Phage-Plasmids Spread Antibiotic Resistance Genes through Infection and Lysogenic Conversion.
antibiotic resistance
bacteriophages
integrons
phage genomics
plasmids
prophage induction
Journal
mBio
ISSN: 2150-7511
Titre abrégé: mBio
Pays: United States
ID NLM: 101519231
Informations de publication
Date de publication:
26 10 2022
26 10 2022
Historique:
pubmed:
27
9
2022
medline:
29
10
2022
entrez:
26
9
2022
Statut:
ppublish
Résumé
Antibiotic resistance is rapidly spreading via the horizontal transfer of resistance genes in mobile genetic elements. While plasmids are key drivers of this process, few integrative phages encode antibiotic resistance genes. Here, we find that phage-plasmids, elements that are both phages and plasmids, often carry antibiotic resistance genes. We found 60 phage-plasmids with 184 antibiotic resistance genes, providing resistance for broad-spectrum-cephalosporins, carbapenems, aminoglycosides, fluoroquinolones, and colistin. These genes are in a few hot spots, seem to have been cotranslocated with transposable elements, and are often in class I integrons, which had not been previously found in phages. We tried to induce six phage-plasmids with resistance genes (including four with resistance integrons) and succeeded in five cases. Other phage-plasmids and integrative prophages were coinduced in these experiments. As a proof of concept, we focused on a P1-like element encoding an extended spectrum β-lactamase,
Identifiants
pubmed: 36154183
doi: 10.1128/mbio.01851-22
pmc: PMC9600943
doi:
Substances chimiques
Anti-Bacterial Agents
0
DNA Transposable Elements
0
Colistin
Z67X93HJG1
Aminoglycosides
0
beta-Lactamases
EC 3.5.2.6
Fluoroquinolones
0
Carbapenems
0
Cephalosporins
0
Plastics
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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