Three Distinct Transcriptional Profiles of Monocytes Associate with Disease Activity in Scleroderma Patients.

Humans Monocytes / metabolism Scleroderma, Systemic / metabolism Scleroderma, Diffuse / genetics Macrophages / metabolism Biomarkers Skin / metabolism Scleroderma, Localized

Journal

Arthritis & rheumatology (Hoboken, N.J.)
ISSN: 2326-5205
Titre abrégé: Arthritis Rheumatol
Pays: United States
ID NLM: 101623795

Informations de publication

Date de publication:
04 2023
Historique:
revised: 23 09 2022
received: 28 02 2022
accepted: 06 10 2022
pmc-release: 01 04 2024
medline: 3 4 2023
pubmed: 26 10 2022
entrez: 25 10 2022
Statut: ppublish

Résumé

Patients with diffuse cutaneous systemic sclerosis (dcSSc) display a complex clinical phenotype. Transcriptional profiling of whole blood or tissue from patients are affected by changes in cellular composition that drive gene expression and an inability to detect minority cell populations. We undertook this study to focus on the 2 main subtypes of circulating monocytes, classical monocytes (CMs) and nonclassical monocytes (NCMs) as a biomarker of SSc disease severity. SSc patients were recruited from the Prospective Registry for Early Systemic Sclerosis. Clinical data were collected, as well as peripheral blood for isolation of CMs and NCMs. Age-, sex-, and race-matched healthy volunteers were recruited as controls. Bulk macrophages were isolated from the skin in a separate cohort. All samples were assayed by RNA sequencing (RNA-seq). We used an unbiased approach to cluster patients into 3 groups (groups A-C) based on the transcriptional signatures of CMs relative to controls. Each group maintained their characteristic transcriptional signature in NCMs. Genes up-regulated in group C demonstrated the highest expression compared to the other groups in SSc skin macrophages, relative to controls. Patients from groups B and C exhibited worse lung function than group A, although there was no difference in SSc skin disease at baseline, relative to controls. We validated our approach by applying our group classifications to published bulk monocyte RNA-seq data from SSc patients, and we found that patients without skin disease were most likely to be classified as group A. We are the first to show that transcriptional signatures of CMs and NCMs can be used to unbiasedly stratify SSc patients and correlate with disease activity outcome measures.

Identifiants

DOI: 10.1002/art.42380 PMID: 36281773 PMC: PMC10165944
pubmed: 36281773
doi: 10.1002/art.42380
pmc: PMC10165944
mid: NIHMS1843383
doi:

Substances chimiques

Biomarkers 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

595-608

Subventions

Organisme : NIAMS NIH HHS
ID : R21 AR074902
Pays : United States
Organisme : NIAMS NIH HHS
ID : K24 AR063120
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR073270
Pays : United States
Organisme : NCATS NIH HHS
ID : KL2 TR001424
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR075423
Pays : United States
Organisme : NIA NIH HHS
ID : P01 AG049665
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI135964
Pays : United States
Organisme : NIH HHS
ID : S10 OD026814
Pays : United States
Organisme : CSRD VA
ID : I01 CX001777
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA060553
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL134375
Pays : United States
Organisme : NIDDK NIH HHS
ID : P01 DK117824
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL152677
Pays : United States
Organisme : NIAMS NIH HHS
ID : K01 AR064313
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL147575
Pays : United States
Organisme : NIAMS NIH HHS
ID : T32 AR007611
Pays : United States
Organisme : NIAMS NIH HHS
ID : P30 AR072579
Pays : United States
Organisme : NIAMS NIH HHS
ID : R56 AR078211
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR073284
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI163742
Pays : United States

Informations de copyright

© 2022 The Authors. Arthritis & Rheumatology published by Wiley Periodicals LLC on behalf of American College of Rheumatology.

