Purification, crystallization and crystallographic analysis of the PorX response regulator associated with the type IX secretion system.
PorX response regulator
bacterial pathogenesis
bacterial secretion systems
response regulators
two-component systems
type IX secretion system
Journal
Acta crystallographica. Section F, Structural biology communications
ISSN: 2053-230X
Titre abrégé: Acta Crystallogr F Struct Biol Commun
Pays: United States
ID NLM: 101620319
Informations de publication
Date de publication:
01 Oct 2022
01 Oct 2022
Historique:
received:
25
07
2022
accepted:
24
08
2022
entrez:
3
10
2022
pubmed:
4
10
2022
medline:
5
10
2022
Statut:
ppublish
Résumé
Pathogenic bacteria utilize specialized macromolecular secretion systems to transport virulence factors across membrane(s) and manipulate their infected host. To date, 11 secretion systems have been identified, including the type IX secretion system (T9SS) associated with human, avian and farmed-fish diseases. As a bacterial secretion system, the T9SS also facilitates gliding motility and the degradation of different macromolecules by the secretion of metabolic enzymes in nonpathogenic bacteria. PorX is a highly conserved protein that regulates the transcription of essential T9SS components and additionally mediates the function of T9SS via direct interaction with PorL, the rotary motor protein of the T9SS. PorX is also a member of a two-component system regulatory cascade, where it serves as the response regulator that relays a signal transduced from a conserved sensor histidine kinase, PorY, to a designated sigma factor. Here, the recombinant expression and purification of PorX homologous proteins from the pathogenic bacterium Porphyromonas gingivalis and the nonpathogenic bacterium Flavobacterium johnsoniae are reported. A bioinformatical characterization of the different domains comprising the PorX protein is also provided, and the crystallization and X-ray analysis of PorX from F. johnsoniae are reported.
Identifiants
pubmed: 36189719
pii: S2053230X22008500
doi: 10.1107/S2053230X22008500
pmc: PMC9527653
doi:
Substances chimiques
Bacterial Proteins
0
Bacterial Secretion Systems
0
Sigma Factor
0
Virulence Factors
0
Histidine Kinase
EC 2.7.13.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
354-362Subventions
Organisme : Alzheimer's Association
ID : AARGD-NTF-21-851231
Pays : United States
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