Single-cell RNA sequencing identifies a paracrine interaction that may drive oncogenic notch signaling in human adenoid cystic carcinoma.

Humans Carcinoma, Adenoid Cystic / genetics Oncogenes Carcinogenesis Exome Sequencing Sequence Analysis, RNA
CP: Cancer MYB Notch1 adenoid cystic carcinoma head and neck luminal myoepithelial paracrine interaction single cell sequencing

Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
29 11 2022
Historique:
received: 30 12 2021
revised: 11 08 2022
accepted: 07 11 2022
entrez: 30 11 2022
pubmed: 1 12 2022
medline: 3 12 2022
Statut: ppublish

Résumé

Salivary adenoid cystic carcinoma (ACC) is a rare, biologically unique biphasic tumor that consists of malignant myoepithelial and luminal cells. MYB and Notch signaling have been implicated in ACC pathophysiology, but in vivo descriptions of these two programs in human tumors and investigation into their active coordination remain incomplete. We utilize single-cell RNA sequencing to profile human head and neck ACC, including a comparison of primary ACC with a matched local recurrence. We define expression heterogeneity in these rare tumors, uncovering diversity in myoepithelial and luminal cell expression. We find differential expression of Notch ligands DLL1, JAG1, and JAG2 in myoepithelial cells, suggesting a paracrine interaction that may support oncogenic Notch signaling. We validate this selective expression in three published cohorts of patients with ACC. Our data provide a potential explanation for the biphasic nature of low- and intermediate-grade ACC and may help direct new therapeutic strategies against these tumors.

Identifiants

DOI: 10.1016/j.celrep.2022.111743 PMID: 36450256 PMC: PMC9760094
pubmed: 36450256
pii: S2211-1247(22)01621-7
doi: 10.1016/j.celrep.2022.111743
pmc: PMC9760094
mid: NIHMS1854087
pii:
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

111743

Subventions

Organisme : NCI NIH HHS
ID : K08 CA237732
Pays : United States
Organisme : NCI NIH HHS
ID : L30 CA209706
Pays : United States
Organisme : NIDCR NIH HHS
ID : R21 DE031072
Pays : United States

Informations de copyright

Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests J.C.A. is a consultant to Ayala Pharmaceuticals, Remix Therapeutics, and Cellestia, Inc. B.E.B. declares outside interests in Fulcrum Therapeutics, Arsenal Biosciences, HiFiBio, Cell Signaling Technologies, and Chroma Medicine.

Références

Mol Cell Biol. 2016 May 02;36(10):1509-25
pubmed: 26976638
Cancer Discov. 2016 Feb;6(2):176-87
pubmed: 26631070
Front Immunol. 2021 Sep 06;12:618367
pubmed: 34552580
Skelet Muscle. 2016 Nov 8;6:35
pubmed: 27826411
Clin Cancer Res. 2021 May 1;27(9):2636-2647
pubmed: 33622705
Nat Methods. 2013 Nov;10(11):1096-8
pubmed: 24056875
Cancer Biol Ther. 2008 Aug;7(8):1212-25
pubmed: 18458534
J Genet Genomics. 2020 Jul 20;47(7):397-401
pubmed: 33077413
Mol Cell. 2010 May 28;38(4):576-89
pubmed: 20513432
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Head Neck. 2016 Apr;38(4):620-7
pubmed: 25487882
PLoS Biol. 2004 Feb;2(2):E7
pubmed: 14737219
Nat Protoc. 2014 Jan;9(1):171-81
pubmed: 24385147
Cell Death Dis. 2021 Apr 6;12(4):352
pubmed: 33824285
J Clin Invest. 2019 Oct 1;129(10):4276-4289
pubmed: 31483290
Bioinformatics. 2014 Apr 1;30(7):923-30
pubmed: 24227677
Anticancer Res. 2016 Jun;36(6):2793-8
pubmed: 27272790
Mol Cell Oncol. 2018 Mar 7;5(3):e1448244
pubmed: 30250901
Head Neck Pathol. 2008 Mar;2(1):13-8
pubmed: 20614336
BMC Cancer. 2021 Apr 24;21(1):458
pubmed: 33894748
PLoS Comput Biol. 2021 Jul 20;17(7):e1009228
pubmed: 34283835
Am J Surg Pathol. 2017 Nov;41(11):1473-1482
pubmed: 28914715
Oncogene. 2006 Apr 6;25(15):2273-84
pubmed: 16288205
Front Oncol. 2022 Apr 14;12:862751
pubmed: 35494000
Annu Rev Pathol. 2017 Jan 24;12:245-275
pubmed: 27959635
Ann Transl Med. 2021 May;9(10):889
pubmed: 34164523
Science. 2014 Jun 20;344(6190):1396-401
pubmed: 24925914
Oncotarget. 2017 Sep 14;8(47):81725-81726
pubmed: 29137213
Nat Biotechnol. 2018 Jun;36(5):411-420
pubmed: 29608179
Proc Natl Acad Sci U S A. 2009 Nov 3;106(44):18740-4
pubmed: 19841262
Nat Commun. 2017 May 05;8:15081
pubmed: 28474673
Nat Genet. 2021 Dec;53(12):1698-1711
pubmed: 34857954
Nat Genet. 2013 Jul;45(7):791-8
pubmed: 23685749
Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50
pubmed: 16199517
Oral Oncol. 2020 Jul;106:104690
pubmed: 32283496
Nat Genet. 2017 May;49(5):708-718
pubmed: 28319088
Histopathology. 2012 Mar;60(4):609-16
pubmed: 22260414
Oncotarget. 2016 Oct 4;7(40):66239-66254
pubmed: 27533466
Nat Commun. 2018 Sep 4;9(1):3588
pubmed: 30181541
Oncotarget. 2014 Dec 30;5(24):12528-42
pubmed: 25587024
Stem Cell Res Ther. 2021 Mar 16;12(1):183
pubmed: 33726849
J Cell Biol. 2000 Dec 11;151(6):1221-34
pubmed: 11121437
Cell. 2017 Dec 14;171(7):1611-1624.e24
pubmed: 29198524
Clin Cancer Res. 2021 Feb 1;27(3):852-864
pubmed: 33172898
Science. 2016 Apr 8;352(6282):189-96
pubmed: 27124452
Nat Genet. 2016 Mar;48(3):265-72
pubmed: 26829750
Physiol Rev. 2017 Oct 1;97(4):1235-1294
pubmed: 28794168