Références

Genome Med. 2017 Mar 23;9(1):27
pubmed: 28330499
Front Immunol. 2019 Dec 11;10:2880
pubmed: 31921144
Ann Rheum Dis. 2011 Jun;70(6):1003-9
pubmed: 21398330
Arthritis Care Res (Hoboken). 2022 Aug 23;:
pubmed: 35997480
Arthritis Res Ther. 2015 Jul 29;17:194
pubmed: 26220546
PLoS One. 2012;7(9):e45378
pubmed: 23028973
Science. 2016 Sep 9;353(6304):
pubmed: 27492475
Blood. 2005 Jul 1;106(1):207-15
pubmed: 15784728
J Med Case Rep. 2015 Jun 10;9:136
pubmed: 26055398
Ann Rheum Dis. 2020 Sep;79(9):1218-1226
pubmed: 32561607
J Invest Dermatol. 2012 May;132(5):1363-73
pubmed: 22318389
Eur J Immunol. 2020 Jan;50(1):119-129
pubmed: 31424086
Arthritis Res Ther. 2020 Mar 6;22(1):42
pubmed: 32143707
Nat Genet. 2012 Dec;44(12):1341-8
pubmed: 23143594
Immunity. 2013 Jan 24;38(1):79-91
pubmed: 23273845
Nature. 2013 Jul 18;499(7458):306-11
pubmed: 23868260
Cell. 2022 Apr 14;185(8):1373-1388.e20
pubmed: 35381199
Am J Pathol. 2018 Nov;188(11):2446-2456
pubmed: 30142332
J Invest Dermatol. 2018 Jun;138(6):1301-1310
pubmed: 29391252
Genome Biol. 2014;15(12):550
pubmed: 25516281
Ann Rheum Dis. 2020 Mar;79(3):379-386
pubmed: 31767698
J Dermatol Sci. 2013 Mar;69(3):250-8
pubmed: 23142052
J Exp Med. 2017 Aug 7;214(8):2387-2404
pubmed: 28694385
Scand J Immunol. 2013 Feb;77(2):162-8
pubmed: 23237063
Cell Rep. 2021 Aug 24;36(8):109607
pubmed: 34433035
J Invest Dermatol. 2013 Aug;133(8):1979-89
pubmed: 23677167
Arthritis Res Ther. 2010;12(4):R128
pubmed: 20602758
Dig Dis Sci. 2012 Jul;57(7):1822-31
pubmed: 22466098
J Invest Dermatol. 2018 Oct;138(10):2123-2132
pubmed: 29689251
Arthritis Res Ther. 2015 Aug 18;17:213
pubmed: 26283632
Arthritis Res Ther. 2015 Jun 13;17:159
pubmed: 26071192
Ann Rheum Dis. 2019 Apr;78(4):529-538
pubmed: 30793699
Arthritis Rheumatol. 2021 Jun;73(6):1005-1013
pubmed: 33350170
PLoS One. 2008 Jul 16;3(7):e2696
pubmed: 18648520
J Biomed Sci. 2015 Jan 16;22:6
pubmed: 25591788
Arthritis Rheumatol. 2015 Nov;67(11):3016-26
pubmed: 26238292
JCI Insight. 2016 Dec 22;1(21):e89073
pubmed: 28018971
Sci Rep. 2018 Jun 11;8(1):8856
pubmed: 29891910
Blood. 2011 Aug 4;118(5):e16-31
pubmed: 21653326
Proc Natl Acad Sci U S A. 2020 Oct 13;117(41):25712-25721
pubmed: 32989127
Ann Rheum Dis. 2020 Dec;79(12):1608-1615
pubmed: 32933919
PLoS One. 2015 Jan 21;10(1):e0114017
pubmed: 25607805
Arthritis Rheumatol. 2020 Nov;72(11):1892-1896
pubmed: 32583956
Immunity. 2018 Oct 16;49(4):595-613
pubmed: 30332628
Eur J Rheumatol. 2020 Oct;7(Suppl 3):S173-S180
pubmed: 33164732
Circ Res. 2020 May 8;126(10):e61-e79
pubmed: 32151196
Rheumatol Int. 2018 Mar;38(3):489-498
pubmed: 29356883
J Autoimmun. 2021 Jan;116:102547
pubmed: 33039247
Arthritis Rheumatol. 2022 Feb;74(2):329-341
pubmed: 34042322
J Rheumatol. 2017 Jun;44(6):791-794
pubmed: 28298560
Curr Rheumatol Rep. 2019 May 23;21(7):31
pubmed: 31123840
Proc Natl Acad Sci U S A. 2018 Apr 17;115(16):E3798-E3807
pubmed: 29610295
Am J Respir Crit Care Med. 2019 Jun 15;199(12):1517-1536
pubmed: 30554520
BMC Med. 2013 Jan 11;11:9
pubmed: 23311619

Auteurs

Hadijat-Kubura M Makinde (HM)

Division of Rheumatology, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.

