Knock-in mouse models for studying somatostatin and cholecystokinin expressing cells.

Animals Cholecystokinin / genetics Codon, Initiator Codon, Terminator Mice Mice, Inbred C57BL Mice, Transgenic Somatostatin / genetics
CRISPR/Cas9 Cholecystokinin Cre Easi-CRISPR Somatostatin ZsGreen1

Journal

Journal of neuroscience methods
ISSN: 1872-678X
Titre abrégé: J Neurosci Methods
Pays: Netherlands
ID NLM: 7905558

Informations de publication

Date de publication:
01 11 2022
Historique:
received: 18 07 2022
revised: 29 08 2022
accepted: 01 09 2022
pubmed: 8 9 2022
medline: 5 10 2022
entrez: 7 9 2022
Statut: ppublish

Résumé

Somatostatin (SST) and cholecystokinin (CCK) are peptide hormones that regulate the endocrine system, cell proliferation and neurotransmission. We utilized the novel Easi-CRISPR system to generate two knock-in mouse strains with Cre recombinase in SST- and CCK-expressing cells and validated their utility in the developing and adult brain tissues. The full nomenclature for the newly generated strains are C57BL/6-Sst Knock-in mouse models to study cell types that produce these critically important molecules are limited to date. The knock-in mice we generated can be used as reporters to study development, physiology, or pathophysiology of SST and CCK expressing cells - without interference with native expression of SST and CCK. In addition, they can be used as Cre driver models to conditionally delete floxed genes in SST and CCK expressing cells across various tissues. These two mouse models serve as valuable tools for in vitro and in vivo research studies related to SST and CCK biology across the lifespan and across different tissue types.

Sections du résumé

BACKGROUND
Somatostatin (SST) and cholecystokinin (CCK) are peptide hormones that regulate the endocrine system, cell proliferation and neurotransmission.
NEW METHOD
We utilized the novel Easi-CRISPR system to generate two knock-in mouse strains with Cre recombinase in SST- and CCK-expressing cells and validated their utility in the developing and adult brain tissues.
RESULTS
The full nomenclature for the newly generated strains are C57BL/6-Sst
COMPARISON WITH EXISTING METHODS
Knock-in mouse models to study cell types that produce these critically important molecules are limited to date. The knock-in mice we generated can be used as reporters to study development, physiology, or pathophysiology of SST and CCK expressing cells - without interference with native expression of SST and CCK. In addition, they can be used as Cre driver models to conditionally delete floxed genes in SST and CCK expressing cells across various tissues.
CONCLUSIONS
These two mouse models serve as valuable tools for in vitro and in vivo research studies related to SST and CCK biology across the lifespan and across different tissue types.

Identifiants

DOI: 10.1016/j.jneumeth.2022.109704 PMID: 36070817
pubmed: 36070817
pii: S0165-0270(22)00230-8
doi: 10.1016/j.jneumeth.2022.109704
pii:
doi:

Substances chimiques

Codon, Initiator 0
Codon, Terminator 0
Somatostatin 51110-01-1
Cholecystokinin 9011-97-6

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

109704

Subventions

Organisme : NIMH NIH HHS
ID : R01 MH067234
Pays : United States

Informations de copyright

Copyright © 2022 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Conflict of Interest The authors declare no conflict of interest.

Auteurs

Marta Balog (M)

Munroe-Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center Omaha, NE, USA; Department of Medical Biology and Genetics, Faculty of Medicine, University of Osijek, Osijek, Croatia.

Allison Anderson (A)

Munroe-Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center Omaha, NE, USA.

Channabasavaiah B Gurumurthy (CB)

Mouse Genome Engineering Core Facility, University of Nebraska Medical Center, Omaha, NE, USA; Department of Pharmacology and Experimental Neuroscience, College of Medicine, University of Nebraska Medical Center, Omaha, NE, USA.

Rolen M Quadros (RM)

Mouse Genome Engineering Core Facility, University of Nebraska Medical Center, Omaha, NE, USA.

Zeljka Korade (Z)

Department of Pediatrics, University of Nebraska Medical Center Omaha, NE, USA; Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center Omaha, NE, USA; Child Health Research Institute, University of Nebraska Medical Center Omaha, NE, USA. Electronic address: zeljka.korade@unmc.edu.

Karoly Mirnics (K)

Munroe-Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center Omaha, NE, USA; Department of Pharmacology and Experimental Neuroscience, College of Medicine, University of Nebraska Medical Center, Omaha, NE, USA; Department of Pediatrics, University of Nebraska Medical Center Omaha, NE, USA; Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center Omaha, NE, USA; Child Health Research Institute, University of Nebraska Medical Center Omaha, NE, USA. Electronic address: karoly.mirnics@unmc.edu.

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Classifications MeSH

Recherchemedicale.com › Eucaryotes › Animaux › Knock-in mouse models for studying...
Recherchemedicale.com › Acides aminés, peptides et protéines › Peptides › Cholécystokinine › Knock-in mouse models for studying...
Recherchemedicale.com › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Composants de gène › Codon › Codon d'initiation › Knock-in mouse models for studying...
Recherchemedicale.com › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Composants de gène › Codon › Codon stop › Knock-in mouse models for studying...
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Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Protéines de tissu nerveux › Neuropeptides › Hormones hypothalamiques › Hormones hypothalamiques hypophysotropes inhibitrices › Somatostatine › Knock-in mouse models for studying...