : NINDS NIH HHS
ID : DP2 NS097029
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM133351
Pays : United States
Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors have no competing interests to declare.
Références
J Neurosci. 1997 Nov 15;17(22):8729-38
pubmed: 9348342
Proc Natl Acad Sci U S A. 2009 Sep 1;106(35):15055-60
pubmed: 19706463
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Circ Res. 1992 Sep;71(3):585-9
pubmed: 1323433
Elife. 2017 Jul 27;6:
pubmed: 28749338
Biochem J. 1998 Mar 1;330 ( Pt 2):1015-21
pubmed: 9480924
J Biol Chem. 1999 May 21;274(21):15080-4
pubmed: 10329713
Nat Neurosci. 2014 Aug;17(8):1055-63
pubmed: 24952642
Cell. 2019 Dec 12;179(7):1590-1608.e23
pubmed: 31835034
Trends Plant Sci. 2014 Aug;19(8):508-15
pubmed: 24794130
Elife. 2019 Oct 30;8:
pubmed: 31663850
J Neurosci. 1997 Jan 1;17(1):190-203
pubmed: 8987748
Neuron. 2018 Jun 27;98(6):1080-1098
pubmed: 29953871
Nat Nanotechnol. 2021 Jan;16(1):96-103
pubmed: 33139937
Nat Commun. 2017 Nov 15;8(1):1505
pubmed: 29138405
J Neurosci. 1992 Feb;12(2):489-505
pubmed: 1311032
Eur J Cell Biol. 2001 Feb;80(2):151-5
pubmed: 11302519
Science. 2022 Jan 07;375(6576):82-86
pubmed: 34762487
Proc Natl Acad Sci U S A. 2017 Jun 13;114(24):E4859-E4867
pubmed: 28559323
J Cell Biol. 1991 Jun;113(5):1145-57
pubmed: 1645737
Biomed Biochim Acta. 1989;48(5-6):S313-8
pubmed: 2757605
J Am Chem Soc. 2022 Jul 13;144(27):12138-12146
pubmed: 35776693
Nat Commun. 2019 Jul 19;10(1):3223
pubmed: 31324793
Neuron. 2006 Apr 20;50(2):291-307
pubmed: 16630839
Cell. 2012 Jan 20;148(1-2):309-21
pubmed: 22265418
Sci Rep. 2018 May 2;8(1):6911
pubmed: 29720664
J Physiol. 2009 Apr 1;587(Pt 7):1439-59
pubmed: 19204047
J Neurosci. 2015 Feb 18;35(7):3001-9
pubmed: 25698737
Biochem J. 2008 Sep 15;414(3):441-52
pubmed: 18518861
Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7510-4
pubmed: 7519781
J Pharmacol Exp Ther. 2005 Feb;312(2):502-8
pubmed: 15466247
Nature. 1987 Feb 5-11;325(6104):531-3
pubmed: 2880300
Biochem Pharmacol. 2010 Mar 15;79(6):908-20
pubmed: 19883631
Biomed Eng Online. 2003 May 17;2:13
pubmed: 12801419
J Biol Chem. 1990 Jul 5;265(19):11083-90
pubmed: 1694175
Nat Methods. 2016 Dec;13(12):989-992
pubmed: 27798609
J Math Neurosci. 2020 Jan 28;10(1):1
pubmed: 31993756
Biochem J. 2014 Nov 15;464(1):13-22
pubmed: 25164254
Proc Natl Acad Sci U S A. 2019 Mar 5;116(10):4238-4243
pubmed: 30770441
Cell Calcium. 2012 Jun;51(6):427-33
pubmed: 22387011
Pharmacol Rep. 2021 Jun;73(3):881-890
pubmed: 33713314
J Electron Microsc (Tokyo). 1976;25(3):141-9
pubmed: 1025229
Biophys J. 1974 Oct;14(10):759-90
pubmed: 4424185
Mol Biol Cell. 1995 Oct;6(10):1315-32
pubmed: 8573789
Nature. 2013 Jul 18;499(7458):295-300
pubmed: 23868258
Commun Biol. 2021 Mar 23;4(1):389
pubmed: 33758369
PLoS Comput Biol. 2008 Mar 28;4(3):e1000036
pubmed: 18369427
Annu Rev Cell Dev Biol. 1996;12:27-54
pubmed: 8970721
Biophys J. 1998 May;74(5):2335-51
pubmed: 9591661
Nature. 2007 Jul 5;448(7149):78-82
pubmed: 17611541
J Biol Chem. 1997 Sep 19;272(38):23880-6
pubmed: 9295337
Biochemistry. 1994 May 17;33(19):5819-28
pubmed: 7514038
PLoS Genet. 2018 Aug 29;14(8):e1007504
pubmed: 30157172
J Membr Biol. 1978 Apr 20;40(1):1-23
pubmed: 650672
J Membr Biol. 1987;99(2):103-11
pubmed: 2448472
Cell Mol Life Sci. 2012 Apr;69(8):1353-75
pubmed: 22105709
Curr Biol. 2005 Jul 12;15(13):1235-41
pubmed: 16005298
J Biol Chem. 1998 Jan 9;273(2):950-6
pubmed: 9422755
J Neurosci Methods. 2006 Jun 30;154(1-2):1-18
pubmed: 16554092
J Cell Biol. 1981 Sep;90(3):577-94
pubmed: 6974735
Biophys J. 1999 May;76(5):2843-51
pubmed: 10233100
J Cell Sci. 2012 Feb 1;125(Pt 3):625-33
pubmed: 22331352
Cell. 2018 Jul 12;174(2):465-480.e22
pubmed: 30007418
J Histochem Cytochem. 1983 Sep;31(9):1077-88
pubmed: 6309951
Nat Rev Neurosci. 2012 Feb 08;13(3):157-68
pubmed: 22314443
Nat Methods. 2012 Oct;9(10):1005-12
pubmed: 22961245
Nature. 2005 Oct 6;437(7060):902-5
pubmed: 16208375
J Gen Physiol. 2018 Aug 6;150(8):1163-1177
pubmed: 29899059
Biophys J. 2008 Oct;95(8):3706-14
pubmed: 18621826
PLoS One. 2012;7(3):e33429
pubmed: 22438928
J Cell Biol. 1994 Nov;127(3):653-65
pubmed: 7962050
Arch Biochem Biophys. 2008 Mar 1;471(1):50-6
pubmed: 18187033
J Biol Chem. 2012 Oct 26;287(44):37233-44
pubmed: 22948152
EMBO J. 1994 Apr 1;13(7):1696-705
pubmed: 8157008
Exp Neurol. 2008 Aug;212(2):543-7
pubmed: 18572168
J Neurosci. 1993 Nov;13(11):4636-46
pubmed: 8229189
Nature. 2006 Sep 14;443(7108):230-3
pubmed: 16921383