Decellularization of Human Pancreatic Fragments with Pronounced Signs of Structural Changes.
decellularization
extracellular matrix
human adipose derived stem cell
pancreas
pancreatic islets
tissue-specific scaffold
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
21 Dec 2022
21 Dec 2022
Historique:
received:
17
11
2022
revised:
12
12
2022
accepted:
16
12
2022
entrez:
8
1
2023
pubmed:
9
1
2023
medline:
11
1
2023
Statut:
epublish
Résumé
A significant lack of donor organs restricts the opportunity to obtain tissue-specific scaffolds for tissue-engineering technologies. One of the acceptable solutions is the development of decellularization protocols for a human donor pancreas unsuitable for transplantation. A protocol of obtaining a biocompatible tissue-specific scaffold from decellularized fragments with pronounced human pancreas lipomatosis signs with preserved basic fibrillary proteins of a pancreatic tissue extracellular matrix was developed. The scaffold supports the adhesion and proliferation of human adipose derived stem cell (hADSCs) and prolongs the viability and insulin-producing function of pancreatic islets. Experiments conducted allow for the reliance on the prospects of using the donor pancreas unsuitable for transplantation in the technologies of tissue engineering and regenerative medicine, including the development of a tissue equivalent of a pancreas.
Identifiants
pubmed: 36613557
pii: ijms24010119
doi: 10.3390/ijms24010119
pmc: PMC9820198
pii:
doi:
Substances chimiques
Pancreatic Hormones
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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