Julia L M Dunn (JLM)

Division of Rheumatology, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, and Division of Allergy and Immunology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio.

Gaurav Gadhvi (G)

Division of Rheumatology, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.

Mary Carns (M)

Division of Rheumatology, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.

Kathleen Aren (K)

Division of Rheumatology, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.

Anh H Chung (AH)

Division of Rheumatology, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.

Lutfiyya N Muhammad (LN)

Department of Preventive Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.

Jing Song (J)

Department of Preventive Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.
ORCID: 0000-0002-8881-9664

Carla M Cuda (CM)

Division of Rheumatology, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.
ORCID: 0000-0002-2022-8171

Salina Dominguez (S)

Division of Rheumatology, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.

John E Pandolfino (JE)

Division of Gastroenterology and Hepatology, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.

Jane E Dematte D'Amico (JE)

Division of Division of Pulmonary and Critical Care, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.

G Scott Budinger (GS)

Division of Division of Pulmonary and Critical Care, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.

Shervin Assassi (S)

Prospective Registry of Early Systemic Sclerosis (PRESS) consortium, and Division of Rheumatology, University of Texas Health Science Center at Houston.
ORCID: 0000-0002-8059-9978

Tracy M Frech (TM)

PRESS consortium, and Division of Rheumatology and Immunology, Department of Medicine, Vanderbilt University, Nashville, Tennessee.
ORCID: 0000-0002-5472-3840

Dinesh Khanna (D)

PRESS consortium, and Division of Rheumatology, Department of Medicine, University of Michigan, Ann Arbor.
ORCID: 0000-0003-1412-4453

Alex Shaeffer (A)

Division of Rheumatology, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.

Harris Perlman (H)

Division of Rheumatology, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.
ORCID: 0000-0003-4174-608X

Monique Hinchcliff (M)

Division of Rheumatology, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, PRESS consortium, and Section of Rheumatology, Allergy & Immunology, School of Medicine, Yale University, New Haven, Connecticut.
ORCID: 0000-0002-8652-9890

Deborah R Winter (DR)

Division of Rheumatology, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Effectiveness of Virtual Yoga for Chronic Low Back Pain: A Randomized Clinical Trial.

Hallie Tankha, Devyn Gaskins, Amanda Shallcross et al.
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH

Recherchemedicale.com › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Three Distinct Transcriptional Profiles of...
Recherchemedicale.com › Systèmes sanguin et immunitaire › Système immunitaire › Phagocytes › Monocytes › Three Distinct Transcriptional Profiles of...
Recherchemedicale.com › Maladies de la peau et du tissu conjonctif › Maladies de la peau › Sclérodermie systémique › Three Distinct Transcriptional Profiles of...
Recherchemedicale.com › Maladies de la peau et du tissu conjonctif › Maladies de la peau › Sclérodermie systémique › Sclérodermie diffuse › Three Distinct Transcriptional Profiles of...
Recherchemedicale.com › Systèmes sanguin et immunitaire › Système immunitaire › Phagocytes › Macrophages › Three Distinct Transcriptional Profiles of...
Recherchemedicale.com › Facteurs biologiques › Marqueurs biologiques › Three Distinct Transcriptional Profiles of...
Recherchemedicale.com › Système tégumentaire › Peau › Three Distinct Transcriptional Profiles of...
Recherchemedicale.com › Maladies de la peau et du tissu conjonctif › Maladies de la peau › Sclérodermie localisée › Three Distinct Transcriptional Profiles of...