Auteurs

Anuraag S Parikh (AS)

Department of Otolaryngology, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032, USA.

Avishai Wizel (A)

The Lautenberg Center for Immunology and Cancer Research, IMRIC, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 9112102, Israel.

Daniel Davis (D)

The Lautenberg Center for Immunology and Cancer Research, IMRIC, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 9112102, Israel.

Armida Lefranc-Torres (A)

Department of Otolaryngology, Massachusetts Eye and Ear, Boston, MA 02114, USA.

Alejandro I Rodarte-Rascon (AI)

Department of Otolaryngology, Massachusetts Eye and Ear, Boston, MA 02114, USA.

Lauren E Miller (LE)

Department of Otolaryngology, Massachusetts Eye and Ear, Boston, MA 02114, USA; Department of Otolaryngology, Harvard Medical School, Boston, MA 02115, USA.

Kevin S Emerick (KS)

Department of Otolaryngology, Massachusetts Eye and Ear, Boston, MA 02114, USA; Department of Otolaryngology, Harvard Medical School, Boston, MA 02115, USA.

Mark A Varvares (MA)

Department of Otolaryngology, Massachusetts Eye and Ear, Boston, MA 02114, USA; Department of Otolaryngology, Harvard Medical School, Boston, MA 02115, USA.

Daniel G Deschler (DG)

Department of Otolaryngology, Massachusetts Eye and Ear, Boston, MA 02114, USA; Department of Otolaryngology, Harvard Medical School, Boston, MA 02115, USA.

William C Faquin (WC)

Department of Pathology, Massachusetts General Hospital, Boston, MA 02114, USA; Department of Pathology, Harvard Medical School, Boston, MA 02115, USA.

Jon C Aster (JC)

Department of Pathology, Harvard Medical School, Boston, MA 02115, USA; Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115, USA.

Derrick T Lin (DT)

Department of Otolaryngology, Massachusetts Eye and Ear, Boston, MA 02114, USA; Department of Otolaryngology, Harvard Medical School, Boston, MA 02115, USA.

Bradley E Bernstein (BE)

Department of Pathology, Harvard Medical School, Boston, MA 02115, USA; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA 02115, USA; Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA; Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.

Yotam Drier (Y)

The Lautenberg Center for Immunology and Cancer Research, IMRIC, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 9112102, Israel. Electronic address: yotam.drier@mail.huji.ac.il.

Sidharth V Puram (SV)

Department of Otolaryngology, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110, USA. Electronic address: sidpuram@wustl.edu.

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Classifications MeSH

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Recherchemedicale.com › Tumeurs › Tumeurs par type histologique › Tumeurs épithéliales épidermoïdes et glandulaires › Carcinomes › Adénocarcinome › Carcinome adénoïde kystique › Single-cell RNA sequencing identifies a...
Recherchemedicale.com › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Gènes tumoraux › Oncogènes › Single-cell RNA sequencing identifies a...
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Recherchemedicale.com › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ADN › Séquençage du génome entier › Séquençage de l'exome › Single-cell RNA sequencing identifies a...
Recherchemedicale.com › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ARN › Single-cell RNA sequencing identifies a...