Highly sensitive and quick in ovo sexing of domestic chicken eggs by two-wavelength fluorescence spectroscopy.
Fluorescence spectroscopy
Heme synthesis
In ovo sexing
Protoporphyrin IX
Two-wavelength spectroscopy
Journal
Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327
Informations de publication
Date de publication:
Feb 2023
Feb 2023
Historique:
received:
14
10
2022
accepted:
16
11
2022
revised:
08
11
2022
pubmed:
4
12
2022
medline:
18
1
2023
entrez:
3
12
2022
Statut:
ppublish
Résumé
The in ovo sexing of chicken eggs is a current task and a prerequisite to overcome the mass killing of male day-old chicks from laying lines. Although various methods have been developed and tested in recent years, practicable methods for sex determination are still missing which can be applicated in poultry hatcheries before the chicken embryo is capable of nociception and pain sensation. Optical spectroscopic methods enable an early determination of the sex. In this study, a novel method based on two-wavelength in ovo fluorescence excitation is described. More than 1600 eggs were examined. In ovo fluorescence was sequentially excited at 532 nm and 785 nm. The fluorescence intensities of the spectral regions behave inversely with respect to sex. It is shown that the observed sex-related differences in the fluorescence intensities are based on the embryonic hemoglobin synthesis. The accuracy of sex determination is 96% for both sexes. The hatching rate is not reduced compared to an equivalent reference group.
Identifiants
pubmed: 36462048
doi: 10.1007/s00216-022-04446-0
pii: 10.1007/s00216-022-04446-0
pmc: PMC9839792
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
603-613Informations de copyright
© 2022. The Author(s).
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