Magnetic Nanoparticle-Based Electrochemical Sensing Platform Using Ferrocene-Labelled Peptide Nucleic Acid for the Early Diagnosis of Colorectal Cancer.
biosensor
blood biomarkers
colorectal cancer (CRC)
magnetic nanoparticles
point-of-care diagnostic (POC)
Journal
Biosensors
ISSN: 2079-6374
Titre abrégé: Biosensors (Basel)
Pays: Switzerland
ID NLM: 101609191
Informations de publication
Date de publication:
07 Sep 2022
07 Sep 2022
Historique:
received:
08
08
2022
revised:
30
08
2022
accepted:
01
09
2022
entrez:
23
9
2022
pubmed:
24
9
2022
medline:
28
9
2022
Statut:
epublish
Résumé
Diagnostic biomarkers based on epigenetic changes such as DNA methylation are promising tools for early cancer diagnosis. However, there are significant difficulties in directly and specifically detecting methylated DNA regions. Here, we report an electrochemical sensing system based on magnetic nanoparticles that enable a quantitative and selective analysis of the methylated septin9 (mSEPT9) gene, which is considered a diagnostic marker in early stage colorectal cancer (CRC). Methylation levels of SEPT9 in CRC samples were successfully followed by the selective recognition ability of a related peptide nucleic acid (PNA) after hybridization with DNA fragments in human patients' serums and plasma (
Identifiants
pubmed: 36140121
pii: bios12090736
doi: 10.3390/bios12090736
pmc: PMC9496070
pii:
doi:
Substances chimiques
Amino Acids, Branched-Chain
0
Biomarkers, Tumor
0
Ferrous Compounds
0
Magnetite Nanoparticles
0
Metallocenes
0
Peptide Nucleic Acids
0
Transcription Factors
0
DNA
9007-49-2
BCAT1 protein, human
EC 2.6.1.
Transaminases
EC 2.6.1.-
Septins
EC 3.6.1.-
ferrocene
U96PKG90JQ
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Scientific and Technological Research Council of Turkey
ID : 219S405
Références
Analyst. 2007 Aug;132(8):784-91
pubmed: 17646878
J Am Chem Soc. 2010 Jun 30;132(25):8676-81
pubmed: 20527745
Anal Chim Acta. 2018 Dec 14;1038:157-165
pubmed: 30278898
Int J Mol Sci. 2020 Jul 27;21(15):
pubmed: 32726923
ACS Omega. 2020 Jun 25;5(26):16228-16237
pubmed: 32656445
Nanoscale Res Lett. 2012 Feb 21;7(1):144
pubmed: 22348683
Chem Soc Rev. 2019 Feb 18;48(4):1004-1076
pubmed: 30534770
Diagnostics (Basel). 2022 Aug 15;12(8):
pubmed: 36010323
Pathol Oncol Res. 2011 Sep;17(3):503-9
pubmed: 21267688
Nat Genet. 2007 Apr;39(4):457-66
pubmed: 17334365
Bioconjug Chem. 2000 Nov-Dec;11(6):741-3
pubmed: 11087319
PLoS One. 2012;7(9):e46000
pubmed: 23049919
BMC Cancer. 2013 Aug 30;13:398
pubmed: 23988185
World J Clin Cases. 2018 Dec 6;6(15):869-881
pubmed: 30568941
Cancer Med. 2016 Oct;5(10):2763-2772
pubmed: 27726312
BMC Cancer. 2019 May 14;19(1):450
pubmed: 31088406
CA Cancer J Clin. 2021 May;71(3):209-249
pubmed: 33538338
N Engl J Med. 2009 Sep 17;361(12):1179-87
pubmed: 19759380
Biosens Bioelectron. 1998 Oct 1;13(7-8):757-62
pubmed: 9828369
Anal Chem. 2018 May 1;90(9):5850-5856
pubmed: 29617110
Cancer Epidemiol Biomarkers Prev. 2020 Dec;29(12):2702-2709
pubmed: 32958500
Adv Clin Chem. 2015;72:171-204
pubmed: 26471083
Reprod Sci. 2021 Feb;28(2):532-540
pubmed: 33095426
Chem Asian J. 2022 Jul 15;17(14):e202200287
pubmed: 35471591
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2022 Jan;14(1):e1765
pubmed: 34734485
Genes (Basel). 2016 Dec 15;7(12):
pubmed: 27983717
Sensors (Basel). 2015 Sep 03;15(9):22291-303
pubmed: 26404293
Mikrochim Acta. 2021 Aug 27;188(9):307
pubmed: 34453211
Anal Chem. 2020 Jan 7;92(1):1033-1040
pubmed: 31800231
Sci Rep. 2017 Jan 19;7:40737
pubmed: 28102343
Chembiochem. 2010 Aug 16;11(12):1754-61
pubmed: 20602405
Talanta. 2019 Aug 15;201:465-473
pubmed: 31122452
Eur J Med Res. 2017 Nov 29;22(1):51
pubmed: 29187262
Analyst. 2014 Feb 21;139(4):724-8
pubmed: 24384604
Talanta. 2022 Jun 1;243:123356
pubmed: 35248943